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A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish
Cai-Hua Dong; Shu-Ting Yang; Zhong-An Yang; Lei Zhang and Jian-Fang Gui; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004
Source PublicationDEVELOPMENTAL BIOLOGY
Volume265Issue:2Pages:341-354
AbstractOocyte maturation and egg fertilization in both vertebrates and invertebrates are marked by orchestrated cytoplasmic translocation of secretory vesicles known as cortical granules. It is thought that such redistribution of cellular content is critical for asymmetrical cell division during early development, but the mechanism and regulation of the process is poorly understood. Here we report the identification, purification and cDNA cloning of a C-type lectin from oocytes of a freshwater fish species gibel carp (Carassius auratus gibelio). The purified protein has been demonstrated to have lectin activity and to be a Ca2+-dependent C-type lectin by hemagglutination activity assay. Immunocytochemistry revealed that the lectin is associated with cortical granules, gradually translocated to the cell surface during oocyte maturation, and discharged to the egg envelope upon fertilization. Interestingly, the lectin becomes phosphorylated on threonine residues upon induction of exocytosis by fertilization and returns to its original state after morula stage of embryonic development, suggesting that this posttranslational modification may represent a critical molecular switch for early embryonic development. (C) 2003 Elsevier Inc. All rights reserved.
KeywordLectin Cortical Granule Translocation Phosphorylation Gibel Carp
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaDevelopmental Biology
Indexed BySCI
WOS IDWOS:000188931000006
Citation statistics
Cited Times:79[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9554
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Cai-Hua Dong,Shu-Ting Yang,Zhong-An Yang,et al. A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish[J]. DEVELOPMENTAL BIOLOGY,2004,265(2):341-354.
APA Cai-Hua Dong,Shu-Ting Yang,Zhong-An Yang,Lei Zhang and Jian-Fang Gui,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish.DEVELOPMENTAL BIOLOGY,265(2),341-354.
MLA Cai-Hua Dong,et al."A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish".DEVELOPMENTAL BIOLOGY 265.2(2004):341-354.
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