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学科主题: Developmental Biology
题名: A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish
作者: Cai-Hua Dong ; Shu-Ting Yang ; Zhong-An Yang ; Lei Zhang and Jian-Fang Gui
通讯作者: Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: lectin ; cortical granule ; translocation ; phosphorylation ; gibel carp
刊名: DEVELOPMENTAL BIOLOGY
发表日期: 2004
卷: 265, 期:2, 页:341-354
收录类别: SCI
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol, Wuhan 430072, Peoples R China
摘要: Oocyte maturation and egg fertilization in both vertebrates and invertebrates are marked by orchestrated cytoplasmic translocation of secretory vesicles known as cortical granules. It is thought that such redistribution of cellular content is critical for asymmetrical cell division during early development, but the mechanism and regulation of the process is poorly understood. Here we report the identification, purification and cDNA cloning of a C-type lectin from oocytes of a freshwater fish species gibel carp (Carassius auratus gibelio). The purified protein has been demonstrated to have lectin activity and to be a Ca2+-dependent C-type lectin by hemagglutination activity assay. Immunocytochemistry revealed that the lectin is associated with cortical granules, gradually translocated to the cell surface during oocyte maturation, and discharged to the egg envelope upon fertilization. Interestingly, the lectin becomes phosphorylated on threonine residues upon induction of exocytosis by fertilization and returns to its original state after morula stage of embryonic development, suggesting that this posttranslational modification may represent a critical molecular switch for early embryonic development. (C) 2003 Elsevier Inc. All rights reserved.
WOS记录号: WOS:000188931000006
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9554
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Cai-Hua Dong; Shu-Ting Yang; Zhong-An Yang; Lei Zhang and Jian-Fang Gui.A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish,DEVELOPMENTAL BIOLOGY,2004,265(2):341-354
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