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学科主题: Fisheries; Veterinary Sciences
题名: Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines
作者: Qi-Ya Zhang ; Hong-Mei Ruan ; Zhen-Qiu Li ; Xiu-Ping Yuan ; Jian-Fang Gui
通讯作者: Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: lymphocystis virus ; LV ; iridovirus ; viral disease ; freshwater fish ; cell line ; apoptosis
刊名: DISEASES OF AQUATIC ORGANISMS
发表日期: 2003
卷: 57, 期:1-2, 页:27-34
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: The causative agent of lymphocystis disease that frequently occurs in cultured flounder Paralichthys olivaceus in China is lymphocystis virus (LV). In this study, 13 fish cell lines were tested for their susceptibility to LV. Of these, 2 cell lines derived from the freshwater grass carp Ctenopharyngodon idellus proved susceptible to the LV, and 1 cell line, GCO (grass carp ovary), was therefore used to replicate and propagate the virus. An obvious cytopathic effect (CPE) was first observed in cell monolayers at 1 d post-inoculation, and at 3 d this had extended to about 75% of the cell monolayer. However, no further CPE extension was observed after 4 d. Cytopathic characteristics induced by the LV were detected by Giemsa staining and fluorescence microscopic observation with Hoechst 33258 staining. The propagated virus particles were also observed by electron microscopy. Ultrastructure analysis revealed several distinct cellular changes, such as chromatin compaction and margination, vesicle formation, cell-surface convolution, nuclear fragmentation and the occurrence of characteristic 'blebs' and cell fusion. This study provides a detailed report of LV infection and propagation in a freshwater fish cell line, and presents direct electron microscopy evidence for propagation of the virus in infected cells. A possible process by which the CPEs are controlled is suggested.
语种: 英语
WOS记录号: WOS:000189277700004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9546
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Qi-Ya Zhang; Hong-Mei Ruan; Zhen-Qiu Li; Xiu-Ping Yuan; Jian-Fang Gui.Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines,DISEASES OF AQUATIC ORGANISMS,2003,57(1-2):27-34
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