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Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines
Qi-Ya Zhang; Hong-Mei Ruan; Zhen-Qiu Li; Xiu-Ping Yuan; Jian-Fang Gui; Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2003
Source PublicationDISEASES OF AQUATIC ORGANISMS
Volume57Issue:1-2Pages:27-34
AbstractThe causative agent of lymphocystis disease that frequently occurs in cultured flounder Paralichthys olivaceus in China is lymphocystis virus (LV). In this study, 13 fish cell lines were tested for their susceptibility to LV. Of these, 2 cell lines derived from the freshwater grass carp Ctenopharyngodon idellus proved susceptible to the LV, and 1 cell line, GCO (grass carp ovary), was therefore used to replicate and propagate the virus. An obvious cytopathic effect (CPE) was first observed in cell monolayers at 1 d post-inoculation, and at 3 d this had extended to about 75% of the cell monolayer. However, no further CPE extension was observed after 4 d. Cytopathic characteristics induced by the LV were detected by Giemsa staining and fluorescence microscopic observation with Hoechst 33258 staining. The propagated virus particles were also observed by electron microscopy. Ultrastructure analysis revealed several distinct cellular changes, such as chromatin compaction and margination, vesicle formation, cell-surface convolution, nuclear fragmentation and the occurrence of characteristic 'blebs' and cell fusion. This study provides a detailed report of LV infection and propagation in a freshwater fish cell line, and presents direct electron microscopy evidence for propagation of the virus in infected cells. A possible process by which the CPEs are controlled is suggested.
KeywordLymphocystis Virus Lv Iridovirus Viral Disease Freshwater Fish Cell Line Apoptosis
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Veterinary Sciences
Indexed BySCI
Language英语
WOS IDWOS:000189277700004
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9546
Collection期刊论文
Corresponding AuthorGui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Qi-Ya Zhang,Hong-Mei Ruan,Zhen-Qiu Li,et al. Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines[J]. DISEASES OF AQUATIC ORGANISMS,2003,57(1-2):27-34.
APA Qi-Ya Zhang,Hong-Mei Ruan,Zhen-Qiu Li,Xiu-Ping Yuan,Jian-Fang Gui,&Gui, HF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2003).Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines.DISEASES OF AQUATIC ORGANISMS,57(1-2),27-34.
MLA Qi-Ya Zhang,et al."Infection and propagation of lymphocystis virus isolated from the cultured flounder Paralichthys olivaceus in grass carp cell lines".DISEASES OF AQUATIC ORGANISMS 57.1-2(2003):27-34.
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