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学科主题: Virology
题名: Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
作者: Wang, HL; Wu, D; Deng, F; Peng, HY; Chen, XW; Lauzon, H; Arif, BM; Jehle, JA; Hu, ZH
通讯作者: Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
关键词: HaSNPV ; chitinase ; characterization ; phylogenetic analysis ; secondary structure analysis
刊名: VIRUS RESEARCH
发表日期: 2004-03-15
DOI: 10.1016/j.virusres.2003.11.015
卷: 100, 期:2, 页:179-189
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China; Great Lakes Forestry Res Ctr, Mol Virol Lab, Sault Ste Marie, ON P6A 5M7, Canada; State Educ & Res Ctr Agr Viticulture & Hort, Sect Biotechnol Crop Protect, D-67435 Neustadt, Germany
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Virology
研究领域[WOS]: Virology
摘要: A putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要: A putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: NUCLEAR POLYHEDROSIS-VIRUS ; PROTEIN SECONDARY STRUCTURE ; CARBOXY-TERMINAL KDEL ; ENDOPLASMIC-RETICULUM ; SEQUENCE-ANALYSIS ; BACULOVIRUS ; GENOME ; IDENTIFICATION ; RESOLUTION ; ENCODES
语种: 英语
WOS记录号: WOS:000220326400004
ISSN号: 0168-1702
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9536
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China
4.Great Lakes Forestry Res Ctr, Mol Virol Lab, Sault Ste Marie, ON P6A 5M7, Canada
5.State Educ & Res Ctr Agr Viticulture & Hort, Sect Biotechnol Crop Protect, D-67435 Neustadt, Germany

Recommended Citation:
.Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus,VIRUS RESEARCH,2004,100(2):179-189
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