IHB OpenIR  > 期刊论文
Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
Wang, HL; Wu, D; Deng, F; Peng, HY; Chen, XW; Lauzon, H; Arif, BM; Jehle, JA; Hu, ZH; Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
2004-03-15
Source PublicationVIRUS RESEARCH
ISSN0168-1702
Volume100Issue:2Pages:179-189
AbstractA putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved.; A putative chitinase gene was identified within the fragment EcoRI-K of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV, also called HaSNPIV) genome. The open reading frame (ORF) contains 1713 nucleotides (nt) and encodes a protein of 570 amino acids (aa) with a predicted molecular weight of 63.6 kDa. Transcription started at about 18 h post infection (p.i.) and the protein was first detected at 20 h p.i. The times of transcription and expression are characteristic of a late baculovirus gene. 5' and 3' RACE indicated that transcription was initiated from the adenine residue located at -246 nt upstream from the ATG start site and the poly (A) tail was added at 267 nt downstream from the stop codon. This is the first report on the molecular characterization of a chitinase from a single nucleocapsid NPV. The phylogeny of baculoviral chitinase genes were extensively examined in comparison with chitinases derived from bacteria, fungi, nematode, actinomycetes, viruses, insects and mammals. Neighbor-joining and most parsimony analyses showed that the baculoviral chitinases were clustered exclusively within gamma-proteobacteria. Our results strongly suggest that baculoviruses acquired their chitinase genes from bacteria. (C) 2004 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordHasnpv Chitinase Characterization Phylogenetic Analysis Secondary Structure Analysis
DepartmentChinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China; Great Lakes Forestry Res Ctr, Mol Virol Lab, Sault Ste Marie, ON P6A 5M7, Canada; State Educ & Res Ctr Agr Viticulture & Hort, Sect Biotechnol Crop Protect, D-67435 Neustadt, Germany
Subject AreaVirology
DOI10.1016/j.virusres.2003.11.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000220326400004
WOS KeywordNUCLEAR POLYHEDROSIS-VIRUS ; PROTEIN SECONDARY STRUCTURE ; CARBOXY-TERMINAL KDEL ; ENDOPLASMIC-RETICULUM ; SEQUENCE-ANALYSIS ; BACULOVIRUS ; GENOME ; IDENTIFICATION ; RESOLUTION ; ENCODES
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9536
Collection期刊论文
Corresponding AuthorHu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
Affiliation1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Mol Virol, Wuhan 430071, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China
4.Great Lakes Forestry Res Ctr, Mol Virol Lab, Sault Ste Marie, ON P6A 5M7, Canada
5.State Educ & Res Ctr Agr Viticulture & Hort, Sect Biotechnol Crop Protect, D-67435 Neustadt, Germany
Recommended Citation
GB/T 7714
Wang, HL,Wu, D,Deng, F,et al. Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus[J]. VIRUS RESEARCH,2004,100(2):179-189.
APA Wang, HL.,Wu, D.,Deng, F.,Peng, HY.,Chen, XW.,...&Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, Wuhan 430071, Peoples R China.(2004).Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.VIRUS RESEARCH,100(2),179-189.
MLA Wang, HL,et al."Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus".VIRUS RESEARCH 100.2(2004):179-189.
Files in This Item:
File Name/Size DocType Version Access License
Characterization and(851KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, HL]'s Articles
[Wu, D]'s Articles
[Deng, F]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, HL]'s Articles
[Wu, D]'s Articles
[Deng, F]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, HL]'s Articles
[Wu, D]'s Articles
[Deng, F]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Characterization and phylogenetic analysis of the chitinase gene from the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.