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学科主题: Virology
题名: Induction of apoptosis in a carp leucocyte cell line infected with turbot (Scophthalmus maximus L.) rhabdovirus
作者: Changsheng Du ; Qiya Zhang ; Chunliang Li ; Dali Miao ; Jianfang Gui
通讯作者: Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Scophthalmus maximus rhabdovirus (SMRV) ; apoptosis ; carp leucocyte (CLC) cells ; flow cytometry ; apoptotic peak
刊名: VIRUS RESEARCH
发表日期: 2004
卷: 101, 期:2, 页:119-126
收录类别: SCI
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
摘要: A rhabdovirus was observed from the diseased turbot (Scophthalmus maximus L.) with lethal syndrome. In this study, a carp leucocyte (CLC) cell line was used to investigate the infection process and cell death mechanism occurring during the virus infection. Strong cytopathogenic effect (CPE) and the morphological changes, such as extreme chromatin condensation, nucleus fragmentation, and apoptotic body formation, were observed under fluorescence microscopy after DAPI staining in the infected CLC cells. Transmission electron microscopy analysis showed cell shrinkage, plasma membrane blebbing, cytoplasm vacuolization, chromatin condensation, nuclear breakdown and formation of discrete apoptotic bodies. The bullet-shaped nucleocapsids were measured and ranged in size from 110 to 150 nm in length and 40 to 60 nm in diameter. And therefore the virus is called Scophthalmus maximus rhabdovirus (SMRV). Agarose gel electrophoresis analysis of the DNA extracted from infected cells showed typical DNA ladder in the course of SMRV infection. Flow cytometry analysis of SMRV infected CLC cells detected apoptotic peak in the virus infected CLC cells. Virus titre analysis and electron microscopic observation revealed that the virus replication fastigium was earlier than that of the apoptosis occurrence. No apoptosis was observed in the CLC infected with UV-inactivated SMRV. All these supported that SMRV infected CLC cells undergo apoptosis and the virus replication is necessary for apoptosis induction of CLC cells. (C) 2004 Published by Elsevier B.V.
语种: 英语
WOS记录号: WOS:000220841200003
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9514
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Changsheng Du; Qiya Zhang; Chunliang Li; Dali Miao; Jianfang Gui.Induction of apoptosis in a carp leucocyte cell line infected with turbot (Scophthalmus maximus L.) rhabdovirus,VIRUS RESEARCH,2004,101(2):119-126
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