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EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas
Wan, XQ; Ma, TW; Wu, WZ; Wang, ZJ; Wan, XQ, Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China
2004-05-01
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
Volume58Issue:1Pages:84-89
AbstractAryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin-O-deethylase (FROD) assay of a primary hepatocyte culture from grass carp (Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose-response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC50 = 0.83 mug/mL) is more sensitive to TCDD than the assay based on primary hepatocyte Culture (EC50 = 9.7 pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. (C) 2004 Elsevier Inc. All rights reserved.; Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin-O-deethylase (FROD) assay of a primary hepatocyte culture from grass carp (Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose-response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC50 = 0.83 mug/mL) is more sensitive to TCDD than the assay based on primary hepatocyte Culture (EC50 = 9.7 pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. (C) 2004 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordPrimary Hepatocyte Culture Erod Grass Carp (Ctenopharyngodon Idellus) Polychlorinated Aromatic Hydrocarbons
DepartmentChinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaEnvironmental Sciences ; Toxicology
DOI10.1016/j.ecoenv.2004.01.005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000221164800012
WOS KeywordRAINBOW-TROUT HEPATOCYTES ; EPITHELIAL-CELLS ; AH RECEPTOR ; LIVER-CELLS ; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN ; BINDING ; FISH ; EXPRESSION ; TOXICITY ; MODEL
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Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9498
Collection期刊论文
Corresponding AuthorWan, XQ, Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wan, XQ,Ma, TW,Wu, WZ,et al. EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2004,58(1):84-89.
APA Wan, XQ,Ma, TW,Wu, WZ,Wang, ZJ,&Wan, XQ, Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China.(2004).EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,58(1),84-89.
MLA Wan, XQ,et al."EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 58.1(2004):84-89.
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