| EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas | |
| Wan, XQ; Ma, TW; Wu, WZ; Wang, ZJ; Wan, XQ, Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China | |
| 2004-05-01 | |
| Source Publication | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| ISSN | 0147-6513 |
| Volume | 58Issue:1Pages:84-89 |
| Abstract | Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin-O-deethylase (FROD) assay of a primary hepatocyte culture from grass carp (Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose-response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC50 = 0.83 mug/mL) is more sensitive to TCDD than the assay based on primary hepatocyte Culture (EC50 = 9.7 pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. (C) 2004 Elsevier Inc. All rights reserved.; Aryl hydrocarbon (Ah) receptor (Ah-agonist) effects of environmental samples containing polychlorinated aromatic hydrocarbons were evaluated using a 7-ethoxyresorufin-O-deethylase (FROD) assay of a primary hepatocyte culture from grass carp (Ctenopharyngodon idellus). The results were compared with those obtained from the assay using the rat hepatoma cell line H4IIE and chemical analysis using high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS). A dose-response relationship was observed between the EROD activities, either from primary hepatocyte culture assay or from H4IIE assay, and concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results showed that the assay based on the H4IIE cell line (EC50 = 0.83 mug/mL) is more sensitive to TCDD than the assay based on primary hepatocyte Culture (EC50 = 9.7 pg/mL). In tests of environmental samples, the results from the assay using primary hepatocyte culture were comparable to those from the assay using the H4IIE cell line and chemical analysis of concentrations of mixtures of polychlorinated dibenzo-p-dioxin and dibenzofuran (PCDD/PCDF). The lack of a change in the activities of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) in cell culture upon exposure to TCDD indirectly indicates that the compound is persistent to biodegradation in the cell culture system. (C) 2004 Elsevier Inc. All rights reserved. |
| Subtype | Article |
| Keyword | Primary Hepatocyte Culture Erod Grass Carp (Ctenopharyngodon Idellus) Polychlorinated Aromatic Hydrocarbons |
| Department | Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Subject Area | Environmental Sciences ; Toxicology |
| DOI | 10.1016/j.ecoenv.2004.01.005 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology ; Toxicology |
| WOS Subject | Environmental Sciences ; Toxicology |
| WOS ID | WOS:000221164800012 |
| WOS Keyword | RAINBOW-TROUT HEPATOCYTES ; EPITHELIAL-CELLS ; AH RECEPTOR ; LIVER-CELLS ; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN ; BINDING ; FISH ; EXPRESSION ; TOXICITY ; MODEL |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9498 |
| Collection | 期刊论文 |
| Corresponding Author | Wan, XQ, Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Wan, XQ,Ma, TW,Wu, WZ,et al. EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2004,58(1):84-89. |
| APA | Wan, XQ,Ma, TW,Wu, WZ,Wang, ZJ,&Wan, XQ, Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, POB 2871, Beijing 100085, Peoples R China.(2004).EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,58(1),84-89. |
| MLA | Wan, XQ,et al."EROD activities in a primary cell culture of grass carp (Ctenopharyngodon idellus) hepatocytes exposed to polychlorinated aromatic hydrocarbonas".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 58.1(2004):84-89. |
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