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Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus
Yu, XY; Mao, Y; Wang, MF; Zhou, L; Gui, JF; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004-04-01
Source PublicationJOURNAL OF SHELLFISH RESEARCH
ISSN0730-8000
Volume23Issue:1Pages:165-171
AbstractPen shell (Atrina pectinata Linnaeus) can be distinguished into four forms based on the morphololgic characteristics. Genetic similarity, and heterogeneity were analyzed among the four forms by random amplified polymorphic DNA (RAPD) technique using 24 10-nucleotide-long primers. Of these primers, 22 pruners produced well-identifiable RAPD band patterns. Significant differences in RAPD band patterns were revealed among the four forms. A total of 198 polymorphic fragments were scored from 22 pruners. and they are specific for one form, shared by two or three forms. Several pruners, such as S451, S453 S463 S464, S470. S473 and S474, produced abundant band patterns and provided sufficient information for reliable discrimination of the four forms. The average genetic distances and phylogenetic relationships were calculated and analyzed according to the distinguishable fragments. The data indicate that pen shells of form G and form Y are similar not only among individuals within the same form, but also between individuals from the two forms, and that shells of form T and form S are highly divergent. The constructed phylogenetic free matches the average genetic distances. Three clusters were clearly distinguishable, in which two were corresponding to form S and form T respectively and one included forms G and Y. This Study will be benefit to further studies oil the taxonomy and selective breeding of Pinnid species. It is suggested that the four forms of pen shell should be categorized to at least two species taxonomically.; Pen shell (Atrina pectinata Linnaeus) can be distinguished into four forms based on the morphololgic characteristics. Genetic similarity, and heterogeneity were analyzed among the four forms by random amplified polymorphic DNA (RAPD) technique using 24 10-nucleotide-long primers. Of these primers, 22 pruners produced well-identifiable RAPD band patterns. Significant differences in RAPD band patterns were revealed among the four forms. A total of 198 polymorphic fragments were scored from 22 pruners. and they are specific for one form, shared by two or three forms. Several pruners, such as S451, S453 S463 S464, S470. S473 and S474, produced abundant band patterns and provided sufficient information for reliable discrimination of the four forms. The average genetic distances and phylogenetic relationships were calculated and analyzed according to the distinguishable fragments. The data indicate that pen shells of form G and form Y are similar not only among individuals within the same form, but also between individuals from the two forms, and that shells of form T and form S are highly divergent. The constructed phylogenetic free matches the average genetic distances. Three clusters were clearly distinguishable, in which two were corresponding to form S and form T respectively and one included forms G and Y. This Study will be benefit to further studies oil the taxonomy and selective breeding of Pinnid species. It is suggested that the four forms of pen shell should be categorized to at least two species taxonomically.
SubtypeArticle
KeywordRapd Inarker Genetic Polymorphism Taxonorny Atrina Pectinata
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Zhanjiang Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000221403900023
WOS KeywordAURATUS-GIBELIO BLOCH ; SILVER CRUCIAN CARP ; POPULATION-GENETICS ; ARBITRARY PRIMERS ; FAMILY OSTREIDAE ; CRASSOSTREA ; SACCOSTREA ; CLONES ; PCR
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9484
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Zhanjiang Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China
Recommended Citation
GB/T 7714
Yu, XY,Mao, Y,Wang, MF,et al. Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus[J]. JOURNAL OF SHELLFISH RESEARCH,2004,23(1):165-171.
APA Yu, XY,Mao, Y,Wang, MF,Zhou, L,Gui, JF,&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus.JOURNAL OF SHELLFISH RESEARCH,23(1),165-171.
MLA Yu, XY,et al."Genetic heterogeneity analysis and RAPD marker detection among four forms of Atrina pectinata Linnaeus".JOURNAL OF SHELLFISH RESEARCH 23.1(2004):165-171.
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