Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi City 214081, Jiangsu, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Dept Chinese River Dolphin, Wuhan 430072, Hubei, Peoples R China; Shinshu Univ, Fac Sci, Dept Environm Sci, Matsumoto, Nagano 3908621, Japan; Ehime Univ, Ctr Marine Environm Studies, Matsuyama, Ehime 7908577, Japan; Univ Tokyo, Ocean Res Inst, Ctr Int Cooperat, Nakano Ku, Tokyo 1648639, Japan
Total and subcellular hepatic Zn, Cu, Se, Mn, V, Hg, Cd, and Ag were determined in a mother-fetus pair of Dall's porpoises (Phocoenoides dalli). Except for higher fetal Cu concentration, all maternal elements were higher. Elements existed mostly in the cytosol of both animals except in the case of maternal Ag in the microsome and fetal Cu and Ag in the nuclei and mitochondria. In the maternal cytosol, Zn, Mn, Hg, and Ag were present in the high-molecular-weight substances (HMW); Se and V were present in the low-molecular-weight substances (LMW); Cu and Cd were mostly sequestered by metallothionein (MT). In the fetal cytosol, Zn, Se, Mn, Hg, Cd, and Ag were present in the HMW and V in the LMW, while Cu and Ag were mostly associated with MT. MT isoforms were characterized using the HPLC/ICP-MS. Two and four obvious peaks appeared in the maternal and fetal MT fractions, respectively. The highest elemental ion intensities were at a retention time of 7.8 min for the mother, and for the fetus the peak elemental ion intensities occurred at a retention time of 4.3 min, suggesting that different MT isoforms may be involved in elemental accumulation in maternal and fetal hepatocytosols. (C) 2003 Elsevier Ltd. All rights reserved.
Jian Yang; Takashi Kunito; Yasumi Anan; Shinsuke Tanabe; Nobuyuki Miyazaki.Total and subcellular distribution of trace elements in the liver of a mother-fetus pair of Dall's porpoises (Phocoenoides dalli),MARINE POLLUTION BULLETIN,2004,48(11-12):1122-1129