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Differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers
Wang, Y; Zhou, L; Yao, B; Li, CJ; Gui, JF; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2004-05-31
Source PublicationMOLECULAR AND CELLULAR ENDOCRINOLOGY
ISSN0303-7207
Volume220Issue:1-2Pages:77-88
AbstractWe have cloned and characterized the full-length cDNA encoding thyroid-stimulating hormone beta-subunit (TSHbeta) from orange-spotted grouper Epinephelus coioides. It contains 913 nucleotides with an open reading frame encoding 146 amino acids with a 20 amino acid signal peptide. The grouper mature TSHbeta has 75, 70, 61, 59, 41, 42 and 40% identities to that of rainbow trout, Atlantic salmon, zebrafish, European eel, chicken. mouse and human, respectively. RT-PCR analysis indicated that the TSHbeta mRNA was expressed abundantly not only in pituitary but also in gonads. A more interesting finding is to reveal the differential TSHbeta expressions between the ovaries and the transitional gonads or testes in natural individuals of orange-spotted grouper and red-spotted grouper Epinephelus akaara, and in artificial sex reversal individuals of red-spotted grouper induced by MT feeding. In situ hybridization localization provided direct evidence that the TSHbeta was transcribed in the germ cells. In the growing oocytes, the TSHbeta transcripts were concentrated on the ooplasm periphery. In testicular tissues, the intensively expressed TSHbeta cells were found to be spermatogonia and spermatocytes in the spermatogenic cysts. This is the first report of a TSHbeta expressed in the gonads of any vertebrates in addition to the expected expression in the pituitary, and it expresses more transcripts in the gonads during sex reversal or testis than in the ovaries both in E. coioides and E. akaara. Importantly, the TSHbeta identification in germ cells allows us to further investigate the functional roles and the molecular mechanisms in gametogenesis of groupers, especially in sex reversal and in spermatogenesis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.; We have cloned and characterized the full-length cDNA encoding thyroid-stimulating hormone beta-subunit (TSHbeta) from orange-spotted grouper Epinephelus coioides. It contains 913 nucleotides with an open reading frame encoding 146 amino acids with a 20 amino acid signal peptide. The grouper mature TSHbeta has 75, 70, 61, 59, 41, 42 and 40% identities to that of rainbow trout, Atlantic salmon, zebrafish, European eel, chicken. mouse and human, respectively. RT-PCR analysis indicated that the TSHbeta mRNA was expressed abundantly not only in pituitary but also in gonads. A more interesting finding is to reveal the differential TSHbeta expressions between the ovaries and the transitional gonads or testes in natural individuals of orange-spotted grouper and red-spotted grouper Epinephelus akaara, and in artificial sex reversal individuals of red-spotted grouper induced by MT feeding. In situ hybridization localization provided direct evidence that the TSHbeta was transcribed in the germ cells. In the growing oocytes, the TSHbeta transcripts were concentrated on the ooplasm periphery. In testicular tissues, the intensively expressed TSHbeta cells were found to be spermatogonia and spermatocytes in the spermatogenic cysts. This is the first report of a TSHbeta expressed in the gonads of any vertebrates in addition to the expected expression in the pituitary, and it expresses more transcripts in the gonads during sex reversal or testis than in the ovaries both in E. coioides and E. akaara. Importantly, the TSHbeta identification in germ cells allows us to further investigate the functional roles and the molecular mechanisms in gametogenesis of groupers, especially in sex reversal and in spermatogenesis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
SubtypeArticle
KeywordThyroid-stimulating Hormone Grouper Sex Reversal Spermatogenesis Gonad
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaCell Biology ; Endocrinology & Metabolism
DOI10.1016/j.mce.2004.03.007
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaCell Biology ; Endocrinology & Metabolism
WOS SubjectCell Biology ; Endocrinology & Metabolism
WOS IDWOS:000222570100009
WOS KeywordMOLECULAR-CLONING ; THYROTROPIN RECEPTOR ; GLYCOPROTEIN HORMONES ; EPINEPHELUS-COIOIDES ; THYROCYTE FUNCTION ; CARASSIUS-AURATUS ; SEQUENCE-ANALYSIS ; GENE-EXPRESSION ; TELEOST FISH ; RAT OVARY
Citation statistics
Cited Times:42[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9460
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Y,Zhou, L,Yao, B,et al. Differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2004,220(1-2):77-88.
APA Wang, Y,Zhou, L,Yao, B,Li, CJ,Gui, JF,&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2004).Differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers.MOLECULAR AND CELLULAR ENDOCRINOLOGY,220(1-2),77-88.
MLA Wang, Y,et al."Differential expression of thyroid-stimulating hormone beta subunit in gonads during sex reversal of orange-spotted and red-spotted groupers".MOLECULAR AND CELLULAR ENDOCRINOLOGY 220.1-2(2004):77-88.
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