Natl Inst Hlth, Dept Tropical & Endem Parasit Dis, Seoul 122701, South Korea; Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea; Sungkyunkwan Univ, Sch Med, Ctr Mol Med, Suwon 440746, South Korea; Samsung Biomed Res Inst, Suwon 440746, South Korea; Wuhan Univ, Sch Med, Dept Parasitol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
The diagnostic applicability of the Clonorchis sinensis recombinant 7-kDa protein was evaluated. In enzyme-linked immunosorbent assays and immunoblots, the protein showed high sensitivities (81.3 and 71.9%, respectively) and specificities (92.6 and 89.7%, respectively) for sera obtained from various helminthic infections. Some paragonimiasis sera showed cross-reactions. The antigen might be valuable in the serodiagnosis of human clonorchiasis.