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Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance
Yun Chen; YapingWang; Shunping He and Zuoyan Zhu; Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004
Source PublicationBIOCHEMICAL GENETICS
Volume42Issue:9-10Pages:365-375
AbstractUsing conserved primers and the PCR reaction, the growth hormone (GH) gene and the 3'-UTR of the large yellow croaker (Pseudosciaena crocea) were amplified and sequenced. The gene structure was analyzed and compared to the GH genes of 5 other percoid fish downloaded from Genbank. Also the GH gene of the large yellow croaker and the genes from 14 Percoidei and 2 Labroidei species were aligned using Clustal X. A matrix of 564 bp was used to construct the phylogenetic tree using maximum parsimony and neighbor-joining methods. Phylogenetic trees by the two methods are identical in most of the clades with high bootstrap support. The results are also identical to those from morphological data. In general, this analysis does not support the monophyly of the families Centropomidae and Carangidae. But our GH gene tree indicates that the representative species of the families Sparidae and Sciaenidae are a monophyletic group.
KeywordSciaenidae Perciformes Gh Gene Phylogenetics
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Genetics & Heredity
Indexed BySCI
WOS IDWOS:000223623500006
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9442
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Yun Chen,YapingWang,Shunping He and Zuoyan Zhu,et al. Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance[J]. BIOCHEMICAL GENETICS,2004,42(9-10):365-375.
APA Yun Chen,YapingWang,Shunping He and Zuoyan Zhu,&Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance.BIOCHEMICAL GENETICS,42(9-10),365-375.
MLA Yun Chen,et al."Cloning and sequencing of the growth hormone gene of large yellow croaker and its phylogenetic significance".BIOCHEMICAL GENETICS 42.9-10(2004):365-375.
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