| An ELISA-like time-resolved fluorescence immunoassay for microcystin detection | |
| La-Mei Lei; Ying-Song Wu; Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China | |
| 2004 | |
| Source Publication | CLINICA CHIMICA ACTA
 |
| Volume | 348Issue:1-2Pages:177-180 |
| Abstract | Background: A time-resolved fluorescence immunoassay (TRFIA), based on anti-microcystin-LR (MCLR) monoclonal antibodies (MAbs) and europium-labeled antimouse IgG conjugate, was first developed for microcystin detection. Methods: Anti-MCLR MAbs were prepared by a standard method, and the attained MAbs showed a good cross reactivity with MCLR, MCRR and MCYR. The TRFIA was performed in an indirect competitive mode. The detection method of TRFIA was compared with indirect competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Results: The TRFIA exhibited a typical sigmoidal response for MCLR at concentrations of 0.005-50 ng/ml, with a wide quantitative range between 0.01 and 10 ng/ml, indicating the broadest detective range and the most sensitive of all the methods for microcystins (MCs) detection. Additionally, the TRFIA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (1.6-12.2%). The toxin data of algal samples assayed from TRFIA were in the same range as those with ELISA and HPLC, implying that the method was reliable and practical for the detection of MCs. Conclusions: The TRFIA may offer a valuable alternative or a substitute for conventional ELISA for microcystin detection. (C) 2004 Elsevier B.V. All rights reserved. |
| Keyword | Time-resolved Fluorescence Immunoassay Elisa Microcystin |
| Department | CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| Subject Area | Medical Laboratory Technology |
| Indexed By | SCI |
| WOS ID | WOS:000224337100024 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9406 |
| Collection | 期刊论文 |
| Corresponding Author | Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | La-Mei Lei,Ying-Song Wu,Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China. An ELISA-like time-resolved fluorescence immunoassay for microcystin detection[J]. CLINICA CHIMICA ACTA,2004,348(1-2):177-180. |
| APA | La-Mei Lei,Ying-Song Wu,&Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China.(2004).An ELISA-like time-resolved fluorescence immunoassay for microcystin detection.CLINICA CHIMICA ACTA,348(1-2),177-180. |
| MLA | La-Mei Lei,et al."An ELISA-like time-resolved fluorescence immunoassay for microcystin detection".CLINICA CHIMICA ACTA 348.1-2(2004):177-180. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| An ELISA-like time-r（130KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment