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An ELISA-like time-resolved fluorescence immunoassay for microcystin detection
La-Mei Lei; Ying-Song Wu; Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
2004
Source PublicationCLINICA CHIMICA ACTA
Volume348Issue:1-2Pages:177-180
AbstractBackground: A time-resolved fluorescence immunoassay (TRFIA), based on anti-microcystin-LR (MCLR) monoclonal antibodies (MAbs) and europium-labeled antimouse IgG conjugate, was first developed for microcystin detection. Methods: Anti-MCLR MAbs were prepared by a standard method, and the attained MAbs showed a good cross reactivity with MCLR, MCRR and MCYR. The TRFIA was performed in an indirect competitive mode. The detection method of TRFIA was compared with indirect competitive enzyme-linked immunosorbent assay (ELISA) and high-performance liquid chromatography (HPLC). Results: The TRFIA exhibited a typical sigmoidal response for MCLR at concentrations of 0.005-50 ng/ml, with a wide quantitative range between 0.01 and 10 ng/ml, indicating the broadest detective range and the most sensitive of all the methods for microcystins (MCs) detection. Additionally, the TRFIA maintained good reliability through its quantitative range, as evidenced by low coefficients of variation (1.6-12.2%). The toxin data of algal samples assayed from TRFIA were in the same range as those with ELISA and HPLC, implying that the method was reliable and practical for the detection of MCs. Conclusions: The TRFIA may offer a valuable alternative or a substitute for conventional ELISA for microcystin detection. (C) 2004 Elsevier B.V. All rights reserved.
KeywordTime-resolved Fluorescence Immunoassay Elisa Microcystin
DepartmentCAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Subject AreaMedical Laboratory Technology
Indexed BySCI
WOS IDWOS:000224337100024
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Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9406
Collection期刊论文
Corresponding AuthorSong, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
La-Mei Lei,Ying-Song Wu,Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China. An ELISA-like time-resolved fluorescence immunoassay for microcystin detection[J]. CLINICA CHIMICA ACTA,2004,348(1-2):177-180.
APA La-Mei Lei,Ying-Song Wu,&Song, LR, CAS, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China.(2004).An ELISA-like time-resolved fluorescence immunoassay for microcystin detection.CLINICA CHIMICA ACTA,348(1-2),177-180.
MLA La-Mei Lei,et al."An ELISA-like time-resolved fluorescence immunoassay for microcystin detection".CLINICA CHIMICA ACTA 348.1-2(2004):177-180.
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