Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii | |
Wang, CG; Hu, ZG; Hu, W; Lei, AP; Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China | |
2004-08-01 | |
Source Publication | CHINESE SCIENCE BULLETIN
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ISSN | 1001-6538 |
Volume | 49Issue:16Pages:1713-1717 |
Abstract | The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis. |
Subtype | Article |
Keyword | Transgenic Alga Phbb Gene Poly(3-hydroxybutyrate) (Phb) |
Department | Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China; State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China |
Subject Area | Multidisciplinary Sciences |
DOI | 10.1360/04wc0232 |
WOS Headings | Science & Technology |
Indexed By | SCI |
Language | 英语 |
WOS Research Area | Science & Technology - Other Topics |
WOS Subject | Multidisciplinary Sciences |
WOS ID | WOS:000224549400008 |
WOS Keyword | SELECTABLE MARKER ; POLYHYDROXYALKANOATES ; TRANSFORMATION ; RESISTANCE ; PLANTS |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.ihb.ac.cn/handle/152342/9402 |
Collection | 期刊论文 |
Corresponding Author | Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China |
Affiliation | 1.Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China 2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China |
Recommended Citation GB/T 7714 | Wang, CG,Hu, ZG,Hu, W,et al. Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii[J]. CHINESE SCIENCE BULLETIN,2004,49(16):1713-1717. |
APA | Wang, CG,Hu, ZG,Hu, W,Lei, AP,&Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China.(2004).Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii.CHINESE SCIENCE BULLETIN,49(16),1713-1717. |
MLA | Wang, CG,et al."Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii".CHINESE SCIENCE BULLETIN 49.16(2004):1713-1717. |
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