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学科主题: Multidisciplinary Sciences
题名: Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii
作者: Wang, CG; Hu, ZG; Hu, W; Lei, AP
通讯作者: Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China
关键词: transgenic alga ; phbB gene ; poly(3-hydroxybutyrate) (PHB)
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2004-08-01
DOI: 10.1360/04wc0232
卷: 49, 期:16, 页:1713-1717
收录类别: SCI
文章类型: Article
部门归属: Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China; State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.
英文摘要: The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.
关键词[WOS]: SELECTABLE MARKER ; POLYHYDROXYALKANOATES ; TRANSFORMATION ; RESISTANCE ; PLANTS
语种: 英语
WOS记录号: WOS:000224549400008
ISSN号: 1001-6538
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9402
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China
2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China

Recommended Citation:
.Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii,CHINESE SCIENCE BULLETIN,2004,49(16):1713-1717
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