Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China; State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China
WOS标题词:
Science & Technology
类目[WOS]:
Multidisciplinary Sciences
研究领域[WOS]:
Science & Technology - Other Topics
摘要:
The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.
英文摘要:
The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.
