| Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii | |
| Wang, CG; Hu, ZG; Hu, W; Lei, AP; Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China | |
| 2004-08-01 | |
| Source Publication | CHINESE SCIENCE BULLETIN
 |
| ISSN | 1001-6538 |
| Volume | 49Issue:16Pages:1713-1717 |
| Abstract | The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis. |
| Subtype | Article |
| Keyword | Transgenic Alga Phbb Gene Poly(3-hydroxybutyrate) (Phb) |
| Department | Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China; State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China |
| Subject Area | Multidisciplinary Sciences |
| DOI | 10.1360/04wc0232 |
| WOS Headings | Science & Technology |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000224549400008 |
| WOS Keyword | SELECTABLE MARKER ; POLYHYDROXYALKANOATES ; TRANSFORMATION ; RESISTANCE ; PLANTS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9402 |
| Collection | 期刊论文 |
| Corresponding Author | Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China |
| Affiliation | 1.Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China 2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, CG,Hu, ZG,Hu, W,et al. Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii[J]. CHINESE SCIENCE BULLETIN,2004,49(16):1713-1717. |
| APA | Wang, CG,Hu, ZG,Hu, W,Lei, AP,&Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China.(2004).Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii.CHINESE SCIENCE BULLETIN,49(16),1713-1717. |
| MLA | Wang, CG,et al."Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii".CHINESE SCIENCE BULLETIN 49.16(2004):1713-1717. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Expression and molec（493KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment