IHB OpenIR  > 期刊论文
Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii
Wang, CG; Hu, ZG; Hu, W; Lei, AP; Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China
2004-08-01
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume49Issue:16Pages:1713-1717
AbstractThe expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.; The expression vector containing phbB and ble genes was constructed and transformed into cell-wall-deficient strain Chlamydomonas reinhardtii CC-849 by the glass-head method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mug/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.
SubtypeArticle
KeywordTransgenic Alga Phbb Gene Poly(3-hydroxybutyrate) (Phb)
DepartmentShenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China; State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China
Subject AreaMultidisciplinary Sciences
DOI10.1360/04wc0232
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000224549400008
WOS KeywordSELECTABLE MARKER ; POLYHYDROXYALKANOATES ; TRANSFORMATION ; RESISTANCE ; PLANTS
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9402
Collection期刊论文
Corresponding AuthorHu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China
Affiliation1.Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China
2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Wang, CG,Hu, ZG,Hu, W,et al. Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii[J]. CHINESE SCIENCE BULLETIN,2004,49(16):1713-1717.
APA Wang, CG,Hu, ZG,Hu, W,Lei, AP,&Hu, ZG, Shenzhen Univ, Coll Life Sci, Shenzhen 518060, Peoples R China.(2004).Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii.CHINESE SCIENCE BULLETIN,49(16),1713-1717.
MLA Wang, CG,et al."Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii".CHINESE SCIENCE BULLETIN 49.16(2004):1713-1717.
Files in This Item:
File Name/Size DocType Version Access License
Expression and molec(493KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, CG]'s Articles
[Hu, ZG]'s Articles
[Hu, W]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, CG]'s Articles
[Hu, ZG]'s Articles
[Hu, W]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, CG]'s Articles
[Hu, ZG]'s Articles
[Hu, W]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Expression and molecular analysis of phbB gene in Chlamydomonas reinhardtii.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.