Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips
Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Wuhan Inst Hydrobiol, Wuhan 430071, Peoples R China; Wuhan TB Prevent & Cure Inst, Wuhan, Peoples R China
摘要:
A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing.
Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips.pdf（85KB）
Jiao-Yu Deng; Xian-En Zhang; Hong-Bing Lu; Qiang Liu; Zhi-Ping Zhang; Ya-Feng Zhou; Wei-Hong Xie and Zhi-Jun Fu.Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips,JOURNAL OF CLINICAL MICROBIOLOGY,2004,42(10):4850-4852
Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips.pdf
