| Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips | |
| Jiao-Yu Deng; Xian-En Zhang; Hong-Bing Lu; Qiang Liu; Zhi-Ping Zhang; Ya-Feng Zhou; Wei-Hong Xie and Zhi-Jun Fu; Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China | |
| 2004 | |
| Source Publication | JOURNAL OF CLINICAL MICROBIOLOGY
 |
| Volume | 42Issue:10Pages:4850-4852 |
| Abstract | A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing. |
| Keyword | Polymerase-chain-reaction Line Probe Assay Rapid Detection Drug-resistance Rpob Mutations Gene Strains |
| Department | Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Wuhan Inst Hydrobiol, Wuhan 430071, Peoples R China; Wuhan TB Prevent & Cure Inst, Wuhan, Peoples R China |
| Subject Area | Microbiology |
| Indexed By | SCI |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9400 |
| Collection | 期刊论文 |
| Corresponding Author | Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China |
| Recommended Citation GB/T 7714 | Jiao-Yu Deng,Xian-En Zhang,Hong-Bing Lu,et al. Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2004,42(10):4850-4852. |
| APA | Jiao-Yu Deng.,Xian-En Zhang.,Hong-Bing Lu.,Qiang Liu.,Zhi-Ping Zhang.,...&Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China.(2004).Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips.JOURNAL OF CLINICAL MICROBIOLOGY,42(10),4850-4852. |
| MLA | Jiao-Yu Deng,et al."Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide Ligation assay on DNA chips".JOURNAL OF CLINICAL MICROBIOLOGY 42.10(2004):4850-4852. |
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