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Characterization of development-related genes for the cestode Bothriocephalus acheilognathi
Luo, HY; Nie, P; Chang, MX; Song, Y; Yao, WJ; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2004-10-01
Source PublicationPARASITOLOGY RESEARCH
ISSN0932-0113
Volume94Issue:4Pages:265-274
AbstractDifferential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were up-regulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and that three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.; Differential gene expression of mature and immature Bothriocephalus acheilognathi cestodes was analyzed using the suppression subtractive hybridization technique. Five mature-associated cDNAs were isolated and characterized. Virtual Northern blot and RT-PCR analyses confirmed that four of the five genes were up-regulated in mature parasites. The sequence analysis revealed that one gene encoded the structural protein chorion precursor, and that three encoded functional proteins homologous to yolk ferritin, sodium/hydrogen exchanger and muscin-like protein. Another gene appeared to be specific to B. acheilognathi, encoding a putative metal-bound protein. Although results obtained in the present study are preliminary, the information about the five genes may provide clues for further investigation on the decline in parasite numbers during the maturation of B. acheilognathi.
SubtypeArticle
KeywordMucin-like Protein
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China
Subject AreaParasitology
DOI10.1007/s00436-004-1205-6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaParasitology
WOS SubjectParasitology
WOS IDWOS:000224615200003
WOS KeywordMUCIN-LIKE PROTEIN ; SCHISTOSOMA-MANSONI ; FASCIOLA-HEPATICA ; CYPRINUS-CARPIO ; PRECURSOR ; GLYCOPROTEINS ; SEQUENCES ; PARASITE ; FERRITIN
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9398
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Luo, HY,Nie, P,Chang, MX,et al. Characterization of development-related genes for the cestode Bothriocephalus acheilognathi[J]. PARASITOLOGY RESEARCH,2004,94(4):265-274.
APA Luo, HY,Nie, P,Chang, MX,Song, Y,Yao, WJ,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2004).Characterization of development-related genes for the cestode Bothriocephalus acheilognathi.PARASITOLOGY RESEARCH,94(4),265-274.
MLA Luo, HY,et al."Characterization of development-related genes for the cestode Bothriocephalus acheilognathi".PARASITOLOGY RESEARCH 94.4(2004):265-274.
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