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Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol
Zhong, XP; Xu, Y; Liang, Y; Liao, T; Wang, JW; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2004-03-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
ISSN1532-0456
Volume137Issue:3Pages:291-298
AbstractRare minnow (Gobiocypris rarus) is a tiny Chinese carp that has a short life cycle and is easily cultured in the laboratory. In this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (DES) at 0.05, 0.5 and 5 mug/l in laboratory aquaria. After exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (Vtg) was measured in whole body homogenates. Native and SDS electrophoresis followed by Western blotting were performed for Vtg identification, and a non-competitive ELISA was developed. In the DES exposure groups (0.5 and 5 mug/l DES), Vtg appeared after 4 days, increased significantly after 8 days and reached a maximum on day 13. Further, a significant increase in the hepatosomatic index (HSI) was found in the 5 mug/l DES exposure group after 21 days. These results indicate that rare minnow provides a good model for assessing endocrine disruption by environmental estrogens. (C) 2004 Elsevier Inc. All rights reserved.; Rare minnow (Gobiocypris rarus) is a tiny Chinese carp that has a short life cycle and is easily cultured in the laboratory. In this study, juvenile rare minnows were exposed to waterborne diethylstilbestrol (DES) at 0.05, 0.5 and 5 mug/l in laboratory aquaria. After exposure for 4, 8, 13 and 21 days, juvenile fish were collected and vitellogenin (Vtg) was measured in whole body homogenates. Native and SDS electrophoresis followed by Western blotting were performed for Vtg identification, and a non-competitive ELISA was developed. In the DES exposure groups (0.5 and 5 mug/l DES), Vtg appeared after 4 days, increased significantly after 8 days and reached a maximum on day 13. Further, a significant increase in the hepatosomatic index (HSI) was found in the 5 mug/l DES exposure group after 21 days. These results indicate that rare minnow provides a good model for assessing endocrine disruption by environmental estrogens. (C) 2004 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordDiethylstilbestrol Environmental Estrogens Experimental Animal Hepatosomatic Index Rare Minnow Vitellogenin
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
DOI10.1016/j.cca.2004.02.004
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism ; Toxicology ; Zoology
WOS IDWOS:000224631400008
WOS KeywordMEDAKA ORYZIAS-LATIPES ; PLATICHTHYS-FLESUS ; BIOMARKER ; 17-BETA-ESTRADIOL ; FLOUNDER ; 4-NONYLPHENOL ; PURIFICATION ; PROTEINS ; EXPOSURE ; ASSAY
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9394
Collection期刊论文
Corresponding AuthorXu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhong, XP,Xu, Y,Liang, Y,et al. Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2004,137(3):291-298.
APA Zhong, XP,Xu, Y,Liang, Y,Liao, T,Wang, JW,&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2004).Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,137(3),291-298.
MLA Zhong, XP,et al."Vitellogenin in rare minnow (Gobiocypris rarus): identification and induction by waterborne diethylstilbestrol".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY 137.3(2004):291-298.
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