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学科主题: Biophysics; Biotechnology & Applied Microbiology; Chemistry ; Analytical; Electrochemistry; Nanoscience & Nanotechnology
题名: Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip
作者: Wang, SH; Wen, JK; Zhou, YF; Zhang, ZP; Yang, RF; Zhang, JB; Chen, J; Zhang, XE
通讯作者: Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China
关键词: Bacillus anthracis ; detection ; DNA chip ; multiplex PCR ; anchored primer PCR ; alkaline phosphatase
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2004-11-01
DOI: 10.1016/j.bios.2004.03.019
卷: 20, 期:4, 页:807-813
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
摘要: Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要: Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: POLYMERASE-CHAIN-REACTION ; MOLECULAR CHARACTERIZATION ; CHROMOSOMAL DNA ; SAMPLES ; SPORES ; PXO1
语种: 英语
WOS记录号: WOS:000225412600016
ISSN号: 0956-5663
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9380
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China

Recommended Citation:
Shi-Hua Wang; Ji-Kai Wen; Ya-Feng Zhou; Zhi-Ping Zhang; Rui-Fu Yang; Ji-Bin Zhang; Jia Chen; Xian-En Zhang.Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip,BIOSENSORS & BIOELECTRONICS,2004,20(4):807-813
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