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Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip
Wang, SH; Wen, JK; Zhou, YF; Zhang, ZP; Yang, RF; Zhang, JB; Chen, J; Zhang, XE; Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China
2004-11-01
Source PublicationBIOSENSORS & BIOELECTRONICS
ISSN0956-5663
Volume20Issue:4Pages:807-813
AbstractBacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved.; Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordBacillus Anthracis Detection Dna Chip Multiplex Pcr Anchored Primer Pcr Alkaline Phosphatase
DepartmentChinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
Subject AreaBiophysics ; Analytical ; Biotechnology & Applied Microbiology ; Electrochemistry ; Chemistry ; Nanoscience & Nanotechnology
DOI10.1016/j.bios.2004.03.019
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS SubjectBiophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS IDWOS:000225412600016
WOS KeywordPOLYMERASE-CHAIN-REACTION ; MOLECULAR CHARACTERIZATION ; CHROMOSOMAL DNA ; SAMPLES ; SPORES ; PXO1
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9380
Collection期刊论文
Corresponding AuthorZhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China
Affiliation1.Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China
Recommended Citation
GB/T 7714
Wang, SH,Wen, JK,Zhou, YF,et al. Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip[J]. BIOSENSORS & BIOELECTRONICS,2004,20(4):807-813.
APA Wang, SH.,Wen, JK.,Zhou, YF.,Zhang, ZP.,Yang, RF.,...&Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China.(2004).Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip.BIOSENSORS & BIOELECTRONICS,20(4),807-813.
MLA Wang, SH,et al."Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip".BIOSENSORS & BIOELECTRONICS 20.4(2004):807-813.
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