| Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip | |
| Wang, SH; Wen, JK; Zhou, YF; Zhang, ZP; Yang, RF; Zhang, JB; Chen, J; Zhang, XE; Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China | |
| 2004-11-01 | |
| Source Publication | BIOSENSORS & BIOELECTRONICS
 |
| ISSN | 0956-5663 |
| Volume | 20Issue:4Pages:807-813 |
| Abstract | Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved.; Bacillus anthracis can be identified by detecting virulence factor genes located on two plasmids, pXO1 and pXO2. Combining multiplex PCR with arrayed anchored primer PCR and biotin-avidin alkaline phosphatase indicator system, we developed a qualitative DNA chip method for characterization of B. anthracis, and simultaneous confirmation of the species identity independent of plasmid contents. The assay amplifies pag gene (in pXO1), cap gene (in pXO2) and Ba813 gene (a B. anthracis specific chromosomal marker), and the results were indicated by an easy-to-read profile based on the color reaction of alkaline phosphatase. About 1 pg of specific DNA fragments on the chip wells could be detected after PCR. With the proposed method, the avirulent (pXO1(+)/2(-), pXO1(-)/2(+) and pXO1(-)/2(-)) strains of B. anthracis and distinguished 'anthrax-like' strains from other B. cereus group bacteria were unambiguously identified, while the genera other than Bacillus gave no positive signal. (C) 2004 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Bacillus Anthracis Detection Dna Chip Multiplex Pcr Anchored Primer Pcr Alkaline Phosphatase |
| Department | Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China |
| Subject Area | Biophysics ; Analytical ; Biotechnology & Applied Microbiology ; Electrochemistry ; Chemistry ; Nanoscience & Nanotechnology |
| DOI | 10.1016/j.bios.2004.03.019 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS Subject | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| WOS ID | WOS:000225412600016 |
| WOS Keyword | POLYMERASE-CHAIN-REACTION ; MOLECULAR CHARACTERIZATION ; CHROMOSOMAL DNA ; SAMPLES ; SPORES ; PXO1 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9380 |
| Collection | 期刊论文 |
| Corresponding Author | Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 3.Acad Mil Med Sci, Inst Microbiol & Epidemiol, Beijing 100071, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, SH,Wen, JK,Zhou, YF,et al. Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip[J]. BIOSENSORS & BIOELECTRONICS,2004,20(4):807-813. |
| APA | Wang, SH.,Wen, JK.,Zhou, YF.,Zhang, ZP.,Yang, RF.,...&Zhang, XE, Chinese Acad Sci, Wuhan Inst Virol, Dept Analyt Biochem, Wuhan 430071, Peoples R China.(2004).Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip.BIOSENSORS & BIOELECTRONICS,20(4),807-813. |
| MLA | Wang, SH,et al."Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip".BIOSENSORS & BIOELECTRONICS 20.4(2004):807-813. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Identification and c（160KB） | 开放获取 | -- | View Application Full Text | |||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment