IHB OpenIR  > 期刊论文
Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay
Lin, P; Feng, JJ; Zheng, H; Yang, HH; Xu, JG; Xu, JG, Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China
2005-01-30
Source PublicationTALANTA
ISSN0039-9140
Volume65Issue:2Pages:430-436
AbstractA fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37 C in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Au and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000 ng mL(-1), with detection limits 8 (thermal initiation) and 12 ng mL(1) (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode. (C) 2004 Elsevier B.V. All rights reserved.; A fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37 C in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Au and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000 ng mL(-1), with detection limits 8 (thermal initiation) and 12 ng mL(1) (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode. (C) 2004 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPh-sensitive Phase Separating Polymer Fluorescence Immunoassay Human Igg
DepartmentXiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China; Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China; Jimei Univ, Inst Aquaculture Biotechnol, Xiamen 361021, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaChemistry ; Analytical
DOI10.1016/j.talanta.2004.06.034
WOS HeadingsScience & Technology ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000225381900019
WOS KeywordPHASE-SEPARATING POLYMER ; TIME-RESOLVED FLUOROIMMUNOASSAY ; HYDROGELS ; SYSTEM ; PROTEIN ; IMMOBILIZATION ; DELIVERY ; POINT ; ACID)
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9370
Collection期刊论文
Corresponding AuthorXu, JG, Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China
Affiliation1.Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China
2.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China
3.Jimei Univ, Inst Aquaculture Biotechnol, Xiamen 361021, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Lin, P,Feng, JJ,Zheng, H,et al. Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay[J]. TALANTA,2005,65(2):430-436.
APA Lin, P,Feng, JJ,Zheng, H,Yang, HH,Xu, JG,&Xu, JG, Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China.(2005).Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay.TALANTA,65(2),430-436.
MLA Lin, P,et al."Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay".TALANTA 65.2(2005):430-436.
Files in This Item: Download All
File Name/Size DocType Version Access License
Preparation of pH-se(161KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Lin, P]'s Articles
[Feng, JJ]'s Articles
[Zheng, H]'s Articles
Baidu academic
Similar articles in Baidu academic
[Lin, P]'s Articles
[Feng, JJ]'s Articles
[Zheng, H]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Lin, P]'s Articles
[Feng, JJ]'s Articles
[Zheng, H]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.