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学科主题: Chemistry ; Analytical
题名: Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay
作者: Lin, P; Feng, JJ; Zheng, H; Yang, HH; Xu, JG
通讯作者: Xu, JG, Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China
关键词: pH-sensitive phase separating polymer ; fluorescence immunoassay ; human IgG
刊名: TALANTA
发表日期: 2005-01-30
DOI: 10.1016/j.talanta.2004.06.034
卷: 65, 期:2, 页:430-436
收录类别: SCI
文章类型: Article
部门归属: Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China; Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China; Jimei Univ, Inst Aquaculture Biotechnol, Xiamen 361021, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
摘要: A fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37 C in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Au and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000 ng mL(-1), with detection limits 8 (thermal initiation) and 12 ng mL(1) (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要: A fluorescence immunoassay for human IgG (Ag) was developed using a pH-sensitive polymer prepared by thermal initiation or redox initiation polymerization as a carrier. In the competitive immunoassay, appropriate quantity of Ag was immobilized on the polymer and the standard Ag (or sample) solution, and a constant amount of fluorescein isothiocyanate labeled goat anti-human IgG antibody (Ab-FITC) was added. Immobilized Ag and the standard (or sample) Ag competed for binding to the Ab-FITC in 37 C in homogeneous format. After changing the pH to separate the polymer-immune complex precipitate, it was re-dissolved and determined by fluorescence method. The results showed that the immobilization efficiency, immunological reaction activities of immobilized Au and phase transition pH range were improved as Ag was immobilized by thermal initiation instead of redox initiation polymerization. Under optimum conditions, the calibration graphs for the Ag in both methods, thermal initiation and redox initiation, were linear over the concentration range of 0.0-1000 ng mL(-1), with detection limits 8 (thermal initiation) and 12 ng mL(1) (redox initiation), respectively. Moreover, some pH-sensitive polymer prepared only in organic solvent or under high temperature could also be used as an immunoreaction carrier by thermal initiation polymerization. Thermal initiation polymerization was a better immobilization mode. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: PHASE-SEPARATING POLYMER ; TIME-RESOLVED FLUOROIMMUNOASSAY ; HYDROGELS ; SYSTEM ; PROTEIN ; IMMOBILIZATION ; DELIVERY ; POINT ; ACID)
语种: 英语
WOS记录号: WOS:000225381900019
ISSN号: 0039-9140
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9370
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Xiamen Univ, Dept Chem, MOE, Key Lab Analyt Sci, Xiamen 361005, Peoples R China
2.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China
3.Jimei Univ, Inst Aquaculture Biotechnol, Xiamen 361021, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Peng Lin; Jian-Jun Feng; Hong Zheng; Huang-Hao Yang; Jin-Gou Xu.Preparation of pH-sensitive polymer by thermal initiation polymerization and its application in fluorescence immunoassay,TALANTA,2005,65(2):430-436
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