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学科主题: Fisheries; Marine & Freshwater Biology
题名: Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)
作者: Mao Weifeng ; Wang Yaping ; Wang Wenbo ; Wu Bo ; Feng Jianxin ; Zhu Zuoyan
通讯作者: Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: sperm-mediated gene transfer ; lactoferrin ; Aeromonas hydrophila ; phagocytosis of kidney cells ; grass carp ; Ctenopharyngodon idellus
刊名: AQUACULTURE
发表日期: 2004
卷: 242, 期:1-4, 页:93-103
收录类别: SCI
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
摘要: Human lactoferrin (hLF) is an iron-binding protein with antimicrobial and immunomodulatory activities. hLF cDNA was transferred into grass carp via electroporated sperm. The production of transgenic fish was as high as 55% tinder the best parameters. 2(11) pulses and 20-min incubation. The expression of the transgene was demonstrated by the detection of hLF mRNA by RT-PCR. We also investigated the response of G(0) transgenic grass carp to Aeromonas hydrophila infection. Serum lysozyme activities (P>0.05) and phagocytic activities of kidney cells (P<0.05) were measured in transgenic individuals. The transgenic fish not only cleared A. hydrophila significantly faster than the control carp (P<0.05), but also showed enhanced phagocytic activities. The result shows that hLF has immunomodulatory activities in hLF-transgenic grass carp. The transgenic grass carp exhibited enhanced immunity to A. hydrophila infection. These results reveal that the mechanisms of disease resistance are different between hLF-transgenic plants and hLF-transgenic grass carp. (C) 2004 Elsevier B.V. All rights reserved.
WOS记录号: WOS:000225916000007
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内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9352
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Mao Weifeng; Wang Yaping; Wang Wenbo; Wu Bo; Feng Jianxin; Zhu Zuoyan.Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus),AQUACULTURE,2004,242(1-4):93-103
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