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Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)
Mao Weifeng; Wang Yaping; Wang Wenbo; Wu Bo; Feng Jianxin; Zhu Zuoyan; Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2004
Source PublicationAQUACULTURE
Volume242Issue:1-4Pages:93-103
AbstractHuman lactoferrin (hLF) is an iron-binding protein with antimicrobial and immunomodulatory activities. hLF cDNA was transferred into grass carp via electroporated sperm. The production of transgenic fish was as high as 55% tinder the best parameters. 2(11) pulses and 20-min incubation. The expression of the transgene was demonstrated by the detection of hLF mRNA by RT-PCR. We also investigated the response of G(0) transgenic grass carp to Aeromonas hydrophila infection. Serum lysozyme activities (P>0.05) and phagocytic activities of kidney cells (P<0.05) were measured in transgenic individuals. The transgenic fish not only cleared A. hydrophila significantly faster than the control carp (P<0.05), but also showed enhanced phagocytic activities. The result shows that hLF has immunomodulatory activities in hLF-transgenic grass carp. The transgenic grass carp exhibited enhanced immunity to A. hydrophila infection. These results reveal that the mechanisms of disease resistance are different between hLF-transgenic plants and hLF-transgenic grass carp. (C) 2004 Elsevier B.V. All rights reserved.
KeywordSperm-mediated Gene Transfer Lactoferrin Aeromonas Hydrophila Phagocytosis Of Kidney Cells Grass Carp Ctenopharyngodon Idellus
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
Indexed BySCI
WOS IDWOS:000225916000007
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9352
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Mao Weifeng,Wang Yaping,Wang Wenbo,et al. Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)[J]. AQUACULTURE,2004,242(1-4):93-103.
APA Mao Weifeng.,Wang Yaping.,Wang Wenbo.,Wu Bo.,Feng Jianxin.,...&Zhu, ZY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2004).Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus).AQUACULTURE,242(1-4),93-103.
MLA Mao Weifeng,et al."Enhanced resistance to Aeromonas hydrophila infection and enhanced phagocytic activities in human lactoferrin-transgenic grass carp (Ctenopharyngodon idellus)".AQUACULTURE 242.1-4(2004):93-103.
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