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Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants
Zhou, BS; Liu, WH; Wu, RSS; Lam, PKS; Zhou, BS, City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
2005-01-18
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume71Issue:1Pages:61-72
AbstractA culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved.; A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordGill Epithelia Primary Cell Culture Erod Tilapia b[a]p Pcb Tcdd
DepartmentCity Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/k.aquatox.2004.10.013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000226566400006
WOS KeywordMITOCHONDRIA-RICH CELLS ; O-DEETHYLASE INDUCTION ; DIOXIN-LIKE POLLUTANTS ; WATER RAINBOW-TROUT ; FRESH-WATER ; FUNDULUS-HETEROCLITUS ; BRANCHIAL EPITHELIA ; FISH GILL ; POLYCHLORINATED-BIPHENYLS ; AH RECEPTOR
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9336
Collection期刊论文
Corresponding AuthorZhou, BS, City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
Affiliation1.City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, BS,Liu, WH,Wu, RSS,et al. Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants[J]. AQUATIC TOXICOLOGY,2005,71(1):61-72.
APA Zhou, BS,Liu, WH,Wu, RSS,Lam, PKS,&Zhou, BS, City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China.(2005).Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants.AQUATIC TOXICOLOGY,71(1),61-72.
MLA Zhou, BS,et al."Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants".AQUATIC TOXICOLOGY 71.1(2005):61-72.
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