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学科主题: Marine & Freshwater Biology; Toxicology
题名: Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants
作者: Zhou, BS; Liu, WH; Wu, RSS; Lam, PKS
通讯作者: Zhou, BS, City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
关键词: gill epithelia ; primary cell culture ; EROD ; tilapia ; B[a]P ; PCB ; TCDD
刊名: AQUATIC TOXICOLOGY
发表日期: 2005-01-18
DOI: 10.1016/k.aquatox.2004.10.013
卷: 71, 期:1, 页:61-72
收录类别: SCI
文章类型: Article
部门归属: City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved.
英文摘要: A culture gill epithelium from seawater-adapted tilapia (Oreochromis niloticus) was developed for testing PAHs and dioxin-like contaminants in seawater. The epithelia consists two to three layers of epithelial cells incorporating both pavement cells and mitochondria-rich cells (MRCs). Polarity and a stable transepithelial resistance (TER) were maintained. and closely resembled those in fish gills in vivo. The tightness (integrity) of the epithelia remained unchanged upon exposure to benzo[a]pyrene (B[a]P). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 3,3',4,4',5-pentachlorobiphenyl (PCB#126), while a concentration-dependent response of EROD activity in the epithelia was induced within 18-24 h when the apical side was exposed to these toxicants. The 24 h EC50 of EROD activity was 2.77 x 10(-7) M for PCB#126, 1.85 x 10(-7) M for B[a]P and 7.38 x 10(-10) M for TCDD. showing: that the preparation was not only sensitive to PAHs and dioxin-like compounds, but also able to produce inductive potency of AhR agonists that generally agreed with those derived from other established in vitro and in vivo systems. The results suggest, that the cultured gill epithelia from seawater-adapted tilapia may serve as a simple. rapid and cost-effective tool for assessing exposure and potential effects of toxicants in marine waters. (C) 2004 Elsevier B.V. All rights reserved.
关键词[WOS]: MITOCHONDRIA-RICH CELLS ; O-DEETHYLASE INDUCTION ; DIOXIN-LIKE POLLUTANTS ; WATER RAINBOW-TROUT ; FRESH-WATER ; FUNDULUS-HETEROCLITUS ; BRANCHIAL EPITHELIA ; FISH GILL ; POLYCHLORINATED-BIPHENYLS ; AH RECEPTOR
语种: 英语
WOS记录号: WOS:000226566400006
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9336
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.City Univ Hong Kong, Dept Biol & Chem, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhou, BS; Liu, WH; Wu, RSS; Lam, PKS.Cultured gill epithelial cells from tilapia (Oreochromis niloticus): a new in vitro assay for toxicants,AQUATIC TOXICOLOGY,2005,71(1):61-72
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