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Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay
Wang, JX; Xie, P; Kettrup, A; Schramm, KW; Schramm, KW, GSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany
2005-04-01
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
ISSN1618-2642
Volume381Issue:8Pages:1609-1618
AbstractExposure to indoor air pollution (IAP) from the combustion of biomass fuels is an important cause of morbidity and mortality in developing countries. In the work discussed in this paper we evaluated the endocrine activity of soot particles from biomass fuels by using yeast bioassay. These pollutants could have beta-galactosidase activity with a relative potency (RP) about 10(-7)-10(-9) that of estradiol. Soot particles from wood and straw combustion only partially induced beta-galactosidase activity whereas others produced fully inductive activity in the yeast assay system. These pollutants did not have estrogen antagonist and progesterone agonist activity within the defined concentration range. However, these pollutants require 2-4 orders of magnitude higher IC50 to inhibit the activity of progesterone in a similar dose-response manner to mifepristone. We therefore propose that the endocrine activity of some environmental pollutants may be because of inhibition of the progesterone receptor (hPR). GC-MS results showed that substituted polycyclic aromatic hydrocarbon (PAH) compounds, substituted phenolic compounds and derivatives, aromatic carbonyl compounds, and phytosteroids in these soot particles may be mimicking endogenous hormones.; Exposure to indoor air pollution (IAP) from the combustion of biomass fuels is an important cause of morbidity and mortality in developing countries. In the work discussed in this paper we evaluated the endocrine activity of soot particles from biomass fuels by using yeast bioassay. These pollutants could have beta-galactosidase activity with a relative potency (RP) about 10(-7)-10(-9) that of estradiol. Soot particles from wood and straw combustion only partially induced beta-galactosidase activity whereas others produced fully inductive activity in the yeast assay system. These pollutants did not have estrogen antagonist and progesterone agonist activity within the defined concentration range. However, these pollutants require 2-4 orders of magnitude higher IC50 to inhibit the activity of progesterone in a similar dose-response manner to mifepristone. We therefore propose that the endocrine activity of some environmental pollutants may be because of inhibition of the progesterone receptor (hPR). GC-MS results showed that substituted polycyclic aromatic hydrocarbon (PAH) compounds, substituted phenolic compounds and derivatives, aromatic carbonyl compounds, and phytosteroids in these soot particles may be mimicking endogenous hormones.
SubtypeArticle
KeywordBiomass Fuel Soot Endocrine Modulating Disrupter Yeast Bioassay Reproductive Health
DepartmentGSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; Tech Univ Munich, Lehrstuhl Okol Chem & Umweltanalyt, D-85350 Freising Weihenstephan, Weihenstephan, Germany
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
DOI10.1007/s00216-005-3096-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000228728200019
WOS KeywordPARTICULATE AIR-POLLUTION ; FINE ORGANIC AEROSOL ; ESTROGEN-RECEPTOR ; ENVIRONMENTAL ESTROGENS ; BREAST-CANCER ; WOOD SMOKE ; COMBUSTION ; CHEMICALS ; ASSAY ; PROGESTERONE
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9280
Collection期刊论文
Corresponding AuthorSchramm, KW, GSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany
Affiliation1.GSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
3.Tech Univ Munich, Lehrstuhl Okol Chem & Umweltanalyt, D-85350 Freising Weihenstephan, Weihenstephan, Germany
Recommended Citation
GB/T 7714
Wang, JX,Xie, P,Kettrup, A,et al. Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2005,381(8):1609-1618.
APA Wang, JX,Xie, P,Kettrup, A,Schramm, KW,&Schramm, KW, GSF NAtl Res Ctr Environm & Hlth, Inst Ecol Chem, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany.(2005).Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,381(8),1609-1618.
MLA Wang, JX,et al."Evaluation of soot particles of biomass fuels with endocrine-modulating activity in yeast-based bioassay".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 381.8(2005):1609-1618.
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