| 学科主题: | Genetics & Heredity
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| 题名: | Identification of three duplicated Spin genes in medaka (Oryzias latipes) |
| 作者: | Wang, XL; Mei, J; Sun, M; Hong, YH; Gui, HF
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| 通讯作者: | Gui, HF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
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| 关键词: | Spindlin (Spin)
; gene duplication
; functional divergence
; embryogenesis
|
| 刊名: | GENE
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| 发表日期: | 2005-05-09
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| DOI: | 10.1016/j.gene.2005.02.002
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| 卷: | 350, 期:2, 页:99-106 | | 收录类别: | SCI
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| 文章类型: | Article
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| 部门归属: | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China; Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
|
| WOS标题词: | Science & Technology
; Life Sciences & Biomedicine
|
| 类目[WOS]: | Genetics & Heredity
|
| 研究领域[WOS]: | Genetics & Heredity
|
| 摘要: | Gene and genomic duplications are very important and frequent events in fish evolution, and the divergence of duplicated genes in sequences and functions is a focus of research on gene evolution. Here, we report the identification and characterization of three duplicated Spindlin (Spin) genes from medaka (Oryzias latipes): OlSpinA, OlSpinB, and OlSpinC. Molecular cloning, genomic DNA Blast analysis and phylogenetic relationship analysis demonstrated that the three duplicated OlSpin genes should belong to gene duplication. Furthermore, Western blot analysis revealed significant expression differences of the three OlSpins among different tissues and during embryogenesis in medaka, and suggested that sequence and functional divergence might have occurred in evolution among them. © 2005 Elsevier B.V. All rights reserved. |
| 英文摘要: | Gene and genomic duplications are very important and frequent events in fish evolution, and the divergence of duplicated genes in sequences and functions is a focus of research on gene evolution. Here, we report the identification and characterization of three duplicated Spindlin (Spin) genes from medaka (Oryzias latipes): OlSpinA, OlSpinB, and OlSpinC. Molecular cloning, genomic DNA Blast analysis and phylogenetic relationship analysis demonstrated that the three duplicated OlSpin genes should belong to gene duplication. Furthermore, Western blot analysis revealed significant expression differences of the three OlSpins among different tissues and during embryogenesis in medaka, and suggested that sequence and functional divergence might have occurred in evolution among them. © 2005 Elsevier B.V. All rights reserved. |
| 关键词[WOS]: | INTRON-EXON STRUCTURE
; FISH
; ZEBRAFISH
; CHROMOSOMES
; EVOLUTION
; PRESERVATION
; EXPRESSION
; ORGANISM
; HEDGEHOG
; REVEALS
|
| 语种: | 英语
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| WOS记录号: | WOS:000229559800001
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| ISSN号: | 0378-1119
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| Citation statistics: |
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| 内容类型: | 期刊论文
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| URI标识: | http://ir.ihb.ac.cn/handle/152342/9252
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| Appears in Collections: | 中科院水生所知识产出（2009年前）_期刊论文
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| Identification of three duplicated Spin genes in medaka (Oryzias latipes).pdf（227KB） | 期刊论文 | 出版稿 | 开放获取 | | View
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作者单位: | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
2.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
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