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Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis
Zhang, JY; Wu, YS; Wu, HB; Wang, JG; Li, AH; Li, M; Wang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China
2005-04-18
Source PublicationDISEASES OF AQUATIC ORGANISMS
ISSN0177-5103
Volume64Issue:2Pages:121-126
AbstractThe humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed.; The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed.
SubtypeArticle
KeywordGlugea Epinephelusis Microsporidia Epinephelus Akaara Humoral Immune Responses Antigenicity
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China; First Mil Med Univ, Inst Trop Med, Guangzhou 510515, Guangdong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaFisheries ; Veterinary Sciences
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Veterinary Sciences
WOS SubjectFisheries ; Veterinary Sciences
WOS IDWOS:000230065600004
WOS KeywordANGUILLA-JAPONICA TEMMINCK ; HEAD KIDNEY MACROPHAGES ; SCOPHTHALMUS-MAXIMUS L ; PLECOGLOSSUS-ALTIVELIS ; PSEUDOPLEURONECTES-AMERICANUS ; WINTER FLOUNDER ; SPORES ; PROTOZOA ; AYU ; PHAGOCYTOSIS
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Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9228
Collection期刊论文
Corresponding AuthorWang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China
2.First Mil Med Univ, Inst Trop Med, Guangzhou 510515, Guangdong, Peoples R China
3.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, JY,Wu, YS,Wu, HB,et al. Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis[J]. DISEASES OF AQUATIC ORGANISMS,2005,64(2):121-126.
APA Zhang, JY.,Wu, YS.,Wu, HB.,Wang, JG.,Li, AH.,...&Wang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China.(2005).Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis.DISEASES OF AQUATIC ORGANISMS,64(2),121-126.
MLA Zhang, JY,et al."Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis".DISEASES OF AQUATIC ORGANISMS 64.2(2005):121-126.
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