| Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis | |
| Zhang, JY; Wu, YS; Wu, HB; Wang, JG; Li, AH; Li, M; Wang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China | |
| 2005-04-18 | |
| Source Publication | DISEASES OF AQUATIC ORGANISMS
 |
| ISSN | 0177-5103 |
| Volume | 64Issue:2Pages:121-126 |
| Abstract | The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed.; The humoral immune responses of grouper Epinephelus akaara to a natural infection with Glugea epinephelusis was studied by ELISA utilizing intact mature spores as the coated antigen. Results showed that a specific humoral immune response was elicited, but the intensity of infection (in terms of the number of cysts) was not related to the antibody level in naturally infected hosts. The differences in the antigenicity of intact mature spores and soluble spore proteins derived from cracked mature spores were also analyzed. Results suggested that similar antigen epitopes existed between the 2 groups. Additionally, antigen component patterns and the distribution of antigen with immunogenicity were investigated by using the western blot and the immunofluorescent antibody technique (IFAT). The new parasitic microsporidium has specific polypeptide patterns comparable to the reported fish microsporidians. The main antigenic substances are concentrated on the surface of spores, and are mostly located on the anterior and posterior end of the spore bodies. Most surface components of the G. epinephelusis spores are soluble, The potential role of the surface components in initiating infection was also discussed. |
| Subtype | Article |
| Keyword | Glugea Epinephelusis Microsporidia Epinephelus Akaara Humoral Immune Responses Antigenicity |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China; First Mil Med Univ, Inst Trop Med, Guangzhou 510515, Guangdong, Peoples R China; Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| Subject Area | Fisheries ; Veterinary Sciences |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Veterinary Sciences |
| WOS Subject | Fisheries ; Veterinary Sciences |
| WOS ID | WOS:000230065600004 |
| WOS Keyword | ANGUILLA-JAPONICA TEMMINCK ; HEAD KIDNEY MACROPHAGES ; SCOPHTHALMUS-MAXIMUS L ; PLECOGLOSSUS-ALTIVELIS ; PSEUDOPLEURONECTES-AMERICANUS ; WINTER FLOUNDER ; SPORES ; PROTOZOA ; AYU ; PHAGOCYTOSIS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9228 |
| Collection | 期刊论文 |
| Corresponding Author | Wang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China 2.First Mil Med Univ, Inst Trop Med, Guangzhou 510515, Guangdong, Peoples R China 3.Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| Recommended Citation GB/T 7714 | Zhang, JY,Wu, YS,Wu, HB,et al. Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis[J]. DISEASES OF AQUATIC ORGANISMS,2005,64(2):121-126. |
| APA | Zhang, JY.,Wu, YS.,Wu, HB.,Wang, JG.,Li, AH.,...&Wang, JG, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hebei, Peoples R China.(2005).Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis.DISEASES OF AQUATIC ORGANISMS,64(2),121-126. |
| MLA | Zhang, JY,et al."Humoral immune responses of the grouper Eninephelus akaara against the microsporidium Glugea epinephelusis".DISEASES OF AQUATIC ORGANISMS 64.2(2005):121-126. |
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