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学科主题: Parasitology
题名: Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis
作者: Li, AH; Kong, Y; Cho, SH; Lee, HW; Na, BK; Pak, JK; Kim, TS
通讯作者: Kim, TS, Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea
关键词: Clonorchis sinensis ; copper/zinc superoxide dismutase ; manganese superoxide dismutase ; cDNA ; genomic DNA ; recombinant enzyme ; helminths
刊名: PARASITOLOGY
发表日期: 2005-06-01
DOI: 10.1017/S003118200400695X
卷: 130, 页:687-697
收录类别: SCI
文章类型: Article
部门归属: Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea; Univ Ulsan, Coll Med, Asan Inst Life Sci, Seoul 138736, South Korea
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Parasitology
研究领域[WOS]: Parasitology
摘要: A copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized. Genes Cu/ZnSOD and MnSOD encode proteins of 16 kDa and 25.4 kDa, respectively. The deduced amino acid sequences of the two genes contained highly conserved residues required for activity and secondary structure formation of Cu/ZnSOD and MnSOD, respectively, and show up to 73.7% and 75.4% identities with their counterparts in other animals. The genomic DNA sequence analysis of Cu/ZnSOD gene revealed this as an intronless gene. Inhibitor studies with purified recombinant Cu/ ZnSOD and MnSOD, both of which were functionally expressed in Escherichia coli, confirmed that they are copper/zinc and manganese-containing SOD, respectively. Immunoblots showed that both C. sinensis Cu/ZnSOD and MnSOD should be antigenic for humans, and both, especially the C. sinensis MnSOD, exhibit extensive cross-reactions with sera of patients infected by other trematodes or cestodes. RT-PCR and SOD activity staining of parasite lysates indicate that there are no significant differences in mRNA level or SOD activity for both species of SOD, indicating cytosolic Cu/ZnSOD and MnSOD might play a comparatively important role in the C. sinensis antioxidant system.
英文摘要: A copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized. Genes Cu/ZnSOD and MnSOD encode proteins of 16 kDa and 25.4 kDa, respectively. The deduced amino acid sequences of the two genes contained highly conserved residues required for activity and secondary structure formation of Cu/ZnSOD and MnSOD, respectively, and show up to 73.7% and 75.4% identities with their counterparts in other animals. The genomic DNA sequence analysis of Cu/ZnSOD gene revealed this as an intronless gene. Inhibitor studies with purified recombinant Cu/ ZnSOD and MnSOD, both of which were functionally expressed in Escherichia coli, confirmed that they are copper/zinc and manganese-containing SOD, respectively. Immunoblots showed that both C. sinensis Cu/ZnSOD and MnSOD should be antigenic for humans, and both, especially the C. sinensis MnSOD, exhibit extensive cross-reactions with sera of patients infected by other trematodes or cestodes. RT-PCR and SOD activity staining of parasite lysates indicate that there are no significant differences in mRNA level or SOD activity for both species of SOD, indicating cytosolic Cu/ZnSOD and MnSOD might play a comparatively important role in the C. sinensis antioxidant system.
关键词[WOS]: SCHISTOSOMA-MANSONI ; ONCHOCERCA-VOLVULUS ; ESCHERICHIA-COLI ; IDENTIFICATION ; EXPRESSION ; PURIFICATION ; LEISHMANIA ; DAMAGE ; CDNAS
语种: 英语
WOS记录号: WOS:000230011600012
ISSN号: 0031-1820
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9226
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea
4.Univ Ulsan, Coll Med, Asan Inst Life Sci, Seoul 138736, South Korea

Recommended Citation:
A. H. LI; Y.KONG; S.H. CHO; H.W. LEE; B.K.NA; J.K. PAK and T. S. KIM.Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis,PARASITOLOGY,2005,130():687-697
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