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Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis
Li, AH; Kong, Y; Cho, SH; Lee, HW; Na, BK; Pak, JK; Kim, TS; Kim, TS, Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea
2005-06-01
Source PublicationPARASITOLOGY
ISSN0031-1820
Volume130Pages:687-697
AbstractA copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized. Genes Cu/ZnSOD and MnSOD encode proteins of 16 kDa and 25.4 kDa, respectively. The deduced amino acid sequences of the two genes contained highly conserved residues required for activity and secondary structure formation of Cu/ZnSOD and MnSOD, respectively, and show up to 73.7% and 75.4% identities with their counterparts in other animals. The genomic DNA sequence analysis of Cu/ZnSOD gene revealed this as an intronless gene. Inhibitor studies with purified recombinant Cu/ ZnSOD and MnSOD, both of which were functionally expressed in Escherichia coli, confirmed that they are copper/zinc and manganese-containing SOD, respectively. Immunoblots showed that both C. sinensis Cu/ZnSOD and MnSOD should be antigenic for humans, and both, especially the C. sinensis MnSOD, exhibit extensive cross-reactions with sera of patients infected by other trematodes or cestodes. RT-PCR and SOD activity staining of parasite lysates indicate that there are no significant differences in mRNA level or SOD activity for both species of SOD, indicating cytosolic Cu/ZnSOD and MnSOD might play a comparatively important role in the C. sinensis antioxidant system.; A copper/zinc superoxide dismutase (Cu/ZnSOD) gene and a manganese superoxide dismutase (MnSOD) gene of the human parasite Clonorchis sinensis have been cloned and their gene products functionally characterized. Genes Cu/ZnSOD and MnSOD encode proteins of 16 kDa and 25.4 kDa, respectively. The deduced amino acid sequences of the two genes contained highly conserved residues required for activity and secondary structure formation of Cu/ZnSOD and MnSOD, respectively, and show up to 73.7% and 75.4% identities with their counterparts in other animals. The genomic DNA sequence analysis of Cu/ZnSOD gene revealed this as an intronless gene. Inhibitor studies with purified recombinant Cu/ ZnSOD and MnSOD, both of which were functionally expressed in Escherichia coli, confirmed that they are copper/zinc and manganese-containing SOD, respectively. Immunoblots showed that both C. sinensis Cu/ZnSOD and MnSOD should be antigenic for humans, and both, especially the C. sinensis MnSOD, exhibit extensive cross-reactions with sera of patients infected by other trematodes or cestodes. RT-PCR and SOD activity staining of parasite lysates indicate that there are no significant differences in mRNA level or SOD activity for both species of SOD, indicating cytosolic Cu/ZnSOD and MnSOD might play a comparatively important role in the C. sinensis antioxidant system.
SubtypeArticle
KeywordClonorchis Sinensis Copper/zinc Superoxide Dismutase Manganese Superoxide Dismutase Cdna Genomic Dna Recombinant Enzyme Helminths
DepartmentNatl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea; Univ Ulsan, Coll Med, Asan Inst Life Sci, Seoul 138736, South Korea
Subject AreaParasitology
DOI10.1017/S003118200400695X
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaParasitology
WOS SubjectParasitology
WOS IDWOS:000230011600012
WOS KeywordSCHISTOSOMA-MANSONI ; ONCHOCERCA-VOLVULUS ; ESCHERICHIA-COLI ; IDENTIFICATION ; EXPRESSION ; PURIFICATION ; LEISHMANIA ; DAMAGE ; CDNAS
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9226
Collection期刊论文
Corresponding AuthorKim, TS, Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea
Affiliation1.Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Sungkyunkwan Univ, Sch Med, Dept Mol Parasitol, Suwon 440746, South Korea
4.Univ Ulsan, Coll Med, Asan Inst Life Sci, Seoul 138736, South Korea
Recommended Citation
GB/T 7714
Li, AH,Kong, Y,Cho, SH,et al. Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis[J]. PARASITOLOGY,2005,130:687-697.
APA Li, AH.,Kong, Y.,Cho, SH.,Lee, HW.,Na, BK.,...&Kim, TS, Natl Inst Hlth, Dept Trop & Endem Parasit Dis, Seoul 122701, South Korea.(2005).Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis.PARASITOLOGY,130,687-697.
MLA Li, AH,et al."Molecular cloning and characterization of the copper/zinc and manganese superoxide dismutase genes from the human parasite Clonorchis sinensis".PARASITOLOGY 130(2005):687-697.
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