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学科主题: Immunology; Infectious Diseases
题名: Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss
作者: Chang, CI; Pleguezuelos, O; Zhang, YA; Zou, J; Secombes, CJ
通讯作者: Secombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2T2, Scotland
关键词: CYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; ANTIBACTERIAL PEPTIDES ; AEROMONAS-SALMONICIDA ; EPITHELIAL-CELLS ; INNATE IMMUNITY ; SEQUENCE ; FISH ; EXPRESSION ; ANTIBIOTICS
刊名: INFECTION AND IMMUNITY
发表日期: 2005-08-01
DOI: 10.1128/IAI.73.8.5053-5064.2005
卷: 73, 期:8, 页:5053-5064
收录类别: SCI
文章类型: Article
部门归属: Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2T2, Scotland; Fisheries Res Inst, Chilung, Taiwan; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Immunology ; Infectious Diseases
研究领域[WOS]: Immunology ; Infectious Diseases
摘要: We report the cloning of a novel antimicrobial peptide gene, termed rtCATH_1, found in the rainbow trout, Oncorhynchus mykiss. The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide. This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups, such as the cysteine-bridged family and the specific amino-acid-rich family. The rtCATH_1 gene comprises four exons, as seen in all known mammalian cathelicidin genes, and several transcription factor binding sites known to be of relevance to host defenses were identified in the 5' flanking region. By Northern blot analysis, the expression of rtCATH_1 was detected in gill, head kidney, and spleen of bacterially challenged fish. Primary cultures of head kidney leukocytes from rainbow trout stimulated with lipopolysaccharide or poly(I (.) C) also expressed riCATH_1. A 36-residue peptide corresponding to the core part of the fish cathelicidin was chemically synthesized and shown to exhibit potent antimicrobial activity and a low hemolytic effect. Thus, rtCATH_1 represents a novel antimicrobial peptide gene belonging to the cathelicidin family and may play an important role in the innate immunity of rainbow trout.
英文摘要: We report the cloning of a novel antimicrobial peptide gene, termed rtCATH_1, found in the rainbow trout, Oncorhynchus mykiss. The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide. This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups, such as the cysteine-bridged family and the specific amino-acid-rich family. The rtCATH_1 gene comprises four exons, as seen in all known mammalian cathelicidin genes, and several transcription factor binding sites known to be of relevance to host defenses were identified in the 5' flanking region. By Northern blot analysis, the expression of rtCATH_1 was detected in gill, head kidney, and spleen of bacterially challenged fish. Primary cultures of head kidney leukocytes from rainbow trout stimulated with lipopolysaccharide or poly(I (.) C) also expressed riCATH_1. A 36-residue peptide corresponding to the core part of the fish cathelicidin was chemically synthesized and shown to exhibit potent antimicrobial activity and a low hemolytic effect. Thus, rtCATH_1 represents a novel antimicrobial peptide gene belonging to the cathelicidin family and may play an important role in the innate immunity of rainbow trout.
关键词[WOS]: CYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; ANTIBACTERIAL PEPTIDES ; AEROMONAS-SALMONICIDA ; EPITHELIAL-CELLS ; INNATE IMMUNITY ; SEQUENCE ; FISH ; EXPRESSION ; ANTIBIOTICS
语种: 英语
WOS记录号: WOS:000230760600066
ISSN号: 0019-9567
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9206
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2T2, Scotland
2.Fisheries Res Inst, Chilung, Taiwan
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Chang, CI; Pleguezuelos, O; Zhang, YA; Zou, J; Secombes, CJ.Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss,INFECTION AND IMMUNITY,2005,73(8):5053-5064
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