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Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss
Chang, CI; Pleguezuelos, O; Zhang, YA; Zou, J; Secombes, CJ; Secombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2T2, Scotland
2005-08-01
Source PublicationINFECTION AND IMMUNITY
ISSN0019-9567
Volume73Issue:8Pages:5053-5064
AbstractWe report the cloning of a novel antimicrobial peptide gene, termed rtCATH_1, found in the rainbow trout, Oncorhynchus mykiss. The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide. This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups, such as the cysteine-bridged family and the specific amino-acid-rich family. The rtCATH_1 gene comprises four exons, as seen in all known mammalian cathelicidin genes, and several transcription factor binding sites known to be of relevance to host defenses were identified in the 5' flanking region. By Northern blot analysis, the expression of rtCATH_1 was detected in gill, head kidney, and spleen of bacterially challenged fish. Primary cultures of head kidney leukocytes from rainbow trout stimulated with lipopolysaccharide or poly(I (.) C) also expressed riCATH_1. A 36-residue peptide corresponding to the core part of the fish cathelicidin was chemically synthesized and shown to exhibit potent antimicrobial activity and a low hemolytic effect. Thus, rtCATH_1 represents a novel antimicrobial peptide gene belonging to the cathelicidin family and may play an important role in the innate immunity of rainbow trout.; We report the cloning of a novel antimicrobial peptide gene, termed rtCATH_1, found in the rainbow trout, Oncorhynchus mykiss. The predicted 216-residue rtCATH_1 prepropeptide consists of three domains: a 22-residue signal peptide, a 128-residue cathelin-like region containing two identifiable cathelicidin family signatures, and a predicted 66-residue C-terminal cationic antimicrobial peptide. This predicted mature peptide was unique in possessing features of different known (mammalian) cathelicidin subgroups, such as the cysteine-bridged family and the specific amino-acid-rich family. The rtCATH_1 gene comprises four exons, as seen in all known mammalian cathelicidin genes, and several transcription factor binding sites known to be of relevance to host defenses were identified in the 5' flanking region. By Northern blot analysis, the expression of rtCATH_1 was detected in gill, head kidney, and spleen of bacterially challenged fish. Primary cultures of head kidney leukocytes from rainbow trout stimulated with lipopolysaccharide or poly(I (.) C) also expressed riCATH_1. A 36-residue peptide corresponding to the core part of the fish cathelicidin was chemically synthesized and shown to exhibit potent antimicrobial activity and a low hemolytic effect. Thus, rtCATH_1 represents a novel antimicrobial peptide gene belonging to the cathelicidin family and may play an important role in the innate immunity of rainbow trout.
SubtypeArticle
KeywordCysteine Proteinase-inhibitor Antimicrobial Peptides Antibacterial Peptides Aeromonas-salmonicida Epithelial-cells Innate Immunity Sequence Fish Expression Antibiotics
DepartmentUniv Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2T2, Scotland; Fisheries Res Inst, Chilung, Taiwan; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaImmunology ; Infectious Diseases
DOI10.1128/IAI.73.8.5053-5064.2005
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Infectious Diseases
WOS SubjectImmunology ; Infectious Diseases
WOS IDWOS:000230760600066
WOS KeywordCYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; ANTIBACTERIAL PEPTIDES ; AEROMONAS-SALMONICIDA ; EPITHELIAL-CELLS ; INNATE IMMUNITY ; SEQUENCE ; FISH ; EXPRESSION ; ANTIBIOTICS
Citation statistics
Cited Times:101[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9206
Collection期刊论文
Corresponding AuthorSecombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2T2, Scotland
Affiliation1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2T2, Scotland
2.Fisheries Res Inst, Chilung, Taiwan
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chang, CI,Pleguezuelos, O,Zhang, YA,et al. Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss[J]. INFECTION AND IMMUNITY,2005,73(8):5053-5064.
APA Chang, CI,Pleguezuelos, O,Zhang, YA,Zou, J,Secombes, CJ,&Secombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2T2, Scotland.(2005).Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss.INFECTION AND IMMUNITY,73(8),5053-5064.
MLA Chang, CI,et al."Identification of a novel cathelicidin gene in the rainbow trout, Oncorhynchus mykiss".INFECTION AND IMMUNITY 73.8(2005):5053-5064.
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