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Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis
Zhang, WJ; Yang, J; Yu, YH; Shen, YF; Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China
2005-07-01
Source PublicationJOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN1066-5234
Volume52Issue:4Pages:356-359
AbstractMicrosatellite DNA has been developed into one of the most popular genetic markers. We have identified and cloned microsatellite loci in the genome of a free-living protozoan Euglena gracilis FACHB-848, using the random amplified microsatellites method (RAMS). The digoxigenin-labelled oligonucleotides(CT)(10) and (GT)(10) served as probes to detect complementary sequences in the randomly amplified polymorphic DNA (RAPD) fingerprints produced by means of Southern blotting. Subsequently, positive RAPD fragments were cloned. From a total of 31 RAPD primer profiles, eight microsatellite loci of E. gracilis were detected and characterized. Further, six sites (i.e. EGMS1, EGMS3, EGMS4, EGMS5, EGMS6, and EGMS7) showed polymorphisms. We found a GT or CT microsatellite every 10.5 kb in the genome of E. gracilis, and similar to animal genomes, the (GT)(n) motif was much more abundant than the (CT)(n) motif. These polymorphic microsatellite DNA will serve as advantageous molecular markers for studying the genetic diversity and molecular ecology of Euglena.; Microsatellite DNA has been developed into one of the most popular genetic markers. We have identified and cloned microsatellite loci in the genome of a free-living protozoan Euglena gracilis FACHB-848, using the random amplified microsatellites method (RAMS). The digoxigenin-labelled oligonucleotides(CT)(10) and (GT)(10) served as probes to detect complementary sequences in the randomly amplified polymorphic DNA (RAPD) fingerprints produced by means of Southern blotting. Subsequently, positive RAPD fragments were cloned. From a total of 31 RAPD primer profiles, eight microsatellite loci of E. gracilis were detected and characterized. Further, six sites (i.e. EGMS1, EGMS3, EGMS4, EGMS5, EGMS6, and EGMS7) showed polymorphisms. We found a GT or CT microsatellite every 10.5 kb in the genome of E. gracilis, and similar to animal genomes, the (GT)(n) motif was much more abundant than the (CT)(n) motif. These polymorphic microsatellite DNA will serve as advantageous molecular markers for studying the genetic diversity and molecular ecology of Euglena.
SubtypeArticle
KeywordMolecular Markers Protozoa Rams Rapd Southern Blot
DepartmentChinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China; Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Subject AreaMicrobiology
DOI10.1111/j.1550-7408.2005.00044.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000230942000009
WOS KeywordLINKAGE MAP ; MARKERS ; REPEATS
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Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9196
Collection期刊论文
Corresponding AuthorYu, YH, Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Zhang, WJ,Yang, J,Yu, YH,et al. Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2005,52(4):356-359.
APA Zhang, WJ,Yang, J,Yu, YH,Shen, YF,&Yu, YH, Chinese Acad Sci, Inst Hydrobiol, Lab Protozoan Taxon & Ecol, Wuhan 430072, Peoples R China.(2005).Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis.JOURNAL OF EUKARYOTIC MICROBIOLOGY,52(4),356-359.
MLA Zhang, WJ,et al."Identification, cloning, and characterization of microsatellite DNA in Euglena gracilis".JOURNAL OF EUKARYOTIC MICROBIOLOGY 52.4(2005):356-359.
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