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学科主题: Biotechnology & Applied Microbiology; Virology
题名: Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
作者: Wu, D; Deng, F; Sun, XL; Wang, HL; Yuan, L; Vlak, JM; Hu, ZH
通讯作者: Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China
关键词: NUCLEAR POLYHEDROSIS-VIRUS
刊名: JOURNAL OF GENERAL VIROLOGY
发表日期: 2005-09-01
DOI: 10.1099/vir.0.81110-0
卷: 86, 页:2439-2444
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Univ Wageningen & Res Ctr, Dept Virol, NL-6709 PD Wageningen, Netherlands
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Virology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Virology
摘要: The fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein could be detected from the same time with antiserum against FP25K. To study the function of HearNPV fp25k, a recombinant HearNPV (HaBacWD11) with an enhanced green fluorescent protein (GFP) gene replacing the fp25k was constructed using HaBacHZ8, a bacmid of HearNPV that lacks the polyhedrin gene. Growth curve analysis showed that HaBacWD11 produced higher titres of budded viruses (BVs) than its wild-type counterpart HaBacHZ8-GFP. Electron microscopic analysis indicated that at the late stage of infection, the number of intranuclear enveloped nucleocapsids in HaBacWD11-infected cells was much less than that of HaBacHZ8-GFP. A rescue recombinant virus HaBacWD14 was constructed by reintroducing fp25k gene into HaBacWD11. The growth curve and electron microscopic analysis of the rescued recombinant confirmed that the increase of BV yield and the decrease of the virion production in infected cells were the result of fp25k deletion. The expression of membrane fusion protein (Ha133) and ODV-E66 were studied using the FP25K mutants HaBacWD11 and HaBacHZ8-GFP. Unlike FP25K mutants in Autographa californica multicapsid NPV (AcMNPV), which caused an increase in the expression of membrane fusion protein GP64 and a decrease of ODV-E66, no obvious changes at the expression level of Ha133 and ODV-E66 were observed in HearNPV FP25K mutant.
英文摘要: The fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein could be detected from the same time with antiserum against FP25K. To study the function of HearNPV fp25k, a recombinant HearNPV (HaBacWD11) with an enhanced green fluorescent protein (GFP) gene replacing the fp25k was constructed using HaBacHZ8, a bacmid of HearNPV that lacks the polyhedrin gene. Growth curve analysis showed that HaBacWD11 produced higher titres of budded viruses (BVs) than its wild-type counterpart HaBacHZ8-GFP. Electron microscopic analysis indicated that at the late stage of infection, the number of intranuclear enveloped nucleocapsids in HaBacWD11-infected cells was much less than that of HaBacHZ8-GFP. A rescue recombinant virus HaBacWD14 was constructed by reintroducing fp25k gene into HaBacWD11. The growth curve and electron microscopic analysis of the rescued recombinant confirmed that the increase of BV yield and the decrease of the virion production in infected cells were the result of fp25k deletion. The expression of membrane fusion protein (Ha133) and ODV-E66 were studied using the FP25K mutants HaBacWD11 and HaBacHZ8-GFP. Unlike FP25K mutants in Autographa californica multicapsid NPV (AcMNPV), which caused an increase in the expression of membrane fusion protein GP64 and a decrease of ODV-E66, no obvious changes at the expression level of Ha133 and ODV-E66 were observed in HearNPV FP25K mutant.
关键词[WOS]: NUCLEAR POLYHEDROSIS-VIRUS ; LYMANTRIA-DISPAR NUCLEOPOLYHEDROVIRUS ; 25K FP GENE ; AUTOGRAPHA-CALIFORNICA ; SERIAL PASSAGE ; BACULOVIRUS ; MUTATIONS ; PROTEIN ; IDENTIFICATION ; TRANSPORT
语种: 英语
WOS记录号: WOS:000231482100005
ISSN号: 0022-1317
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9172
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Univ Wageningen & Res Ctr, Dept Virol, NL-6709 PD Wageningen, Netherlands

Recommended Citation:
.Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus,JOURNAL OF GENERAL VIROLOGY,2005,86():2439-2444
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