IHB OpenIR  > 期刊论文
Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus
Wu, D; Deng, F; Sun, XL; Wang, HL; Yuan, L; Vlak, JM; Hu, ZH; Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China
2005-09-01
Source PublicationJOURNAL OF GENERAL VIROLOGY
ISSN0022-1317
Volume86Pages:2439-2444
AbstractThe fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein could be detected from the same time with antiserum against FP25K. To study the function of HearNPV fp25k, a recombinant HearNPV (HaBacWD11) with an enhanced green fluorescent protein (GFP) gene replacing the fp25k was constructed using HaBacHZ8, a bacmid of HearNPV that lacks the polyhedrin gene. Growth curve analysis showed that HaBacWD11 produced higher titres of budded viruses (BVs) than its wild-type counterpart HaBacHZ8-GFP. Electron microscopic analysis indicated that at the late stage of infection, the number of intranuclear enveloped nucleocapsids in HaBacWD11-infected cells was much less than that of HaBacHZ8-GFP. A rescue recombinant virus HaBacWD14 was constructed by reintroducing fp25k gene into HaBacWD11. The growth curve and electron microscopic analysis of the rescued recombinant confirmed that the increase of BV yield and the decrease of the virion production in infected cells were the result of fp25k deletion. The expression of membrane fusion protein (Ha133) and ODV-E66 were studied using the FP25K mutants HaBacWD11 and HaBacHZ8-GFP. Unlike FP25K mutants in Autographa californica multicapsid NPV (AcMNPV), which caused an increase in the expression of membrane fusion protein GP64 and a decrease of ODV-E66, no obvious changes at the expression level of Ha133 and ODV-E66 were observed in HearNPV FP25K mutant.; The fp25k gene of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) was studied. HearNPV fp25k gene transcription was found starting from about 18 h post-infection, and protein could be detected from the same time with antiserum against FP25K. To study the function of HearNPV fp25k, a recombinant HearNPV (HaBacWD11) with an enhanced green fluorescent protein (GFP) gene replacing the fp25k was constructed using HaBacHZ8, a bacmid of HearNPV that lacks the polyhedrin gene. Growth curve analysis showed that HaBacWD11 produced higher titres of budded viruses (BVs) than its wild-type counterpart HaBacHZ8-GFP. Electron microscopic analysis indicated that at the late stage of infection, the number of intranuclear enveloped nucleocapsids in HaBacWD11-infected cells was much less than that of HaBacHZ8-GFP. A rescue recombinant virus HaBacWD14 was constructed by reintroducing fp25k gene into HaBacWD11. The growth curve and electron microscopic analysis of the rescued recombinant confirmed that the increase of BV yield and the decrease of the virion production in infected cells were the result of fp25k deletion. The expression of membrane fusion protein (Ha133) and ODV-E66 were studied using the FP25K mutants HaBacWD11 and HaBacHZ8-GFP. Unlike FP25K mutants in Autographa californica multicapsid NPV (AcMNPV), which caused an increase in the expression of membrane fusion protein GP64 and a decrease of ODV-E66, no obvious changes at the expression level of Ha133 and ODV-E66 were observed in HearNPV FP25K mutant.
SubtypeArticle
KeywordNuclear Polyhedrosis-virus
DepartmentChinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Univ Wageningen & Res Ctr, Dept Virol, NL-6709 PD Wageningen, Netherlands
Subject AreaBiotechnology & Applied Microbiology ; Virology
DOI10.1099/vir.0.81110-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Virology
WOS SubjectBiotechnology & Applied Microbiology ; Virology
WOS IDWOS:000231482100005
WOS KeywordNUCLEAR POLYHEDROSIS-VIRUS ; LYMANTRIA-DISPAR NUCLEOPOLYHEDROVIRUS ; 25K FP GENE ; AUTOGRAPHA-CALIFORNICA ; SERIAL PASSAGE ; BACULOVIRUS ; MUTATIONS ; PROTEIN ; IDENTIFICATION ; TRANSPORT
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9172
Collection期刊论文
Corresponding AuthorHu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China
Affiliation1.Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
4.Univ Wageningen & Res Ctr, Dept Virol, NL-6709 PD Wageningen, Netherlands
Recommended Citation
GB/T 7714
Wu, D,Deng, F,Sun, XL,et al. Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus[J]. JOURNAL OF GENERAL VIROLOGY,2005,86:2439-2444.
APA Wu, D.,Deng, F.,Sun, XL.,Wang, HL.,Yuan, L.,...&Hu, ZH, Chinese Acad Sci, Wuhan Inst Virol, Joint Lab Invertebrate Virol, State Key Lab Virol,Key Lab Mol Virol, Wuhan 430071, Peoples R China.(2005).Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.JOURNAL OF GENERAL VIROLOGY,86,2439-2444.
MLA Wu, D,et al."Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus".JOURNAL OF GENERAL VIROLOGY 86(2005):2439-2444.
Files in This Item:
File Name/Size DocType Version Access License
Functional analysis (277KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wu, D]'s Articles
[Deng, F]'s Articles
[Sun, XL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wu, D]'s Articles
[Deng, F]'s Articles
[Sun, XL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wu, D]'s Articles
[Deng, F]'s Articles
[Sun, XL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Functional analysis of FP25K of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.