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学科主题: Biotechnology & Applied Microbiology; Genetics & Heredity
题名: Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus
作者: Zhi, H; Wang, W; Li, LY; Chai, BF; Sun, YH; Liang, AH
通讯作者: Liang, AH, Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
关键词: Euplotes octocarinatus ; Rab ; cloning ; polymerase chain reaction
刊名: DNA SEQUENCE
发表日期: 2005-08-01
DOI: 10.1080/10425170500087793
卷: 16, 期:4, 页:260-265
收录类别: SCI
文章类型: Article
部门归属: Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]: Biotechnology & Applied Microbiology ; Genetics & Heredity
摘要: Rab proteins belong to the largest family of the Ras superfamily of small GTPase that play an important role in intracellular vesicular traffic. So far, almost 60 members of Rab family have been identified in mammalian cells. To further study the diversity and function of Rab protein in evolution, unicellular protozoa ciliates, Euplotes octocarinatus, were used in this study, Rab genes were screened by PCR method from macronuclear DNA of E. octocarinatus. Sixteen Rab genes were obtained. They share 87.6 - 99.5% identities. Highly conserved GTP-binding domains were found. There are some hot regions that diverse sharply in these genes as well.
英文摘要: Rab proteins belong to the largest family of the Ras superfamily of small GTPase that play an important role in intracellular vesicular traffic. So far, almost 60 members of Rab family have been identified in mammalian cells. To further study the diversity and function of Rab protein in evolution, unicellular protozoa ciliates, Euplotes octocarinatus, were used in this study, Rab genes were screened by PCR method from macronuclear DNA of E. octocarinatus. Sixteen Rab genes were obtained. They share 87.6 - 99.5% identities. Highly conserved GTP-binding domains were found. There are some hot regions that diverse sharply in these genes as well.
关键词[WOS]: BINDING ; GTPASES
语种: 英语
WOS记录号: WOS:000232134200003
ISSN号: 1042-5179
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9142
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhi, H; Wang, W; Li, LY; Chai, BF; Sun, YH; Liang, AH.Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus,DNA SEQUENCE,2005,16(4):260-265
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文件名: Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus.pdf
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