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Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus
Zhi, H; Wang, W; Li, LY; Chai, BF; Sun, YH; Liang, AH; Liang, AH, Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
2005-08-01
Source PublicationDNA SEQUENCE
ISSN1042-5179
Volume16Issue:4Pages:260-265
AbstractRab proteins belong to the largest family of the Ras superfamily of small GTPase that play an important role in intracellular vesicular traffic. So far, almost 60 members of Rab family have been identified in mammalian cells. To further study the diversity and function of Rab protein in evolution, unicellular protozoa ciliates, Euplotes octocarinatus, were used in this study, Rab genes were screened by PCR method from macronuclear DNA of E. octocarinatus. Sixteen Rab genes were obtained. They share 87.6 - 99.5% identities. Highly conserved GTP-binding domains were found. There are some hot regions that diverse sharply in these genes as well.; Rab proteins belong to the largest family of the Ras superfamily of small GTPase that play an important role in intracellular vesicular traffic. So far, almost 60 members of Rab family have been identified in mammalian cells. To further study the diversity and function of Rab protein in evolution, unicellular protozoa ciliates, Euplotes octocarinatus, were used in this study, Rab genes were screened by PCR method from macronuclear DNA of E. octocarinatus. Sixteen Rab genes were obtained. They share 87.6 - 99.5% identities. Highly conserved GTP-binding domains were found. There are some hot regions that diverse sharply in these genes as well.
SubtypeArticle
KeywordEuplotes Octocarinatus Rab Cloning Polymerase Chain Reaction
DepartmentShanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
DOI10.1080/10425170500087793
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS SubjectBiotechnology & Applied Microbiology ; Genetics & Heredity
WOS IDWOS:000232134200003
WOS KeywordBINDING ; GTPASES
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9142
Collection期刊论文
Corresponding AuthorLiang, AH, Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
Affiliation1.Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhi, H,Wang, W,Li, LY,et al. Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus[J]. DNA SEQUENCE,2005,16(4):260-265.
APA Zhi, H.,Wang, W.,Li, LY.,Chai, BF.,Sun, YH.,...&Liang, AH, Shanxi Univ, Inst Biotechnol, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China.(2005).Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus.DNA SEQUENCE,16(4),260-265.
MLA Zhi, H,et al."Cloning and analysis of 16 Rab genes from macronuclear DNA of Euplotes octocarinatus".DNA SEQUENCE 16.4(2005):260-265.
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