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Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)
Cao, XY; Strojsova, A; Znachor, P; Zapomelova, E; Liu, GX; Vrba, J; Zhou, YY; Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2005-08-01
Source PublicationEUROPEAN JOURNAL OF PHYCOLOGY
ISSN0967-0262
Volume40Issue:3Pages:251-258
AbstractThe species-specific production of extracellular phosphatases in phytoplankton of a subtropical polymictic take was investigated from March to May 2004. Phosphatase activity was detected directly at the site of enzyme action using the enzyme-labelled fluorescence (ELF) technique. Size fractionation of bulk phosphatase activity (PA), concentrations of soluble reactive phosphorus (SRP), chlorophyll a, and phytoplankton composition were determined in parallel. Phosphatase-positive cells were present in every phytoplankton sample; labelled cells were detected in 33 algal taxa, including many chlorophytes, dinoflagellates and some diatoms, but never among cyanobacteria. We recorded an unusual dinoflagellate bloom (Peridiniopsis sp.), of which similar to 25% of the cells were phosphatase-positive. Several populations were partly phosphatase-positive whenever present, while some other species never showed any activity. The production of extracellular phosphatases was not primarily regulated by ambient P concentrations; algae produced these enzymes even if SRP concentrations were high. Moreover, heterotrophic nanoflagellates most probably contributed to the pool of particle-bound PA in some samples.; The species-specific production of extracellular phosphatases in phytoplankton of a subtropical polymictic take was investigated from March to May 2004. Phosphatase activity was detected directly at the site of enzyme action using the enzyme-labelled fluorescence (ELF) technique. Size fractionation of bulk phosphatase activity (PA), concentrations of soluble reactive phosphorus (SRP), chlorophyll a, and phytoplankton composition were determined in parallel. Phosphatase-positive cells were present in every phytoplankton sample; labelled cells were detected in 33 algal taxa, including many chlorophytes, dinoflagellates and some diatoms, but never among cyanobacteria. We recorded an unusual dinoflagellate bloom (Peridiniopsis sp.), of which similar to 25% of the cells were phosphatase-positive. Several populations were partly phosphatase-positive whenever present, while some other species never showed any activity. The production of extracellular phosphatases was not primarily regulated by ambient P concentrations; algae produced these enzymes even if SRP concentrations were high. Moreover, heterotrophic nanoflagellates most probably contributed to the pool of particle-bound PA in some samples.
SubtypeArticle
KeywordEnzyme-labelled Fluorescence Phosphatase Activity Phosphorus Phytoplankton Protozoa
DepartmentChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Acad Sci Czech Republ, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic; Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic
Subject AreaPlant Sciences ; Marine & Freshwater Biology
DOI10.1080/09670260500192760
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000232831200002
WOS KeywordDISSOLVED ORGANIC PHOSPHORUS ; ALKALINE-PHOSPHATASE ; WATER ; KINETICS ; SEDIMENTS ; QUANTIFICATION ; AVAILABILITY ; FLUORESCENCE ; DEFICIENCY ; INDICATORS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9120
Collection期刊论文
Corresponding AuthorZhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Acad Sci Czech Republ, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic
4.Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic
Recommended Citation
GB/T 7714
Cao, XY,Strojsova, A,Znachor, P,et al. Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)[J]. EUROPEAN JOURNAL OF PHYCOLOGY,2005,40(3):251-258.
APA Cao, XY.,Strojsova, A.,Znachor, P.,Zapomelova, E.,Liu, GX.,...&Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2005).Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China).EUROPEAN JOURNAL OF PHYCOLOGY,40(3),251-258.
MLA Cao, XY,et al."Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)".EUROPEAN JOURNAL OF PHYCOLOGY 40.3(2005):251-258.
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