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学科主题: Plant Sciences; Marine & Freshwater Biology
题名: Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China)
作者: Cao, XY; Strojsova, A; Znachor, P; Zapomelova, E; Liu, GX; Vrba, J; Zhou, YY
通讯作者: Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: enzyme-labelled fluorescence ; phosphatase activity ; phosphorus ; phytoplankton ; protozoa
刊名: EUROPEAN JOURNAL OF PHYCOLOGY
发表日期: 2005-08-01
DOI: 10.1080/09670260500192760
卷: 40, 期:3, 页:251-258
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; Acad Sci Czech Republ, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic; Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Plant Sciences ; Marine & Freshwater Biology
研究领域[WOS]: Plant Sciences ; Marine & Freshwater Biology
摘要: The species-specific production of extracellular phosphatases in phytoplankton of a subtropical polymictic take was investigated from March to May 2004. Phosphatase activity was detected directly at the site of enzyme action using the enzyme-labelled fluorescence (ELF) technique. Size fractionation of bulk phosphatase activity (PA), concentrations of soluble reactive phosphorus (SRP), chlorophyll a, and phytoplankton composition were determined in parallel. Phosphatase-positive cells were present in every phytoplankton sample; labelled cells were detected in 33 algal taxa, including many chlorophytes, dinoflagellates and some diatoms, but never among cyanobacteria. We recorded an unusual dinoflagellate bloom (Peridiniopsis sp.), of which similar to 25% of the cells were phosphatase-positive. Several populations were partly phosphatase-positive whenever present, while some other species never showed any activity. The production of extracellular phosphatases was not primarily regulated by ambient P concentrations; algae produced these enzymes even if SRP concentrations were high. Moreover, heterotrophic nanoflagellates most probably contributed to the pool of particle-bound PA in some samples.
英文摘要: The species-specific production of extracellular phosphatases in phytoplankton of a subtropical polymictic take was investigated from March to May 2004. Phosphatase activity was detected directly at the site of enzyme action using the enzyme-labelled fluorescence (ELF) technique. Size fractionation of bulk phosphatase activity (PA), concentrations of soluble reactive phosphorus (SRP), chlorophyll a, and phytoplankton composition were determined in parallel. Phosphatase-positive cells were present in every phytoplankton sample; labelled cells were detected in 33 algal taxa, including many chlorophytes, dinoflagellates and some diatoms, but never among cyanobacteria. We recorded an unusual dinoflagellate bloom (Peridiniopsis sp.), of which similar to 25% of the cells were phosphatase-positive. Several populations were partly phosphatase-positive whenever present, while some other species never showed any activity. The production of extracellular phosphatases was not primarily regulated by ambient P concentrations; algae produced these enzymes even if SRP concentrations were high. Moreover, heterotrophic nanoflagellates most probably contributed to the pool of particle-bound PA in some samples.
关键词[WOS]: DISSOLVED ORGANIC PHOSPHORUS ; ALKALINE-PHOSPHATASE ; WATER ; KINETICS ; SEDIMENTS ; QUANTIFICATION ; AVAILABILITY ; FLUORESCENCE ; DEFICIENCY ; INDICATORS
语种: 英语
WOS记录号: WOS:000232831200002
ISSN号: 0967-0262
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9120
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Acad Sci Czech Republ, Inst Hydrobiol, CZ-37005 Ceske Budejovice, Czech Republic
4.Univ S Bohemia, Fac Biol Sci, CZ-37005 Ceske Budejovice, Czech Republic

Recommended Citation:
Cao, XY; Strojsova, A; Znachor, P; Zapomelova, E; Liu, GX; Vrba, J; Zhou, YY.Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China),EUROPEAN JOURNAL OF PHYCOLOGY,2005,40(3):251-258
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