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Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group
Jin, S; Hu, GA; Qian, YH; Zhang, L; Zhang, J; Qiu, G; Zeng, QT; Gui, JF; Gui, JF, Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China
2005-11-01
Source PublicationGENETICA
ISSN0016-6707
Volume125Issue:2-3Pages:223-230
AbstractIntron loss and its evolutionary significance have been noted in Drosophila. The current study provides another example of intron loss within a single-copy Dfak gene in Drosophila. By using polymerase chain reaction (PCR), we amplified about 1.3 kb fragment spanning intron 5-10, located in the position of Tyr kinase (TyK) domain of Dfak gene from Drosophila melanogaster species group, and observed size difference among the amplified DNA fragments from different species. Further sequencing analysis revealed that D. melanogaster and D. simulans deleted an about 60 bp of DNA fragment relative to other 7 Drosophila species, such as D. elegans, D. ficusphila, D. biarmipes, D. takahashii, D. jambulina, D. prostipennis and D. pseudoobscura, and the deleted fragment located precisely in the position of one intron. The data suggested that intron loss might have occurred in the Dfak gene evolutionary process of D. melanogaster and D. simulans of Drosophila melanogaster species group. In addition, the constructed phylogenetic tree based on the Dfak TyK domains clearly revealed the evolutionary relationships between subgroups of Drosophila melanogaster species group, and the intron loss identified from D. melanogaster and D. simulans provides a unique diagnostic tool for taxonomic classification of the melanogaster subgroup from other group of genus Drosophila.; Intron loss and its evolutionary significance have been noted in Drosophila. The current study provides another example of intron loss within a single-copy Dfak gene in Drosophila. By using polymerase chain reaction (PCR), we amplified about 1.3 kb fragment spanning intron 5-10, located in the position of Tyr kinase (TyK) domain of Dfak gene from Drosophila melanogaster species group, and observed size difference among the amplified DNA fragments from different species. Further sequencing analysis revealed that D. melanogaster and D. simulans deleted an about 60 bp of DNA fragment relative to other 7 Drosophila species, such as D. elegans, D. ficusphila, D. biarmipes, D. takahashii, D. jambulina, D. prostipennis and D. pseudoobscura, and the deleted fragment located precisely in the position of one intron. The data suggested that intron loss might have occurred in the Dfak gene evolutionary process of D. melanogaster and D. simulans of Drosophila melanogaster species group. In addition, the constructed phylogenetic tree based on the Dfak TyK domains clearly revealed the evolutionary relationships between subgroups of Drosophila melanogaster species group, and the intron loss identified from D. melanogaster and D. simulans provides a unique diagnostic tool for taxonomic classification of the melanogaster subgroup from other group of genus Drosophila.
SubtypeArticle
KeywordDfak56 Drosophila Melanogaster Intron Loss Phylogenetic Evolution Single-copy Nuclear Gene
DepartmentHubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China; Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Subject AreaGenetics & Heredity
DOI10.1007/s10709-005-8693-x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000232841800010
WOS KeywordFOCAL ADHESION KINASE ; MOLECULAR EVOLUTION ; CARASSIUS-AURATUS ; GAIN ; RNA ; POLYMORPHISM ; TRANSFERRIN ; SEQUENCES ; SELECTION ; INSERTION
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9118
Collection期刊论文
Corresponding AuthorGui, JF, Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China
Affiliation1.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China
2.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, Wuhan Ctr Dev Biol,State Key Lab Freshwater Ecol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Jin, S,Hu, GA,Qian, YH,et al. Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group[J]. GENETICA,2005,125(2-3):223-230.
APA Jin, S.,Hu, GA.,Qian, YH.,Zhang, L.,Zhang, J.,...&Gui, JF, Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China.(2005).Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group.GENETICA,125(2-3),223-230.
MLA Jin, S,et al."Identification of one intron loss and phylogenetic evolution of Dfak gene in the Drosophila melanogaster species group".GENETICA 125.2-3(2005):223-230.
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