| Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles | |
| Song, CL; Cao, XY; Li, JQ; Li, QM; Zhou, YY; Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
| 2005-12-01 | |
| Source Publication | JOURNAL OF FRESHWATER ECOLOGY
 |
| ISSN | 0270-5060 |
| Volume | 20Issue:4Pages:627-634 |
| Abstract | We evaluated the feasibility of microencapsulating dissolved alkaline phosphatase of a water body into reverse micelle systems prepared by hexadecyltrimethylammonium bromide as a surfactant in cyclohexane and 1-butanol as co-surfactant. The dissolved alkaline phosphatase activity within the micelle was described, including its kinetic parameters and the effects of pH and temperature on catalytic activity in surface, overlying and interstitial water of Lake Donghu. We found the similarities on the behavior of dissolved alkaline phosphatase of surface and interstitial water in reverse micelles, which was distinctly different from its behavior in the overlying water. This difference likely reflected the different origins of the dissolved alkaline phosphatase in the vertical profile of the lake. This system provides a novel tool with which to study the diversity and ecological significance of extracellular enzymes in aquatic environments.; We evaluated the feasibility of microencapsulating dissolved alkaline phosphatase of a water body into reverse micelle systems prepared by hexadecyltrimethylammonium bromide as a surfactant in cyclohexane and 1-butanol as co-surfactant. The dissolved alkaline phosphatase activity within the micelle was described, including its kinetic parameters and the effects of pH and temperature on catalytic activity in surface, overlying and interstitial water of Lake Donghu. We found the similarities on the behavior of dissolved alkaline phosphatase of surface and interstitial water in reverse micelles, which was distinctly different from its behavior in the overlying water. This difference likely reflected the different origins of the dissolved alkaline phosphatase in the vertical profile of the lake. This system provides a novel tool with which to study the diversity and ecological significance of extracellular enzymes in aquatic environments. |
| Subtype | Article |
| Keyword | Profiles Phosphorus Enzymes Waters Ocean |
| Department | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Subject Area | Ecology ; Limnology |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| WOS Subject | Ecology ; Limnology |
| WOS ID | WOS:000233141200002 |
| WOS Keyword | PROFILES ; PHOSPHORUS ; ENZYMES ; WATERS ; OCEAN |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9102 |
| Collection | 期刊论文 |
| Corresponding Author | Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Song, CL,Cao, XY,Li, JQ,et al. Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles[J]. JOURNAL OF FRESHWATER ECOLOGY,2005,20(4):627-634. |
| APA | Song, CL,Cao, XY,Li, JQ,Li, QM,Zhou, YY,&Zhou, YY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2005).Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles.JOURNAL OF FRESHWATER ECOLOGY,20(4),627-634. |
| MLA | Song, CL,et al."Vertical variation in dissolved alkaline phosphatase activity in a shallow eutrophic lake determined in reverse micelles".JOURNAL OF FRESHWATER ECOLOGY 20.4(2005):627-634. |
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