| Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides | |
| Zhou, L; Wang, Y; Yao, B; Li, CJ; Ji, GD; Gui, JF; Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2005-12-01 | |
| Source Publication | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| ISSN | 1096-4959 |
| Volume | 142Issue:4Pages:432-437 |
| Abstract | A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. in this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos and maintains a relatively stable expression level during the following embryogenesis. EcApo-14 transcripts are at a very high level during embryonic and early larval development in the yolk syncytial layer (YSL), and decrease in YSL and form intense staining in liver at 3 days after hatching. In adult tissues, EcApo-14 is predominantly expressed in liver and brain. The data suggested that EcApo-14 might play an important role in liver and brain morphogenesis and growth. (c) 2005 Elsevier Inc. All rights reserved.; A novel fish-specific apolipoprotein (apo-14 kDa) has been recently cloned from eel and pufferfish. However, its expression pattern has not been elucidated. in this study, EcApo-14 has been screened from hypothalamic cDNA library of male orange-spotted grouper, which shows 62.9%, 51%, 46.9%, 43.2%, and 31.9% identities to Apo-14 of European flounder, pufferfish, Japanese eel, gibel carp, and grass carp, respectively. RT-PCR analysis reveals that this gene is first transcribed in neurula embryos and maintains a relatively stable expression level during the following embryogenesis. EcApo-14 transcripts are at a very high level during embryonic and early larval development in the yolk syncytial layer (YSL), and decrease in YSL and form intense staining in liver at 3 days after hatching. In adult tissues, EcApo-14 is predominantly expressed in liver and brain. The data suggested that EcApo-14 might play an important role in liver and brain morphogenesis and growth. (c) 2005 Elsevier Inc. All rights reserved. |
| Subtype | Article |
| Keyword | Apolipoproteins Grouper Orange-spotted Grouper Apo-14 Embryogenesis Yolk Syncytial Layer (Ysl) Liver Morphogenesis Expression Pattern Whole-mount In Situ Hybridization |
| Department | Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology ; Zoology |
| DOI | 10.1016/j.cbpb.2005.09.007 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Zoology |
| WOS Subject | Biochemistry & Molecular Biology ; Zoology |
| WOS ID | WOS:000233799400011 |
| WOS Keyword | ZEBRAFISH ; GENE ; LIPOPROTEIN ; METABOLISM ; PROTEIN ; LIVER ; FISH ; PLASMA ; BETA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9080 |
| Collection | 期刊论文 |
| Corresponding Author | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Zhou, L,Wang, Y,Yao, B,et al. Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2005,142(4):432-437. |
| APA | Zhou, L.,Wang, Y.,Yao, B.,Li, CJ.,Ji, GD.,...&Gui, JF, Chinese Acad Sci, Inst Hydrobiol, Wuhun Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2005).Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,142(4),432-437. |
| MLA | Zhou, L,et al."Molecular cloning and expression pattern of 14 kDa apolipoprotein in orange-spotted grouper, Epinephelus coioides".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 142.4(2005):432-437. |
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