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学科主题: Biochemical Research Methods; Chemistry, Analytical
题名: Optimization of RP-HPLC analysis of low molecular weight organic acids in soil
作者: Ding, JH; Wang, XX; Zhang, TL; Li, QM; Luo, MB
通讯作者: Wang, XX, Chinese Acad Sci, Inst Soil Sci, POB X 821,71 E Beijing Rd, Nanjing 20008, Peoples R China
关键词: low molecular weight organic acid ; optimization ; soil solution
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 2006
DOI: 10.1080/10826070500363050
卷: 29, 期:1, 页:99-112
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Soil Sci, Nanjing 20008, Peoples R China; E China Inst Technol, Fuzhou, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; E China Inst Technol, Fuzhou, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
摘要: RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed.
英文摘要: RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed.
关键词[WOS]: VARIABLE CHARGE SOILS ; CHROMATOGRAPHY ; DISSOLUTION ; KAOLINITE ; COMPLEXES ; ANIONS ; PLANTS
语种: 英语
WOS记录号: WOS:000234138700008
ISSN号: 1082-6076
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9072
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Soil Sci, Nanjing 20008, Peoples R China
2.E China Inst Technol, Fuzhou, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
4.E China Inst Technol, Fuzhou, Peoples R China

Recommended Citation:
.Optimization of RP-HPLC analysis of low molecular weight organic acids in soil,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(1):99-112
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