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Optimization of RP-HPLC analysis of low molecular weight organic acids in soil
Ding, JH; Wang, XX; Zhang, TL; Li, QM; Luo, MB; Wang, XX, Chinese Acad Sci, Inst Soil Sci, POB X 821,71 E Beijing Rd, Nanjing 20008, Peoples R China
2006
Source PublicationJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
Volume29Issue:1Pages:99-112
AbstractRP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed.; RP-HPLC analysis for low molecular weight organic acids in soil solution has been optimized. An Atlantis (TM) C-18 column was used for the analyses. An optimal determination for eleven organic acids in soil solution was found at room temperature (25 degrees C) and 220 nm detection wavelength, with a mobile phase of 10 mM KH2PO4 -CH3OH (955, pH 2.7), a flow rate of 0.8 mL/min and 10 mu L sample size. The detection limits ranged 3.2-619 ng/mL, the coefficients of variation ranged 1.3-4.6%, and the recoveries ranged 95.6-106.3% for soil solution with standard addition on the optimal conditions proposed.
SubtypeArticle
KeywordLow Molecular Weight Organic Acid Optimization Soil Solution
Subject AreaBiochemical Research Methods ; Chemistry, Analytical
DepartmentChinese Acad Sci, Inst Soil Sci, Nanjing 20008, Peoples R China; E China Inst Technol, Fuzhou, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; E China Inst Technol, Fuzhou, Peoples R China
DOI10.1080/10826070500363050
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS KeywordVARIABLE CHARGE SOILS ; CHROMATOGRAPHY ; DISSOLUTION ; KAOLINITE ; COMPLEXES ; ANIONS ; PLANTS
WOS IDWOS:000234138700008
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9072
Collection中科院水生所知识产出(2009年前)
Corresponding AuthorWang, XX, Chinese Acad Sci, Inst Soil Sci, POB X 821,71 E Beijing Rd, Nanjing 20008, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Soil Sci, Nanjing 20008, Peoples R China
2.E China Inst Technol, Fuzhou, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
4.E China Inst Technol, Fuzhou, Peoples R China
Recommended Citation
GB/T 7714
Ding, JH,Wang, XX,Zhang, TL,et al. Optimization of RP-HPLC analysis of low molecular weight organic acids in soil[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2006,29(1):99-112.
APA Ding, JH,Wang, XX,Zhang, TL,Li, QM,Luo, MB,&Wang, XX, Chinese Acad Sci, Inst Soil Sci, POB X 821,71 E Beijing Rd, Nanjing 20008, Peoples R China.(2006).Optimization of RP-HPLC analysis of low molecular weight organic acids in soil.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,29(1),99-112.
MLA Ding, JH,et al."Optimization of RP-HPLC analysis of low molecular weight organic acids in soil".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 29.1(2006):99-112.
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