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学科主题: Biochemistry & Molecular Biology; Ecology; Evolutionary Biology
题名: Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis
作者: Zhu, B; Liao, X; Shao, Z; Rosenthal, H; Chang, J
通讯作者: Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China
关键词: Acipenser sinensis ; microsatellites ; Yangtze river
刊名: MOLECULAR ECOLOGY NOTES
发表日期: 2005-12-01
DOI: 10.1111/j.1471-8286.2005.01100.x
卷: 5, 期:4, 页:888-892
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; World Surg Conservat Soc, D-21629 New Wulmstorf, Germany
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
摘要: From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure.
英文摘要: From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure.
语种: 英语
WOS记录号: WOS:000234051200056
ISSN号: 1471-8278
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9068
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.World Surg Conservat Soc, D-21629 New Wulmstorf, Germany

Recommended Citation:
B. ZHU; X. LIAO; Z. SHAO; H. ROSENTHAL and J . CHANG.Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis,MOLECULAR ECOLOGY NOTES,2005,5(4):888-892
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