| Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis | |
| Zhu, B; Liao, X; Shao, Z; Rosenthal, H; Chang, J; Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China | |
| 2005-12-01 | |
| Source Publication | MOLECULAR ECOLOGY NOTES
 |
| ISSN | 1471-8278 |
| Volume | 5Issue:4Pages:888-892 |
| Abstract | From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure.; From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure. |
| Subtype | Article |
| Keyword | Acipenser Sinensis Microsatellites Yangtze River |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; World Surg Conservat Soc, D-21629 New Wulmstorf, Germany |
| Subject Area | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| DOI | 10.1111/j.1471-8286.2005.01100.x |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| WOS ID | WOS:000234051200056 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9068 |
| Collection | 期刊论文 |
| Corresponding Author | Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.World Surg Conservat Soc, D-21629 New Wulmstorf, Germany |
| Recommended Citation GB/T 7714 | Zhu, B,Liao, X,Shao, Z,et al. Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis[J]. MOLECULAR ECOLOGY NOTES,2005,5(4):888-892. |
| APA | Zhu, B,Liao, X,Shao, Z,Rosenthal, H,Chang, J,&Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China.(2005).Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis.MOLECULAR ECOLOGY NOTES,5(4),888-892. |
| MLA | Zhu, B,et al."Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis".MOLECULAR ECOLOGY NOTES 5.4(2005):888-892. |
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