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Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis
Zhu, B; Liao, X; Shao, Z; Rosenthal, H; Chang, J; Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China
2005-12-01
Source PublicationMOLECULAR ECOLOGY NOTES
ISSN1471-8278
Volume5Issue:4Pages:888-892
AbstractFrom (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure.; From (GATA)(n) and (AAAG)(n) enriched genomic libraries for the Chinese sturgeon (Acipenser sinensis), 50 primer pairs were developed using the fast isolation by AFLP of sequences containing repeats (FIASCO) protocol. Forty-six primer pairs exhibited highly polymorphic with two to 11 alleles per locus, while the rest four displayed monomorphic. These markers yielded 246 alleles in a survey of eight specimens of wild A. sinensis. Average observed heterozygosity ranged from 0.13 to 1.00. These loci should provide sufficient levels of genetic diversity to allow parentage analysis for artificial stocking management and delineation of fine-scale population structure.
SubtypeArticle
KeywordAcipenser Sinensis Microsatellites Yangtze River
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; World Surg Conservat Soc, D-21629 New Wulmstorf, Germany
Subject AreaBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
DOI10.1111/j.1471-8286.2005.01100.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000234051200056
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9068
Collection期刊论文
Corresponding AuthorChang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.World Surg Conservat Soc, D-21629 New Wulmstorf, Germany
Recommended Citation
GB/T 7714
Zhu, B,Liao, X,Shao, Z,et al. Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis[J]. MOLECULAR ECOLOGY NOTES,2005,5(4):888-892.
APA Zhu, B,Liao, X,Shao, Z,Rosenthal, H,Chang, J,&Chang, J, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7th So Rd E Lake, Wuhan 430072, Peoples R China.(2005).Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis.MOLECULAR ECOLOGY NOTES,5(4),888-892.
MLA Zhu, B,et al."Isolation and characterization of microsatellites in Chinese sturgeon, Acipenser sinensis".MOLECULAR ECOLOGY NOTES 5.4(2005):888-892.
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