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Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR
Yin, LY; Huang, JQ; Huang, WM; Li, DH; Liu, YD; Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2005-12-15
Source PublicationTOXICON
ISSN0041-0101
Volume46Issue:8Pages:859-864
AbstractMicrocystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved.; Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordMicrocystin-rr Arabidopsis Thaliana Suspension Cells Antioxidant System Malondiadehyde Glutathione Superoxide Dismutase Catalase Glutathione S-transferase
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Wuhan Bot Gargen, Wuhan 430074, Peoples R China; Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430070, Peoples R China
Subject AreaPharmacology & Pharmacy ; Toxicology
DOI10.1016/j.toxicon.2004.12.025
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000234316200004
WOS KeywordGLUTATHIONE S-TRANSFERASES ; CYANOBACTERIAL TOXIN ; SUPEROXIDE-DISMUTASE ; PROTEIN PHOSPHATASES ; AQUATIC ORGANISMS ; SPRAY IRRIGATION ; RAT HEPATOCYTES ; HIGHER-PLANTS ; LR ; GROWTH
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Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9060
Collection期刊论文
Corresponding AuthorLiu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Gargen, Wuhan 430074, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Yin, LY,Huang, JQ,Huang, WM,et al. Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR[J]. TOXICON,2005,46(8):859-864.
APA Yin, LY,Huang, JQ,Huang, WM,Li, DH,Liu, YD,&Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2005).Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR.TOXICON,46(8),859-864.
MLA Yin, LY,et al."Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR".TOXICON 46.8(2005):859-864.
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