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学科主题: Pharmacology & Pharmacy; Toxicology
题名: Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR
作者: Yin, LY; Huang, JQ; Huang, WM; Li, DH; Liu, YD
通讯作者: Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
关键词: microcystin-RR ; Arabidopsis thaliana ; suspension cells ; antioxidant system ; malondiadehyde ; glutathione ; superoxide dismutase ; catalase ; glutathione S-transferase
刊名: TOXICON
发表日期: 2005-12-15
DOI: 10.1016/j.toxicon.2004.12.025
卷: 46, 期:8, 页:859-864
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Wuhan Bot Gargen, Wuhan 430074, Peoples R China; Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
摘要: Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved.
英文摘要: Microcystins are cyclic heptapeptide hepatoxins produced by many species of cyanobacteria. The toxic effects and mechanism of microcystins on animals have been well studied both in vivo and in vitro. It was also reported that microcystins had adverse effects on plants. However, to our knowledge, there is no information about the toxic effects and mechanism of microcystins on plant suspension cells. In this study, Arabidopsis thaliana suspension cells were exposed to a range dose of microcystin-RR. Lipid peroxidation, a main manifestation of oxidative damage, was studied and a time- and dose-dependent increase in malondiadehyde was observed. In contrast, glutathione (GSH) levels in the cells decreased after 48 h treatment with 1 and 5 mg/L of microcystin-RR. The activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after 48 h exposure to I and 5 mg/L of microcystin-RR, but glutathione S-transferase (GST) activity showed no difference compared with the control. These results clearly indicate that microcystin-RR is able to cause oxidative damage in A. thaliana suspension cells. Decrease of GSH content and increases of SOD and CAT activities reveal that the antioxidant system may play an important role in eliminating or alleviating the toxicity of microcystin-RR. The possible toxicity mechanism of microcystin-RR on the A. thaliana suspension cells is also discussed in this paper. (C) 2005 Elsevier Ltd. All rights reserved.
关键词[WOS]: GLUTATHIONE S-TRANSFERASES ; CYANOBACTERIAL TOXIN ; SUPEROXIDE-DISMUTASE ; PROTEIN PHOSPHATASES ; AQUATIC ORGANISMS ; SPRAY IRRIGATION ; RAT HEPATOCYTES ; HIGHER-PLANTS ; LR ; GROWTH
语种: 英语
WOS记录号: WOS:000234316200004
ISSN号: 0041-0101
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9060
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Wuhan Bot Gargen, Wuhan 430074, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430070, Peoples R China

Recommended Citation:
Yin, LY; Huang, JQ; Huang, WM; Li, DH; Liu, YD.Responses of antioxidant system in Arabidopsis thaliana suspension cells to the toxicity of microcystin-RR,TOXICON,2005,46(8):859-864
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