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学科主题: Microbiology; Pharmacology & Pharmacy
题名: Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)
作者: Chang, CI; Zhang, YA; Zou, J; Nie, P; Secombes, CJ
通讯作者: Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2TZ, Scotland
关键词: CYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; IDENTIFICATION ; LEUKOCYTES ; SEQUENCE ; FAMILY ; SERUM ; LIPOPOLYSACCHARIDE ; EXPRESSION ; RESISTANCE
刊名: ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
发表日期: 2006
DOI: 10.1128/AAC.50.1.185-195.2006
卷: 50, 期:1, 页:185-195
收录类别: SCI
文章类型: Article
部门归属: Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland; Res Inst, Chilung, Taiwan; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]: Microbiology ; Pharmacology & Pharmacy
摘要: Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon W. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates.
英文摘要: Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon W. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates.
关键词[WOS]: CYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; IDENTIFICATION ; LEUKOCYTES ; SEQUENCE ; FAMILY ; SERUM ; LIPOPOLYSACCHARIDE ; EXPRESSION ; RESISTANCE
语种: 英语
WOS记录号: WOS:000234988000024
ISSN号: 0066-4804
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9046
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
2.Res Inst, Chilung, Taiwan
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Chang, CI; Zhang, YA; Zou, J; Nie, P; Secombes, CJ.Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar),ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2006,50(1):185-195
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