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Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)
Chang, CI; Zhang, YA; Zou, J; Nie, P; Secombes, CJ; Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2TZ, Scotland
2006
Source PublicationANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN0066-4804
Volume50Issue:1Pages:185-195
AbstractFurther to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon W. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates.; Further to the previous finding of the rainbow trout rtCATH_1 gene, this paper describes three more cathelicidin genes found in salmonids: two in Atlantic salmon, named asCATH_1 and asCATH_2, and one in rainbow trout, named rtCATH_2. All the three new salmonid cathelicidin genes share the common characteristics of mammalian cathelicidin genes, such as consisting of four exons and possessing a highly conserved preproregion and four invariant cysteines clustered in the C-terminal region of the cathelin-like domain. The asCATH_1 gene is homologous to the rainbow trout rtCATH_1 gene, in that it possesses three repeat motifs of TGGGGGTGGC in exon IV and two cysteine residues in the predicted mature peptide, while the asCATH_2 gene and rtCATH_2 gene are homologues of each other, with 96% nucleotide identity. Salmonid cathelicidins possess the same elastase-sensitive residue, threonine, as hagfish cathelicidins and the rabbit CAP18 molecule. The cleavage site of the four salmonid cathelicidins is within a conserved amino acid motif of QKIRTRR, which is at the beginning of the sequence encoded by exon W. Two 36-residue peptides corresponding to the core part of rtCATH_1 and rtCATH_2 were chemically synthesized and shown to exhibit potent antimicrobial activity. rtCATH_2 was expressed constitutively in gill, head kidney, intestine, skin and spleen, while the expression of rtCATH_1 was inducible in gill, head kidney, and spleen after bacterial challenge. Four cathelicidin genes have now been characterized in salmonids and two were identified in hagfish, confirming that cathelicidin genes evolved early and are likely present in all vertebrates.
SubtypeArticle
KeywordCysteine Proteinase-inhibitor Antimicrobial Peptides Identification Leukocytes Sequence Family Serum Lipopolysaccharide Expression Resistance
DepartmentUniv Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland; Res Inst, Chilung, Taiwan; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMicrobiology ; Pharmacology & Pharmacy
DOI10.1128/AAC.50.1.185-195.2006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology ; Pharmacology & Pharmacy
WOS SubjectMicrobiology ; Pharmacology & Pharmacy
WOS IDWOS:000234988000024
WOS KeywordCYSTEINE PROTEINASE-INHIBITOR ; ANTIMICROBIAL PEPTIDES ; IDENTIFICATION ; LEUKOCYTES ; SEQUENCE ; FAMILY ; SERUM ; LIPOPOLYSACCHARIDE ; EXPRESSION ; RESISTANCE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9046
Collection期刊论文
Corresponding AuthorSecombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2TZ, Scotland
Affiliation1.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland
2.Res Inst, Chilung, Taiwan
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chang, CI,Zhang, YA,Zou, J,et al. Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,2006,50(1):185-195.
APA Chang, CI,Zhang, YA,Zou, J,Nie, P,Secombes, CJ,&Secombes, CJ, Univ Aberdeen, Scottish Fish Immunol Res Ctr, Sch Biol Sci, Zool Bldg,Tillydrone Ave, Aberdeen AB24 2TZ, Scotland.(2006).Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar).ANTIMICROBIAL AGENTS AND CHEMOTHERAPY,50(1),185-195.
MLA Chang, CI,et al."Two cathelicidin genes are present in both rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar)".ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 50.1(2006):185-195.
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