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学科主题: Environmental Sciences
题名: Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays
作者: Li, W; Seiffert, M; Xu, Y; Hock, E
通讯作者: Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: ELRA ; YES assay ; estrogenic activity ; tire leachate
刊名: FRESENIUS ENVIRONMENTAL BULLETIN
发表日期: 2006
卷: 15, 期:1, 页:74-79
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Tech Univ Munich, Inst Bot, Wissensch Zentrum Ernahrung Landnutzeng & Umwelt, D-85350 Freising Weihenstephan, Germany
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: In this study, a combination of enzyme-linked receptor assay (ELRA) and yeast estrogen screen (YES) assay was firstly applied to determine whether automobile tires immersed in fresh water can leach chemicals, which display estrogenic activity. We optimized ELRA substituting the chromogene substrate by a luminescent one, and found that luminescent ELBA was more sensitive to 17 beta-estradiol (17 beta-E2) with a detection limit of 0.016 mu g/l, compared to 0.088 mu g/l in the chromogene version. In ELRA, all tire leachates obviously showed estrogenic activity, which was increased with duration of immersion. Moreover, the leachate from hackled tires showed more potent estrogenicity than that from the whole ones. In comparison to ELRA, no detectable estrogenic activity was found in all tire leachates with YES assay. The results from YES assay further evidenced that antiestrogenic compounds can be leached from tires. As tire leachates contain estrogenic compounds, they could be important pollution sources, potentially harmful to wildlife and human health. Thus, use of shredded tires as road fill or in landfill sites should arouse our attention.
英文摘要: In this study, a combination of enzyme-linked receptor assay (ELRA) and yeast estrogen screen (YES) assay was firstly applied to determine whether automobile tires immersed in fresh water can leach chemicals, which display estrogenic activity. We optimized ELRA substituting the chromogene substrate by a luminescent one, and found that luminescent ELBA was more sensitive to 17 beta-estradiol (17 beta-E2) with a detection limit of 0.016 mu g/l, compared to 0.088 mu g/l in the chromogene version. In ELRA, all tire leachates obviously showed estrogenic activity, which was increased with duration of immersion. Moreover, the leachate from hackled tires showed more potent estrogenicity than that from the whole ones. In comparison to ELRA, no detectable estrogenic activity was found in all tire leachates with YES assay. The results from YES assay further evidenced that antiestrogenic compounds can be leached from tires. As tire leachates contain estrogenic compounds, they could be important pollution sources, potentially harmful to wildlife and human health. Thus, use of shredded tires as road fill or in landfill sites should arouse our attention.
关键词[WOS]: ENDOCRINE DISRUPTERS ; CHEMICALS ; ASSAYS ; IDENTIFICATION ; WILDLIFE ; HEALTH
语种: 英语
WOS记录号: WOS:000235286000012
ISSN号: 1018-4619
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9034
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Tech Univ Munich, Inst Bot, Wissensch Zentrum Ernahrung Landnutzeng & Umwelt, D-85350 Freising Weihenstephan, Germany

Recommended Citation:
Li, W; Seiffert, M; Xu, Y; Hock, E.Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays,FRESENIUS ENVIRONMENTAL BULLETIN,2006,15(1):74-79
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