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Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays
Li, W; Seiffert, M; Xu, Y; Hock, E; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2006
Source PublicationFRESENIUS ENVIRONMENTAL BULLETIN
ISSN1018-4619
Volume15Issue:1Pages:74-79
AbstractIn this study, a combination of enzyme-linked receptor assay (ELRA) and yeast estrogen screen (YES) assay was firstly applied to determine whether automobile tires immersed in fresh water can leach chemicals, which display estrogenic activity. We optimized ELRA substituting the chromogene substrate by a luminescent one, and found that luminescent ELBA was more sensitive to 17 beta-estradiol (17 beta-E2) with a detection limit of 0.016 mu g/l, compared to 0.088 mu g/l in the chromogene version. In ELRA, all tire leachates obviously showed estrogenic activity, which was increased with duration of immersion. Moreover, the leachate from hackled tires showed more potent estrogenicity than that from the whole ones. In comparison to ELRA, no detectable estrogenic activity was found in all tire leachates with YES assay. The results from YES assay further evidenced that antiestrogenic compounds can be leached from tires. As tire leachates contain estrogenic compounds, they could be important pollution sources, potentially harmful to wildlife and human health. Thus, use of shredded tires as road fill or in landfill sites should arouse our attention.; In this study, a combination of enzyme-linked receptor assay (ELRA) and yeast estrogen screen (YES) assay was firstly applied to determine whether automobile tires immersed in fresh water can leach chemicals, which display estrogenic activity. We optimized ELRA substituting the chromogene substrate by a luminescent one, and found that luminescent ELBA was more sensitive to 17 beta-estradiol (17 beta-E2) with a detection limit of 0.016 mu g/l, compared to 0.088 mu g/l in the chromogene version. In ELRA, all tire leachates obviously showed estrogenic activity, which was increased with duration of immersion. Moreover, the leachate from hackled tires showed more potent estrogenicity than that from the whole ones. In comparison to ELRA, no detectable estrogenic activity was found in all tire leachates with YES assay. The results from YES assay further evidenced that antiestrogenic compounds can be leached from tires. As tire leachates contain estrogenic compounds, they could be important pollution sources, potentially harmful to wildlife and human health. Thus, use of shredded tires as road fill or in landfill sites should arouse our attention.
SubtypeArticle
KeywordElra Yes Assay Estrogenic Activity Tire Leachate
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Tech Univ Munich, Inst Bot, Wissensch Zentrum Ernahrung Landnutzeng & Umwelt, D-85350 Freising Weihenstephan, Germany
Subject AreaEnvironmental Sciences
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000235286000012
WOS KeywordENDOCRINE DISRUPTERS ; CHEMICALS ; ASSAYS ; IDENTIFICATION ; WILDLIFE ; HEALTH
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9034
Collection期刊论文
Corresponding AuthorXu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Tech Univ Munich, Inst Bot, Wissensch Zentrum Ernahrung Landnutzeng & Umwelt, D-85350 Freising Weihenstephan, Germany
Recommended Citation
GB/T 7714
Li, W,Seiffert, M,Xu, Y,et al. Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2006,15(1):74-79.
APA Li, W,Seiffert, M,Xu, Y,Hock, E,&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays.FRESENIUS ENVIRONMENTAL BULLETIN,15(1),74-79.
MLA Li, W,et al."Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays".FRESENIUS ENVIRONMENTAL BULLETIN 15.1(2006):74-79.
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