| Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays | |
| Li, W; Seiffert, M; Xu, Y; Hock, E; Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2006 | |
| Source Publication | FRESENIUS ENVIRONMENTAL BULLETIN
 |
| ISSN | 1018-4619 |
| Volume | 15Issue:1Pages:74-79 |
| Abstract | In this study, a combination of enzyme-linked receptor assay (ELRA) and yeast estrogen screen (YES) assay was firstly applied to determine whether automobile tires immersed in fresh water can leach chemicals, which display estrogenic activity. We optimized ELRA substituting the chromogene substrate by a luminescent one, and found that luminescent ELBA was more sensitive to 17 beta-estradiol (17 beta-E2) with a detection limit of 0.016 mu g/l, compared to 0.088 mu g/l in the chromogene version. In ELRA, all tire leachates obviously showed estrogenic activity, which was increased with duration of immersion. Moreover, the leachate from hackled tires showed more potent estrogenicity than that from the whole ones. In comparison to ELRA, no detectable estrogenic activity was found in all tire leachates with YES assay. The results from YES assay further evidenced that antiestrogenic compounds can be leached from tires. As tire leachates contain estrogenic compounds, they could be important pollution sources, potentially harmful to wildlife and human health. Thus, use of shredded tires as road fill or in landfill sites should arouse our attention.; In this study, a combination of enzyme-linked receptor assay (ELRA) and yeast estrogen screen (YES) assay was firstly applied to determine whether automobile tires immersed in fresh water can leach chemicals, which display estrogenic activity. We optimized ELRA substituting the chromogene substrate by a luminescent one, and found that luminescent ELBA was more sensitive to 17 beta-estradiol (17 beta-E2) with a detection limit of 0.016 mu g/l, compared to 0.088 mu g/l in the chromogene version. In ELRA, all tire leachates obviously showed estrogenic activity, which was increased with duration of immersion. Moreover, the leachate from hackled tires showed more potent estrogenicity than that from the whole ones. In comparison to ELRA, no detectable estrogenic activity was found in all tire leachates with YES assay. The results from YES assay further evidenced that antiestrogenic compounds can be leached from tires. As tire leachates contain estrogenic compounds, they could be important pollution sources, potentially harmful to wildlife and human health. Thus, use of shredded tires as road fill or in landfill sites should arouse our attention. |
| Subtype | Article |
| Keyword | Elra Yes Assay Estrogenic Activity Tire Leachate |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Tech Univ Munich, Inst Bot, Wissensch Zentrum Ernahrung Landnutzeng & Umwelt, D-85350 Freising Weihenstephan, Germany |
| Subject Area | Environmental Sciences |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Environmental Sciences & Ecology |
| WOS Subject | Environmental Sciences |
| WOS ID | WOS:000235286000012 |
| WOS Keyword | ENDOCRINE DISRUPTERS ; CHEMICALS ; ASSAYS ; IDENTIFICATION ; WILDLIFE ; HEALTH |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/9034 |
| Collection | 期刊论文 |
| Corresponding Author | Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Tech Univ Munich, Inst Bot, Wissensch Zentrum Ernahrung Landnutzeng & Umwelt, D-85350 Freising Weihenstephan, Germany |
| Recommended Citation GB/T 7714 | Li, W,Seiffert, M,Xu, Y,et al. Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2006,15(1):74-79. |
| APA | Li, W,Seiffert, M,Xu, Y,Hock, E,&Xu, Y, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays.FRESENIUS ENVIRONMENTAL BULLETIN,15(1),74-79. |
| MLA | Li, W,et al."Assessment of estrogenic activity of leachate from automobile tires with two in vitro bioassays".FRESENIUS ENVIRONMENTAL BULLETIN 15.1(2006):74-79. |
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