IHB OpenIR  > 期刊论文
Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli
Sun, BJ; Wang, GL; Xie, HX; Gao, Q; Nie, P; Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2006-03-10
Source PublicationAQUACULTURE
ISSN0044-8486
Volume252Issue:2-4Pages:106-113
AbstractA goose-type lysozyme (g-lysozyme) gene has been cloned from the mandarin fish (Siniperca chuatsi), with its recombinant protein expressed in Escherichia coli. From the first transcription initiation site, the mandarin fish g-lysozyme gene extends 1307 nucleotides to the end of the 3' untranslated region, and it contains 5 exons and 4 introns. The open reading frame of the glysozyme transcript has 582 nucleotides which encode a 194 amino acid peptide. The 5' flanking region of mandarin fish glysozyme gene shows several common transcriptional factor binding sites when compared with that from Japanese flounder (Paralichthys olivaceus). The recombinant mandarin fish g-lysozyme was expressed in E. coli by using pET-32a vector, and the purified recombinant g-lysozyme shows lytic activity against Micrococcus lysodeikticus. (c) 2005 Elsevier B.V All rights reserved.; A goose-type lysozyme (g-lysozyme) gene has been cloned from the mandarin fish (Siniperca chuatsi), with its recombinant protein expressed in Escherichia coli. From the first transcription initiation site, the mandarin fish g-lysozyme gene extends 1307 nucleotides to the end of the 3' untranslated region, and it contains 5 exons and 4 introns. The open reading frame of the glysozyme transcript has 582 nucleotides which encode a 194 amino acid peptide. The 5' flanking region of mandarin fish glysozyme gene shows several common transcriptional factor binding sites when compared with that from Japanese flounder (Paralichthys olivaceus). The recombinant mandarin fish g-lysozyme was expressed in E. coli by using pET-32a vector, and the purified recombinant g-lysozyme shows lytic activity against Micrococcus lysodeikticus. (c) 2005 Elsevier B.V All rights reserved.
SubtypeArticle
KeywordG-type Lysozyme Gene Organization Promoter Recombinant Protein Mandarin Fish Chinese Perch Siniperca Chuatsi
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China; NW Sci Tech Univ Agr & Forestry, Coll Anim Sci, Shanxi 712100, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2005.07.046
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000235784200002
WOS KeywordMOLECULAR-CLONING ; CHICKEN-TYPE ; EXPRESSION ; EVOLUTION ; SEQUENCE ; PROTEIN ; SYSTEM ; CDNA ; PROMOTER ; OCT-1
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9028
Collection期刊论文
Corresponding AuthorNie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Lab Fish Dis, Wuhan 430072, Peoples R China
3.NW Sci Tech Univ Agr & Forestry, Coll Anim Sci, Shanxi 712100, Peoples R China
Recommended Citation
GB/T 7714
Sun, BJ,Wang, GL,Xie, HX,et al. Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli[J]. AQUACULTURE,2006,252(2-4):106-113.
APA Sun, BJ,Wang, GL,Xie, HX,Gao, Q,Nie, P,&Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli.AQUACULTURE,252(2-4),106-113.
MLA Sun, BJ,et al."Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli".AQUACULTURE 252.2-4(2006):106-113.
Files in This Item:
File Name/Size DocType Version Access License
Gene structure of go(306KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Sun, BJ]'s Articles
[Wang, GL]'s Articles
[Xie, HX]'s Articles
Baidu academic
Similar articles in Baidu academic
[Sun, BJ]'s Articles
[Wang, GL]'s Articles
[Xie, HX]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Sun, BJ]'s Articles
[Wang, GL]'s Articles
[Xie, HX]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Gene structure of goose-type lysozyme in the mandarin fish Siniperca chuatsi with analysis on the lytic activity of its recombinant in Escherichia coli.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.