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A perspective on fish gonad manipulation for biotechnical applications
Hu, W; Wang, YP; Zhu, ZY; Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2006
Source PublicationCHINESE SCIENCE BULLETIN
ISSN1001-6538
Volume51Issue:1Pages:1-7
AbstractThe gonad is an essential organ for generating sperm and ova in vertebrates. This review describes several pilot studies on gonad gene manipulation and development in fish. With antisense RNA techniques, we suppressed the gonad development, and thus the fertility, of an antisense gonadotropin-releasing hormone (sGnRH) transgenic common carp. Then, using a tissue-specific exogenous gene excision strategy with sexual compensation, we knocked out the gonad-specific transgene. Under the control of the rainbow trout protamine promoter, the transgenic fish expressed the reporter gene eGFP specifically in the spermary. These results indicate that the fish gonad is a new model organ that can improve contemporary biotechnology experiments. Herein we discuss the potential of fish gonad manipulation for resolving important biosafety problems regarding transgenic fish generation and producing the new transgenic animal bioreactor.; The gonad is an essential organ for generating sperm and ova in vertebrates. This review describes several pilot studies on gonad gene manipulation and development in fish. With antisense RNA techniques, we suppressed the gonad development, and thus the fertility, of an antisense gonadotropin-releasing hormone (sGnRH) transgenic common carp. Then, using a tissue-specific exogenous gene excision strategy with sexual compensation, we knocked out the gonad-specific transgene. Under the control of the rainbow trout protamine promoter, the transgenic fish expressed the reporter gene eGFP specifically in the spermary. These results indicate that the fish gonad is a new model organ that can improve contemporary biotechnology experiments. Herein we discuss the potential of fish gonad manipulation for resolving important biosafety problems regarding transgenic fish generation and producing the new transgenic animal bioreactor.
SubtypeReview
KeywordFish Transgene Gonad Manipulation
DepartmentChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaMultidisciplinary Sciences
DOI10.1007/s11434-005-1055-3
WOS HeadingsScience & Technology
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000235676600001
WOS KeywordHIGH-LEVEL EXPRESSION ; T7 RNA-POLYMERASE ; CARP CTENOPHARYNGODON-IDELLUS ; SITE-SPECIFIC RECOMBINATION ; TRANSGENIC SWINE ; ATLANTIC SALMON ; CYPRINUS-CARPIO ; RAINBOW-TROUT ; ANTISENSE RNA ; HORMONE GENE
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Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9026
Collection期刊论文
Corresponding AuthorZhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Hu, W,Wang, YP,Zhu, ZY,et al. A perspective on fish gonad manipulation for biotechnical applications[J]. CHINESE SCIENCE BULLETIN,2006,51(1):1-7.
APA Hu, W,Wang, YP,Zhu, ZY,&Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).A perspective on fish gonad manipulation for biotechnical applications.CHINESE SCIENCE BULLETIN,51(1),1-7.
MLA Hu, W,et al."A perspective on fish gonad manipulation for biotechnical applications".CHINESE SCIENCE BULLETIN 51.1(2006):1-7.
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