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学科主题: Biochemistry & Molecular Biology; Zoology
题名: Identification of a novel C2 domain factor in ovaries of orange-spotted grouper (Epinephelus coioides)
作者: Ji, GD; Zhou, L; Wang, Y; Xia, W; Gui, JF
通讯作者: Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol,Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: grouper ; sex reversal ; follicle ; C2 domain ; EcOC2 factor ; granulosa cell ; oocyte ; oocyte maturation
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
发表日期: 2006-03-01
DOI: 10.1016/j.cbpb.2005.12.012
卷: 143, 期:3, 页:374-383
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Grad Sch, Inst Hydrobiol,Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ; Zoology
摘要: Follicle consists of an oocyte and a lot of surrounding follicular cells, and significant interactions exist between the oocyte and the somatic cells. In this study, a novel cDNA has been screened from a subtractive cDNA library between tail bud embryos and blastula embryos in the protogynous hermaphrodite orange-spotted grouper (Epinephelus coioides). Its full-length cDNA is 821 bp, and has an ORF of 414 by for encoding a peptide of 137 aa, which shows 38%, 37%, 33%, and 33% homology with 4 putative proteins screened from zebrafish (Danio rerio). Conserved domain search in NCBI reveals a single C2 domain existing in the C2 domain superfamily proteins, and has only 7 beta strands in comparison with 8 beta strands of C2 domains in other C2 domain superfamily proteins. Artificial sex reversal, RT-PCR analysis and Western blot detection demonstrated ovary-specific expression of the C2 domain factor, and therefore the novel gene was designated as E. coioides ovary-specific C2 domain factor, EcOC2 factor. Moreover, predominant expression of EcOC2 factor was further revealed in grouper mature ovary, and its strong immunofluorescence signals were located between granulosa cells and oocyte zona radiata in grouper mature follicles. The data indicate that the novel EcOC2 factor might be a main component that associates between granulosa cells and the oocyte during oocyte maturation, and might play significant roles in regulating oocyte maturation and ovulation. Further studies on its developmental behaviour and physiological functions will elucidate the interactions between oocyte and the surrounding somatic cells and the underlying molecular mechanisms. (C) 2005 Elsevier Inc. All rights reserved.
英文摘要: Follicle consists of an oocyte and a lot of surrounding follicular cells, and significant interactions exist between the oocyte and the somatic cells. In this study, a novel cDNA has been screened from a subtractive cDNA library between tail bud embryos and blastula embryos in the protogynous hermaphrodite orange-spotted grouper (Epinephelus coioides). Its full-length cDNA is 821 bp, and has an ORF of 414 by for encoding a peptide of 137 aa, which shows 38%, 37%, 33%, and 33% homology with 4 putative proteins screened from zebrafish (Danio rerio). Conserved domain search in NCBI reveals a single C2 domain existing in the C2 domain superfamily proteins, and has only 7 beta strands in comparison with 8 beta strands of C2 domains in other C2 domain superfamily proteins. Artificial sex reversal, RT-PCR analysis and Western blot detection demonstrated ovary-specific expression of the C2 domain factor, and therefore the novel gene was designated as E. coioides ovary-specific C2 domain factor, EcOC2 factor. Moreover, predominant expression of EcOC2 factor was further revealed in grouper mature ovary, and its strong immunofluorescence signals were located between granulosa cells and oocyte zona radiata in grouper mature follicles. The data indicate that the novel EcOC2 factor might be a main component that associates between granulosa cells and the oocyte during oocyte maturation, and might play significant roles in regulating oocyte maturation and ovulation. Further studies on its developmental behaviour and physiological functions will elucidate the interactions between oocyte and the surrounding somatic cells and the underlying molecular mechanisms. (C) 2005 Elsevier Inc. All rights reserved.
关键词[WOS]: PROTEIN-KINASE-C ; FOLLICULAR DEVELOPMENT ; CRYSTAL-STRUCTURE ; CRUCIAN CARP ; EXPRESSION ; BINDING ; PERFORIN ; BETA ; FERTILIZATION ; SYNAPTOTAGMIN
语种: 英语
WOS记录号: WOS:000236232800016
ISSN号: 1096-4959
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/9012
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol,Wuhan Ctr Dev Biol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Ji, GD; Zhou, L; Wang, Y; Xia, W; Gui, JF.Identification of a novel C2 domain factor in ovaries of orange-spotted grouper (Epinephelus coioides),COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2006,143(3):374-383
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