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NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis
Wang, Q; Wang, YP; Xu, P; Liu, ZJ; Liu, ZJ, Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, 203 Swingle Hall, Auburn, AL 36849 USA
2006-04-01
Source PublicationMOLECULAR IMMUNOLOGY
ISSN0161-5890
Volume43Issue:10Pages:1676-1686
AbstractAntimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In addition to the previously known four classes of antimicrobial peptides, a fifth class of antimicrobial peptides has been recently identified to include NK-lysins that have a globular three-dimensional structure and are larger with 74-78 amino acid residues. NK-lysin has been shown to harbor antimicrobial activities against a wide spectrum of microorganisms including bacteria, fungi, protozoa, and parasites. To date, NK-lysin genes have been reported from only a limited number of organisms. We previously identified a NK-lysin cDNA in channel catfish. Here we report the identification of two noveltypes of NK-lysin transcripts in channel catfish. Altogether, three distinct NK-lysin transcripts exist in channel catfish. In this work, their encoding genes were identified, sequenced, and characterized. We provide strong evidence that the catfish NK-lysin gene is tripled in the same genomic neighborhood. All three catfish NK-lysin genes are present in the same genomic region and are tightly linked on the same chromosome, as the same BAC clones harbor all three copies of the NK-lysin genes. All three NK-lysin genes are expressed, but exhibit distinct expression profiles in various tissues. In spite of the existence of a single copy of NK-lysin gene in the human genome, and only a single hit from the pufferfish,genome, there are two tripled clusters of NK-lysin genes on chromosome 17 of zebrafish in addition to one more copy on its chromosome 5. The similarity in the genomic arrangement of the tripled NK-lysin genes in channel catfish and zebrafish suggest similar evolution of NK-lysin genes. (c) 2005 Elsevier Ltd. All rights reserved.; Antimicrobial peptides (AMPs) are important components of the host innate immune response against microbial invasion. In addition to the previously known four classes of antimicrobial peptides, a fifth class of antimicrobial peptides has been recently identified to include NK-lysins that have a globular three-dimensional structure and are larger with 74-78 amino acid residues. NK-lysin has been shown to harbor antimicrobial activities against a wide spectrum of microorganisms including bacteria, fungi, protozoa, and parasites. To date, NK-lysin genes have been reported from only a limited number of organisms. We previously identified a NK-lysin cDNA in channel catfish. Here we report the identification of two noveltypes of NK-lysin transcripts in channel catfish. Altogether, three distinct NK-lysin transcripts exist in channel catfish. In this work, their encoding genes were identified, sequenced, and characterized. We provide strong evidence that the catfish NK-lysin gene is tripled in the same genomic neighborhood. All three catfish NK-lysin genes are present in the same genomic region and are tightly linked on the same chromosome, as the same BAC clones harbor all three copies of the NK-lysin genes. All three NK-lysin genes are expressed, but exhibit distinct expression profiles in various tissues. In spite of the existence of a single copy of NK-lysin gene in the human genome, and only a single hit from the pufferfish,genome, there are two tripled clusters of NK-lysin genes on chromosome 17 of zebrafish in addition to one more copy on its chromosome 5. The similarity in the genomic arrangement of the tripled NK-lysin genes in channel catfish and zebrafish suggest similar evolution of NK-lysin genes. (c) 2005 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordAntimicrobial Peptide Fish catFish Disease Nk-lysin Innate Immunity Gene
DepartmentAuburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA; Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA; E China Normal Univ, Shanghai 200062, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaBiochemistry & Molecular Biology ; Immunology
DOI10.1016/j.molimm.2005.09.023
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Immunology
WOS SubjectBiochemistry & Molecular Biology ; Immunology
WOS IDWOS:000236450900019
WOS KeywordANTIMICROBIAL PEPTIDE GENE ; T-CELL-ACTIVATION ; ICTALURUS-PUNCTATUS ; WINTER FLOUNDER ; DIFFERENTIAL EXPRESSION ; AMERICANUS WALBAUM ; GENOMIC STRUCTURE ; HEPCIDIN GENE ; CC CHEMOKINES ; TELEOST FISH
Citation statistics
Cited Times:52[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/9004
Collection期刊论文
Corresponding AuthorLiu, ZJ, Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, 203 Swingle Hall, Auburn, AL 36849 USA
Affiliation1.Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, Auburn, AL 36849 USA
2.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA
3.E China Normal Univ, Shanghai 200062, Peoples R China
4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Wang, Q,Wang, YP,Xu, P,et al. NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis[J]. MOLECULAR IMMUNOLOGY,2006,43(10):1676-1686.
APA Wang, Q,Wang, YP,Xu, P,Liu, ZJ,&Liu, ZJ, Auburn Univ, Dept Fisheries & Allied Aquacultures, Fish Mol Genet & Biotechnol Lab, 203 Swingle Hall, Auburn, AL 36849 USA.(2006).NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis.MOLECULAR IMMUNOLOGY,43(10),1676-1686.
MLA Wang, Q,et al."NK-lysin of channel catfish: Gene triplication, sequence variation, and expression analysis".MOLECULAR IMMUNOLOGY 43.10(2006):1676-1686.
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