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Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress
Zhou, BS; Liu, WH; Siu, WHL; O'Toole, D; Lam, PKS; Wu, RSS; Wu, RSS, City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
2006-05-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume77Issue:2Pages:136-142
AbstractToxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ROS). Using duroquinone as a model ROS inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (Cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. In vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 mu M duroquinone for 2 h showed a significant increase in the level of ROS in a dose-dependant manner. Sperm motility was significantly reduced in all exposure groups, but lipid peroxidation (LPO) and DNA strand break (measured by comet assay) were only enhanced at 50 mu M and above. A significant decrease in subsequent hatching rate was recorded in all the exposure groups, despite fertilization rate was not affected. In the in vivo experiment, spermatozoa were collected 24 and 72 h after fish received intra-peritoneal injections of 1.0 and 10 mg kg(-1) body weight duroquinone. DNA damage was clearly evident in spermatozoa of all treatment groups after 72 h exposure, and ROS was significantly enhanced in the high concentration group. LPO however, remained unchanged in both treatment groups. The overall results of both our in vitro and in vivo experiments demonstrated that duroquinone can induce ROS production in spermatozoa, which may impair sperm quality and subsequently reproductive success through oxidative stress. (c) 2006 Elsevier B.V. All rights reserved.; Toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ROS). Using duroquinone as a model ROS inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (Cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. In vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 mu M duroquinone for 2 h showed a significant increase in the level of ROS in a dose-dependant manner. Sperm motility was significantly reduced in all exposure groups, but lipid peroxidation (LPO) and DNA strand break (measured by comet assay) were only enhanced at 50 mu M and above. A significant decrease in subsequent hatching rate was recorded in all the exposure groups, despite fertilization rate was not affected. In the in vivo experiment, spermatozoa were collected 24 and 72 h after fish received intra-peritoneal injections of 1.0 and 10 mg kg(-1) body weight duroquinone. DNA damage was clearly evident in spermatozoa of all treatment groups after 72 h exposure, and ROS was significantly enhanced in the high concentration group. LPO however, remained unchanged in both treatment groups. The overall results of both our in vitro and in vivo experiments demonstrated that duroquinone can induce ROS production in spermatozoa, which may impair sperm quality and subsequently reproductive success through oxidative stress. (c) 2006 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordReactive Oxygen Species (Ros) Spermatozoa Duroquinone Comet Assay Lipid Peroxidation (Lpo) Common Carp
DepartmentCity Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Subject AreaMarine & Freshwater Biology ; Toxicology
DOI10.1016/j.aquatox.2005.11.006
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000236917400003
WOS KeywordOXYGEN SPECIES PRODUCTION ; DNA-DAMAGE ; HUMAN SPERM ; COMET ASSAY ; IN-VITRO ; LIPID-PEROXIDATION ; INDIVIDUAL CELLS ; SEMINAL PLASMA ; MILL EFFLUENT ; WHITE SUCKER
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8994
Collection期刊论文
Corresponding AuthorWu, RSS, City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
Affiliation1.City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, BS,Liu, WH,Siu, WHL,et al. Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress[J]. AQUATIC TOXICOLOGY,2006,77(2):136-142.
APA Zhou, BS.,Liu, WH.,Siu, WHL.,O'Toole, D.,Lam, PKS.,...&Wu, RSS, City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China.(2006).Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress.AQUATIC TOXICOLOGY,77(2),136-142.
MLA Zhou, BS,et al."Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress".AQUATIC TOXICOLOGY 77.2(2006):136-142.
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