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学科主题: Marine & Freshwater Biology; Toxicology
题名: Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress
作者: Zhou, BS; Liu, WH; Siu, WHL; O'Toole, D; Lam, PKS; Wu, RSS
通讯作者: Wu, RSS, City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
关键词: reactive oxygen species (ROS) ; spermatozoa ; duroquinone ; comet assay ; lipid peroxidation (LPO) ; common carp
刊名: AQUATIC TOXICOLOGY
发表日期: 2006-05-01
DOI: 10.1016/j.aquatox.2005.11.006
卷: 77, 期:2, 页:136-142
收录类别: SCI
文章类型: Article
部门归属: City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Marine & Freshwater Biology ; Toxicology
研究领域[WOS]: Marine & Freshwater Biology ; Toxicology
摘要: Toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ROS). Using duroquinone as a model ROS inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (Cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. In vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 mu M duroquinone for 2 h showed a significant increase in the level of ROS in a dose-dependant manner. Sperm motility was significantly reduced in all exposure groups, but lipid peroxidation (LPO) and DNA strand break (measured by comet assay) were only enhanced at 50 mu M and above. A significant decrease in subsequent hatching rate was recorded in all the exposure groups, despite fertilization rate was not affected. In the in vivo experiment, spermatozoa were collected 24 and 72 h after fish received intra-peritoneal injections of 1.0 and 10 mg kg(-1) body weight duroquinone. DNA damage was clearly evident in spermatozoa of all treatment groups after 72 h exposure, and ROS was significantly enhanced in the high concentration group. LPO however, remained unchanged in both treatment groups. The overall results of both our in vitro and in vivo experiments demonstrated that duroquinone can induce ROS production in spermatozoa, which may impair sperm quality and subsequently reproductive success through oxidative stress. (c) 2006 Elsevier B.V. All rights reserved.
英文摘要: Toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ROS). Using duroquinone as a model ROS inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (Cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. In vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 mu M duroquinone for 2 h showed a significant increase in the level of ROS in a dose-dependant manner. Sperm motility was significantly reduced in all exposure groups, but lipid peroxidation (LPO) and DNA strand break (measured by comet assay) were only enhanced at 50 mu M and above. A significant decrease in subsequent hatching rate was recorded in all the exposure groups, despite fertilization rate was not affected. In the in vivo experiment, spermatozoa were collected 24 and 72 h after fish received intra-peritoneal injections of 1.0 and 10 mg kg(-1) body weight duroquinone. DNA damage was clearly evident in spermatozoa of all treatment groups after 72 h exposure, and ROS was significantly enhanced in the high concentration group. LPO however, remained unchanged in both treatment groups. The overall results of both our in vitro and in vivo experiments demonstrated that duroquinone can induce ROS production in spermatozoa, which may impair sperm quality and subsequently reproductive success through oxidative stress. (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: OXYGEN SPECIES PRODUCTION ; DNA-DAMAGE ; HUMAN SPERM ; COMET ASSAY ; IN-VITRO ; LIPID-PEROXIDATION ; INDIVIDUAL CELLS ; SEMINAL PLASMA ; MILL EFFLUENT ; WHITE SUCKER
语种: 英语
WOS记录号: WOS:000236917400003
ISSN号: 0166-445X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8994
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.City Univ Hong Kong, Ctr Coastal Pollut & Conservat, Kowloon, Hong Kong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhou, BS; Liu, WH; Siu, WHL; O'Toole, D; Lam, PKS; Wu, RSS.Exposure of spermatozoa to duroquinone may impair reproduction of the common carp (Cyprinus carpio) through oxidative stress,AQUATIC TOXICOLOGY,2006,77(2):136-142
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