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Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line
Huang, XH; Huang, YH; Yuan, XP; Zhang, QY; Zhang, QY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2006-05-01
Source PublicationJOURNAL OF VIROLOGICAL METHODS
ISSN0166-0934
Volume133Issue:2Pages:117-123
AbstractRana grylio virus (RGV), a Ranavirus belonging to the family Iridoviridae, assembles in the viromatrix which is a factory for viral genome replication and particle assembly. Ultrastructural studies of the viromatrix will clarify the pathway of assembly. The viromatrix and quantitative changes in RGV infected epithelipma papulosum cyprini (EPC) cells, one of fish cell lines, were studied by electron microscopy. It was shown that viromatrices were adjacent to the nucleus, and the electron density was lower than that of the surrounding cytoplasm. The viromatrix contained virus particles with different forms, electron-dense materials and amorphous structures which included tubules and membranous materials. Tubules were often observed in direct continuity with empty capsids. Several bundles of intermediate filaments were seen alongside the viromatrix and crystalline aggregates. Large clusters of mitochondria occurred in proximity to viromatrix. A total of 990 cells profiles were examined. The results showed that 394 cells contained viromatrix: 89.3% contained one, and 10.7% contained two to four viromatrices. The number of viromatrices increased gradually and reached a peak at 16 h p.i. The viromatrix area at 24 h p.i. increased up to 7.4 +/- 0.69 mu m(2) which was three-times lower than that at 6 h p.i. The number of empty capsids within viromatrix was generally more than that of "full" particles at different time points, and there was a strong positive correlation between them. (c) 2005 Elsevier B.V. All rights reserved.; Rana grylio virus (RGV), a Ranavirus belonging to the family Iridoviridae, assembles in the viromatrix which is a factory for viral genome replication and particle assembly. Ultrastructural studies of the viromatrix will clarify the pathway of assembly. The viromatrix and quantitative changes in RGV infected epithelipma papulosum cyprini (EPC) cells, one of fish cell lines, were studied by electron microscopy. It was shown that viromatrices were adjacent to the nucleus, and the electron density was lower than that of the surrounding cytoplasm. The viromatrix contained virus particles with different forms, electron-dense materials and amorphous structures which included tubules and membranous materials. Tubules were often observed in direct continuity with empty capsids. Several bundles of intermediate filaments were seen alongside the viromatrix and crystalline aggregates. Large clusters of mitochondria occurred in proximity to viromatrix. A total of 990 cells profiles were examined. The results showed that 394 cells contained viromatrix: 89.3% contained one, and 10.7% contained two to four viromatrices. The number of viromatrices increased gradually and reached a peak at 16 h p.i. The viromatrix area at 24 h p.i. increased up to 7.4 +/- 0.69 mu m(2) which was three-times lower than that at 6 h p.i. The number of empty capsids within viromatrix was generally more than that of "full" particles at different time points, and there was a strong positive correlation between them. (c) 2005 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordIridovirus Rgv Viromatrix Ultrastructure Quantitative Analysis
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China
Subject AreaBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
DOI10.1016/j.jviromet.2005.10.029
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Virology
WOS SubjectBiochemical Research Methods ; Biotechnology & Applied Microbiology ; Virology
WOS IDWOS:000236956200001
WOS KeywordSWINE-FEVER VIRUS ; QUANTITATIVE ULTRASTRUCTURAL ANALYSIS ; VACCINIA VIRUS ; ENDOPLASMIC-RETICULUM ; INTERMEDIATE COMPARTMENT ; ASSEMBLY SITES ; INFECTED CELLS ; MICROTUBULES ; MORPHOGENESIS ; IRIDOVIRUS
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Cited Times:40[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8990
Collection期刊论文
Corresponding AuthorZhang, QY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 10039, Peoples R China
Recommended Citation
GB/T 7714
Huang, XH,Huang, YH,Yuan, XP,et al. Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line[J]. JOURNAL OF VIROLOGICAL METHODS,2006,133(2):117-123.
APA Huang, XH,Huang, YH,Yuan, XP,Zhang, QY,&Zhang, QY, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2006).Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line.JOURNAL OF VIROLOGICAL METHODS,133(2),117-123.
MLA Huang, XH,et al."Electron microscopic examination of the viromatrix of Rana grylio virus in a fish cell line".JOURNAL OF VIROLOGICAL METHODS 133.2(2006):117-123.
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