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Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock
Dong, CW; Zhang, YB; Zhang, QY; Gui, HF; Gui, HF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2006-08-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume21Issue:2Pages:146-158
AbstractHeat shock proteins (Hsps) are a family of highly conserved cellular proteins present in all organisms, mediating a range of essential housekeeping and cytoprotective functions as well-known molecular chaperons and recently as regulators of the immune response. By subtractive suppression hybridization, three Hsp40 homologues have been identified in the flounder (Paralichthys olivaceus) embryonic cells (FEC) after treatment with UV-inactivated turbot (Scophthalmus maximus L.) rhabdovirus (SMRV), termed PoHsp40A4, PoHsp40B6 and PoHsp40B11, whose encoded proteins all possess the conserved DnaJ domain, a signature motif of the Hsp40 family. Based on different protein structure and phylogenetic analysis, they can be categorized into two subfamilies, PoHsp40A4 for Type I Hsp40, PoHsp40B6 and PoHsp40B11 for Type 11 Hsp40. Further expression analysis revealed two very different types of kinetics in response either to heat shock or to virus infection, with a marked induction for PoHsp4OA4 and a weak one for both PoHsp40B6 and PoHsp40B11. A very distinct tissue distribution of mRNA was also revealed among the three genes, even between PoHsp40B6 and PoHsp40B11. This is the first report on the transcriptional induction of Hsp40 in virally stimulated fish cells, and the differential expressions might reflect their different roles in unstressed and stressed cells. (c) 2005 Elsevier Ltd. All rights reserved.; Heat shock proteins (Hsps) are a family of highly conserved cellular proteins present in all organisms, mediating a range of essential housekeeping and cytoprotective functions as well-known molecular chaperons and recently as regulators of the immune response. By subtractive suppression hybridization, three Hsp40 homologues have been identified in the flounder (Paralichthys olivaceus) embryonic cells (FEC) after treatment with UV-inactivated turbot (Scophthalmus maximus L.) rhabdovirus (SMRV), termed PoHsp40A4, PoHsp40B6 and PoHsp40B11, whose encoded proteins all possess the conserved DnaJ domain, a signature motif of the Hsp40 family. Based on different protein structure and phylogenetic analysis, they can be categorized into two subfamilies, PoHsp40A4 for Type I Hsp40, PoHsp40B6 and PoHsp40B11 for Type 11 Hsp40. Further expression analysis revealed two very different types of kinetics in response either to heat shock or to virus infection, with a marked induction for PoHsp4OA4 and a weak one for both PoHsp40B6 and PoHsp40B11. A very distinct tissue distribution of mRNA was also revealed among the three genes, even between PoHsp40B6 and PoHsp40B11. This is the first report on the transcriptional induction of Hsp40 in virally stimulated fish cells, and the differential expressions might reflect their different roles in unstressed and stressed cells. (c) 2005 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordHeat Shock Protein (Hsp) 40 Paralichthys Olivaceus Scophthalmus Maximus Rhabdovirus Differential Expression Heat Shock
DepartmentChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI10.1016/j.fsi.2005.11.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000237633600004
WOS KeywordINDUCED SIGNAL-TRANSDUCTION ; ESCHERICHIA-COLI DNAJ ; FINGER-LIKE DOMAIN ; MOLECULAR CHAPERONE ; RAINBOW-TROUT ; CELLULAR INHIBITOR ; DENDRITIC CELLS ; ATPASE ACTIVITY ; MESSENGER-RNA ; PROTEIN EXPRESSION
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Cited Times:36[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8972
Collection期刊论文
Corresponding AuthorGui, HF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Dong, CW,Zhang, YB,Zhang, QY,et al. Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock[J]. FISH & SHELLFISH IMMUNOLOGY,2006,21(2):146-158.
APA Dong, CW,Zhang, YB,Zhang, QY,Gui, HF,&Gui, HF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock.FISH & SHELLFISH IMMUNOLOGY,21(2),146-158.
MLA Dong, CW,et al."Differential expression of three Paralichthys olivaceus Hsp40 genes in responses to virus infection and heat shock".FISH & SHELLFISH IMMUNOLOGY 21.2(2006):146-158.
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