| Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae | |
| Wang, Chunfang; Xie, Shouqi; Zhu, Xiaoming; Lei, Wu; Yang, Yunxia; Liu, Hankang; Xie, SQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China | |
| 2006-04-28 | |
| Source Publication | AQUACULTURE
 |
| ISSN | 0044-8486 |
| Volume | 254Issue:1-4Pages:554-562 |
| Abstract | The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Petteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly hatched Artemia, unenriched) was included as a control. The effects of age on enzyme activity and mRNA were as follows: pepsin and trypsin activity in all treatment groups showed a significant (P < 0.05) increase at the beginning and decrease later although the timing of decrease was not the same among treatment groups and between the digestive enzymes. Pepsin and trypsin mRNA level followed the pattern of their respective enzyme changes. Age significantly affected amylase activity (P < 0.05) while age had no effect on amylase mRNA during the experimental period. The four diets significantly (P < 0.05) affected activity and mRNA level of pepsin and trypsin. Diets did not affect amylase activity or mRNA level. These results suggest that the effects of age on pepsin and trypsin gene expressions are at the transcriptional level. Dietary protein level does affect pepsin and trypsin gene expression in the early life of P. fulvidraco. There were no transcriptional effects on amylase gene expression. (c) 2005 Elsevier B.V. All rights reserved.; The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Petteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly hatched Artemia, unenriched) was included as a control. The effects of age on enzyme activity and mRNA were as follows: pepsin and trypsin activity in all treatment groups showed a significant (P < 0.05) increase at the beginning and decrease later although the timing of decrease was not the same among treatment groups and between the digestive enzymes. Pepsin and trypsin mRNA level followed the pattern of their respective enzyme changes. Age significantly affected amylase activity (P < 0.05) while age had no effect on amylase mRNA during the experimental period. The four diets significantly (P < 0.05) affected activity and mRNA level of pepsin and trypsin. Diets did not affect amylase activity or mRNA level. These results suggest that the effects of age on pepsin and trypsin gene expressions are at the transcriptional level. Dietary protein level does affect pepsin and trypsin gene expression in the early life of P. fulvidraco. There were no transcriptional effects on amylase gene expression. (c) 2005 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Pelteobagrus Fulvidraco Pepsin Trypsin Amylase Activity Mrna |
| Department | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Shanghai Univ, Aquaculture Div, E Inst, Shanghai, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| Subject Area | Fisheries ; Marine & Freshwater Biology |
| DOI | 10.1016/j.aquaculture.2005.11.036 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Fisheries ; Marine & Freshwater Biology |
| WOS Subject | Fisheries ; Marine & Freshwater Biology |
| WOS ID | WOS:000237838800058 |
| WOS Keyword | PENAEUS-VANNAMEI CRUSTACEA ; MESSENGER-RNA EXPRESSION ; DIFFERENT FOOD RATIONS ; WINTER FLOUNDER ; PLEURONECTES-AMERICANUS ; SOLEA-SENEGALENSIS ; CRASSOSTREA-GIGAS ; CLUPEA-HARENGUS ; COMPOUND DIETS ; ONTOGENY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8962 |
| Collection | 期刊论文 |
| Corresponding Author | Xie, SQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Shanghai Univ, Aquaculture Div, E Inst, Shanghai, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Chunfang,Xie, Shouqi,Zhu, Xiaoming,et al. Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae[J]. AQUACULTURE,2006,254(1-4):554-562. |
| APA | Wang, Chunfang.,Xie, Shouqi.,Zhu, Xiaoming.,Lei, Wu.,Yang, Yunxia.,...&Xie, SQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.(2006).Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae.AQUACULTURE,254(1-4),554-562. |
| MLA | Wang, Chunfang,et al."Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae".AQUACULTURE 254.1-4(2006):554-562. |
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