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学科主题: Fisheries; Marine & Freshwater Biology
题名: Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae
作者: Wang, Chunfang; Xie, Shouqi; Zhu, Xiaoming; Lei, Wu; Yang, Yunxia; Liu, Hankang
通讯作者: Xie, SQ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
关键词: Pelteobagrus fulvidraco ; pepsin ; trypsin ; amylase ; activity ; mRNA
刊名: AQUACULTURE
发表日期: 2006-04-28
DOI: 10.1016/j.aquaculture.2005.11.036
卷: 254, 期:1-4, 页:554-562
收录类别: SCI
文章类型: Article
部门归属: Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; Shanghai Univ, Aquaculture Div, E Inst, Shanghai, Peoples R China; Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Fisheries ; Marine & Freshwater Biology
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology
摘要: The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Petteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly hatched Artemia, unenriched) was included as a control. The effects of age on enzyme activity and mRNA were as follows: pepsin and trypsin activity in all treatment groups showed a significant (P < 0.05) increase at the beginning and decrease later although the timing of decrease was not the same among treatment groups and between the digestive enzymes. Pepsin and trypsin mRNA level followed the pattern of their respective enzyme changes. Age significantly affected amylase activity (P < 0.05) while age had no effect on amylase mRNA during the experimental period. The four diets significantly (P < 0.05) affected activity and mRNA level of pepsin and trypsin. Diets did not affect amylase activity or mRNA level. These results suggest that the effects of age on pepsin and trypsin gene expressions are at the transcriptional level. Dietary protein level does affect pepsin and trypsin gene expression in the early life of P. fulvidraco. There were no transcriptional effects on amylase gene expression. (c) 2005 Elsevier B.V. All rights reserved.
英文摘要: The present research studied the effects of age and dietary protein level on pepsin, trypsin and amylase activity and their mRNA level in Petteobagrus fulvidraco larvae from 3 to 26 days after hatch (DAH). Three DAH larvae were fed three isoenergetic diets, containing 42.8% (CP 43), 47.3% (CP 47) and 52.8% (CP 53) crude protein. Live food (newly hatched Artemia, unenriched) was included as a control. The effects of age on enzyme activity and mRNA were as follows: pepsin and trypsin activity in all treatment groups showed a significant (P < 0.05) increase at the beginning and decrease later although the timing of decrease was not the same among treatment groups and between the digestive enzymes. Pepsin and trypsin mRNA level followed the pattern of their respective enzyme changes. Age significantly affected amylase activity (P < 0.05) while age had no effect on amylase mRNA during the experimental period. The four diets significantly (P < 0.05) affected activity and mRNA level of pepsin and trypsin. Diets did not affect amylase activity or mRNA level. These results suggest that the effects of age on pepsin and trypsin gene expressions are at the transcriptional level. Dietary protein level does affect pepsin and trypsin gene expression in the early life of P. fulvidraco. There were no transcriptional effects on amylase gene expression. (c) 2005 Elsevier B.V. All rights reserved.
关键词[WOS]: PENAEUS-VANNAMEI CRUSTACEA ; MESSENGER-RNA EXPRESSION ; DIFFERENT FOOD RATIONS ; WINTER FLOUNDER ; PLEURONECTES-AMERICANUS ; SOLEA-SENEGALENSIS ; CRASSOSTREA-GIGAS ; CLUPEA-HARENGUS ; COMPOUND DIETS ; ONTOGENY
语种: 英语
WOS记录号: WOS:000237838800058
ISSN号: 0044-8486
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8962
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Shanghai Univ, Aquaculture Div, E Inst, Shanghai, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China

Recommended Citation:
Wang, CF; Xie, SQ; Zhu, XM; Wu, L; Yang, YX; Liu, HK.Effects of age and dietary protein level on digestive enzyme activity and gene expression of Pelteobagrus fulvidraco larvae,AQUACULTURE,2006,254(1-4):554-562
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