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Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization
Miao, W; Yu, T; Orias, E; Wan, ML; Fu, CJ; Miao, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2006-06-01
Source PublicationENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
Volume8Issue:6Pages:1122-1129
AbstractThe insecticide dichlorodiphenyltrichloroethane (DDT) is persistent in the environment, and continues to cause health problems. Tetrahymena has potential as a model organism for assaying low levels of DDT and for analysing the mechanisms of its toxicity. We constructed the suppression subtractive hybridization library of T thermophila exposed to DDT, and screened out 90 Expressed Sequence Tags whose expressions were significantly up- or downregulated with DDT treatment. From this, a series of important genes related to the DDT metabolism and detoxification were discovered, such as P450 gene, glutathione S-transferase gene and sterol carrier protein 2 gene. Furthermore, their expressions under different concentrations of DDT treatment were detected by real-time fluorescent quantitative PCR. The results show that Tetrahymena is a relevant and useful model organism for detecting DDT in the environment and for discovering biomarkers that can be used to develop specific bio-reporters at the molecular and genomic levels.; The insecticide dichlorodiphenyltrichloroethane (DDT) is persistent in the environment, and continues to cause health problems. Tetrahymena has potential as a model organism for assaying low levels of DDT and for analysing the mechanisms of its toxicity. We constructed the suppression subtractive hybridization library of T thermophila exposed to DDT, and screened out 90 Expressed Sequence Tags whose expressions were significantly up- or downregulated with DDT treatment. From this, a series of important genes related to the DDT metabolism and detoxification were discovered, such as P450 gene, glutathione S-transferase gene and sterol carrier protein 2 gene. Furthermore, their expressions under different concentrations of DDT treatment were detected by real-time fluorescent quantitative PCR. The results show that Tetrahymena is a relevant and useful model organism for detecting DDT in the environment and for discovering biomarkers that can be used to develop specific bio-reporters at the molecular and genomic levels.
SubtypeArticle
KeywordSterol Carrier Protein-2 Tetrahymena-thermophila Caenorhabditis-elegans Cholesterol Liver Pcr
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Chinese Acad Fishery Sci, Key Lab Fisheries Environm & Aquaculture Ecol, Shanghai 200090, Peoples R China; Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA; Chinese Acad Sci, Grad Sch, Wuhan Branch, Wuhan 430072, Peoples R China
Subject AreaMicrobiology
DOI10.1111/j.1462-2920.2006.00988.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000237949500018
WOS KeywordSTEROL CARRIER PROTEIN-2 ; TETRAHYMENA-THERMOPHILA ; CAENORHABDITIS-ELEGANS ; CHOLESTEROL ; LIVER ; PCR
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8958
Collection期刊论文
Corresponding AuthorMiao, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Fishery Sci, Key Lab Fisheries Environm & Aquaculture Ecol, Shanghai 200090, Peoples R China
3.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
4.Chinese Acad Sci, Grad Sch, Wuhan Branch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Miao, W,Yu, T,Orias, E,et al. Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization[J]. ENVIRONMENTAL MICROBIOLOGY,2006,8(6):1122-1129.
APA Miao, W,Yu, T,Orias, E,Wan, ML,Fu, CJ,&Miao, W, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2006).Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization.ENVIRONMENTAL MICROBIOLOGY,8(6),1122-1129.
MLA Miao, W,et al."Identification of differentially expressed genes in Tetrahmena thermophila in response to dichlorodiphenyltrichloroethane (DDT) by suppression subtractive hybridization".ENVIRONMENTAL MICROBIOLOGY 8.6(2006):1122-1129.
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