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EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)
Zhou, Li; Yao, Bo; Xia, Wei; Li, Chuang-Ju; Wang, Yang; Shi, Yao-Hua; Gui, Jian-Fang; Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
2006-06-15
Source PublicationAQUACULTURE
ISSN0044-8486
Volume256Issue:1-4Pages:129-139
AbstractThe orange-spotted grouper, Epinephelus coioides, is an important marine aquaculture fish, but its large-scale aquaculture has been hindered by the rarity of natural males because it is a protogynous hermaphroditic fish. Hypothalamus-pituitary-gonad is an important endocrine axis in regulating reproduction and sex differentiation. To reveal the molecular mechanism of hypothalamic physiological functions, we performed he studies on identification of genes expressed in the hypothalamus of male orange-spotted grouper using EST and RT-PCR strategy. A total of 1006 ESTs were sequenced, and 402 (39.96%) clones were identified as known genes and 604 (60.04%) as unknown genes. The 402 clones of known gene products represent transcripts of 18 1 genes. Moreover, the expression patterns of 26 unknown genes were analyzed in various tissues, such as liver, kidney, spleen, fat, heart, muscle, pituitary, hypothalamus, telencephalon, cerebellum, midbrain, medulla oblongata, ovary and testes. Five different categories of expression patterns were observed from them. Several unknown ESTs, such as DN551996, DN551998, DN552082, and DN552070, were detected to be hypothalamus-specific, brains-specific, or hypothalamus and gonad-specific genes. Interestingly, DN551996, not only exhibiting expression differences between ovary and testis, but also showing sex-dependent differences in hypothalamus of grouper, might play significant role in grouper reproduction or sex inversion. Further functional studies on these genes will provide more information on molecule regulation mechanism of sex inversion in groupers. (c) 2006 Elsevier B.V All fights reserved.; The orange-spotted grouper, Epinephelus coioides, is an important marine aquaculture fish, but its large-scale aquaculture has been hindered by the rarity of natural males because it is a protogynous hermaphroditic fish. Hypothalamus-pituitary-gonad is an important endocrine axis in regulating reproduction and sex differentiation. To reveal the molecular mechanism of hypothalamic physiological functions, we performed he studies on identification of genes expressed in the hypothalamus of male orange-spotted grouper using EST and RT-PCR strategy. A total of 1006 ESTs were sequenced, and 402 (39.96%) clones were identified as known genes and 604 (60.04%) as unknown genes. The 402 clones of known gene products represent transcripts of 18 1 genes. Moreover, the expression patterns of 26 unknown genes were analyzed in various tissues, such as liver, kidney, spleen, fat, heart, muscle, pituitary, hypothalamus, telencephalon, cerebellum, midbrain, medulla oblongata, ovary and testes. Five different categories of expression patterns were observed from them. Several unknown ESTs, such as DN551996, DN551998, DN552082, and DN552070, were detected to be hypothalamus-specific, brains-specific, or hypothalamus and gonad-specific genes. Interestingly, DN551996, not only exhibiting expression differences between ovary and testis, but also showing sex-dependent differences in hypothalamus of grouper, might play significant role in grouper reproduction or sex inversion. Further functional studies on these genes will provide more information on molecule regulation mechanism of sex inversion in groupers. (c) 2006 Elsevier B.V All fights reserved.
SubtypeArticle
KeywordExpressed Sequence Tags Hypothalamus Grouper Epinephelus Coioides Sex Inversion
DepartmentChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
Subject AreaFisheries ; Marine & Freshwater Biology
DOI10.1016/j.aquaculture.2006.01.022
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology
WOS SubjectFisheries ; Marine & Freshwater Biology
WOS IDWOS:000238394400015
WOS KeywordCATFISH ICTALURUS-PUNCTATUS ; SALMON SALMO-SALAR ; CHANNEL CATFISH ; SEQUENCE TAGS ; SEX-CHANGE ; PROTOGYNOUS GROUPER ; TRANSCRIPTOME ANALYSIS ; BRAIN ; LIVER ; SPLEEN
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Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/152342/8950
Collection期刊论文
Corresponding AuthorGui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Li,Yao, Bo,Xia, Wei,et al. EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)[J]. AQUACULTURE,2006,256(1-4):129-139.
APA Zhou, Li.,Yao, Bo.,Xia, Wei.,Li, Chuang-Ju.,Wang, Yang.,...&Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol, Inst Hydrobiol,Grad Sch, Wuhan 430072, Peoples R China.(2006).EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides).AQUACULTURE,256(1-4),129-139.
MLA Zhou, Li,et al."EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)".AQUACULTURE 256.1-4(2006):129-139.
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