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学科主题: Biochemistry & Molecular Biology
题名: Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera
作者: Wan, YY; Du, YM; Yang, FX; Xu, Y; Chen, RZ; Kennedy, JF
通讯作者: Du, YM, Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China
关键词: Rhus vernicifera ; Rhus laccase ; isoenzymes ; purification ; continuous gradient elation chromatography ; MALDI-TOF-MS
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2006-05-30
DOI: 10.1016/j.ijbiomac.2006.02.019
卷: 38, 期:3-5, 页:232-240
收录类别: SCI
文章类型: Article
部门归属: Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China; Univ Birmingham, Sch Chem Sci, Birmingham Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England; Univ Birmingham, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Continuous gradient elution chromatography (CGEC) was employed to purify and separate enzymes and polysaccharides from the sap of Rhus vernicifera Chinese lacquer tree. There are three different molecules with laccase enzyme activity. Two are enzymes of each other (L1, and L2), whereas the third (RL) is an entirely separate entity. Two polysaccharides (GP1 and GP2) were also found. The Rhus laccase (RL), and isoenzymes L1 and L2, have peak molecular masses of 109,100, 120,000, 103,000 respectively; each has four copper atoms per molecule, and the pI values were 8.2, 8.6, and 9.1, respectively. The structure of the laccases was studied by Fourier-transform infrared (FT-IR) and Matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF) mass spectrometry. The typical amide I (1646 cm(-1)) and amide II (1545 cm(-1)) bands were observed. The results from MALDI-TOF were similar to those from CGEC, but the molecular mass from the MALDI-TOF was significantly different from that obtained from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (c) 2006 Elsevier B.V. All rights reserved.
英文摘要: Continuous gradient elution chromatography (CGEC) was employed to purify and separate enzymes and polysaccharides from the sap of Rhus vernicifera Chinese lacquer tree. There are three different molecules with laccase enzyme activity. Two are enzymes of each other (L1, and L2), whereas the third (RL) is an entirely separate entity. Two polysaccharides (GP1 and GP2) were also found. The Rhus laccase (RL), and isoenzymes L1 and L2, have peak molecular masses of 109,100, 120,000, 103,000 respectively; each has four copper atoms per molecule, and the pI values were 8.2, 8.6, and 9.1, respectively. The structure of the laccases was studied by Fourier-transform infrared (FT-IR) and Matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF) mass spectrometry. The typical amide I (1646 cm(-1)) and amide II (1545 cm(-1)) bands were observed. The results from MALDI-TOF were similar to those from CGEC, but the molecular mass from the MALDI-TOF was significantly different from that obtained from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (c) 2006 Elsevier B.V. All rights reserved.
关键词[WOS]: BLUE COPPER PROTEINS ; LAC TREE ; SECONDARY STRUCTURE ; CHEMICAL-MODIFICATION ; MULTICOPPER OXIDASES ; PYCNOPORUS-COCCINEUS ; CONIFERYL ALCOHOL ; ASCORBATE OXIDASE ; LACCASE ; SPECTROSCOPY
语种: 英语
WOS记录号: WOS:000238334000011
ISSN号: 0141-8130
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/152342/8940
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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作者单位: 1.Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China
4.Univ Birmingham, Sch Chem Sci, Birmingham Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England
5.Univ Birmingham, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England

Recommended Citation:
Wan, YY; Du, YM; Yang, FX; Xu, Y; Chen, RZ; Kennedy, JF.Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera,INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2006,38(3-5):232-240
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