| Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera | |
| Wan, YY; Du, YM; Yang, FX; Xu, Y; Chen, RZ; Kennedy, JF; Du, YM, Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China | |
| 2006-05-30 | |
| Source Publication | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| ISSN | 0141-8130 |
| Volume | 38Issue:3-5Pages:232-240 |
| Abstract | Continuous gradient elution chromatography (CGEC) was employed to purify and separate enzymes and polysaccharides from the sap of Rhus vernicifera Chinese lacquer tree. There are three different molecules with laccase enzyme activity. Two are enzymes of each other (L1, and L2), whereas the third (RL) is an entirely separate entity. Two polysaccharides (GP1 and GP2) were also found. The Rhus laccase (RL), and isoenzymes L1 and L2, have peak molecular masses of 109,100, 120,000, 103,000 respectively; each has four copper atoms per molecule, and the pI values were 8.2, 8.6, and 9.1, respectively. The structure of the laccases was studied by Fourier-transform infrared (FT-IR) and Matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF) mass spectrometry. The typical amide I (1646 cm(-1)) and amide II (1545 cm(-1)) bands were observed. The results from MALDI-TOF were similar to those from CGEC, but the molecular mass from the MALDI-TOF was significantly different from that obtained from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (c) 2006 Elsevier B.V. All rights reserved.; Continuous gradient elution chromatography (CGEC) was employed to purify and separate enzymes and polysaccharides from the sap of Rhus vernicifera Chinese lacquer tree. There are three different molecules with laccase enzyme activity. Two are enzymes of each other (L1, and L2), whereas the third (RL) is an entirely separate entity. Two polysaccharides (GP1 and GP2) were also found. The Rhus laccase (RL), and isoenzymes L1 and L2, have peak molecular masses of 109,100, 120,000, 103,000 respectively; each has four copper atoms per molecule, and the pI values were 8.2, 8.6, and 9.1, respectively. The structure of the laccases was studied by Fourier-transform infrared (FT-IR) and Matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF) mass spectrometry. The typical amide I (1646 cm(-1)) and amide II (1545 cm(-1)) bands were observed. The results from MALDI-TOF were similar to those from CGEC, but the molecular mass from the MALDI-TOF was significantly different from that obtained from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (c) 2006 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Rhus Vernicifera Rhus Laccase Isoenzymes Purification Continuous Gradient Elation Chromatography Maldi-tof-ms |
| Department | Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China; Univ Birmingham, Sch Chem Sci, Birmingham Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England; Univ Birmingham, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England |
| Subject Area | Biochemistry & Molecular Biology |
| DOI | 10.1016/j.ijbiomac.2006.02.019 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology |
| WOS Subject | Biochemistry & Molecular Biology |
| WOS ID | WOS:000238334000011 |
| WOS Keyword | BLUE COPPER PROTEINS ; LAC TREE ; SECONDARY STRUCTURE ; CHEMICAL-MODIFICATION ; MULTICOPPER OXIDASES ; PYCNOPORUS-COCCINEUS ; CONIFERYL ALCOHOL ; ASCORBATE OXIDASE ; LACCASE ; SPECTROSCOPY |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8940 |
| Collection | 期刊论文 |
| Corresponding Author | Du, YM, Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China |
| Affiliation | 1.Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China 4.Univ Birmingham, Sch Chem Sci, Birmingham Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England 5.Univ Birmingham, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England |
| Recommended Citation GB/T 7714 | Wan, YY,Du, YM,Yang, FX,et al. Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2006,38(3-5):232-240. |
| APA | Wan, YY.,Du, YM.,Yang, FX.,Xu, Y.,Chen, RZ.,...&Du, YM, Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China.(2006).Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,38(3-5),232-240. |
| MLA | Wan, YY,et al."Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 38.3-5(2006):232-240. |
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