Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China; Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China; Univ Birmingham, Sch Chem Sci, Birmingham Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England; Univ Birmingham, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England
WOS标题词:
Science & Technology
; Life Sciences & Biomedicine
类目[WOS]:
Biochemistry & Molecular Biology
研究领域[WOS]:
Biochemistry & Molecular Biology
摘要:
Continuous gradient elution chromatography (CGEC) was employed to purify and separate enzymes and polysaccharides from the sap of Rhus vernicifera Chinese lacquer tree. There are three different molecules with laccase enzyme activity. Two are enzymes of each other (L1, and L2), whereas the third (RL) is an entirely separate entity. Two polysaccharides (GP1 and GP2) were also found. The Rhus laccase (RL), and isoenzymes L1 and L2, have peak molecular masses of 109,100, 120,000, 103,000 respectively; each has four copper atoms per molecule, and the pI values were 8.2, 8.6, and 9.1, respectively. The structure of the laccases was studied by Fourier-transform infrared (FT-IR) and Matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF) mass spectrometry. The typical amide I (1646 cm(-1)) and amide II (1545 cm(-1)) bands were observed. The results from MALDI-TOF were similar to those from CGEC, but the molecular mass from the MALDI-TOF was significantly different from that obtained from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (c) 2006 Elsevier B.V. All rights reserved.
英文摘要:
Continuous gradient elution chromatography (CGEC) was employed to purify and separate enzymes and polysaccharides from the sap of Rhus vernicifera Chinese lacquer tree. There are three different molecules with laccase enzyme activity. Two are enzymes of each other (L1, and L2), whereas the third (RL) is an entirely separate entity. Two polysaccharides (GP1 and GP2) were also found. The Rhus laccase (RL), and isoenzymes L1 and L2, have peak molecular masses of 109,100, 120,000, 103,000 respectively; each has four copper atoms per molecule, and the pI values were 8.2, 8.6, and 9.1, respectively. The structure of the laccases was studied by Fourier-transform infrared (FT-IR) and Matrix-assisted laser desorption/ionization time-of flight (MALDI-TOF) mass spectrometry. The typical amide I (1646 cm(-1)) and amide II (1545 cm(-1)) bands were observed. The results from MALDI-TOF were similar to those from CGEC, but the molecular mass from the MALDI-TOF was significantly different from that obtained from sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (c) 2006 Elsevier B.V. All rights reserved.
1.Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China 4.Univ Birmingham, Sch Chem Sci, Birmingham Carbohydrate & Prot Technol Grp, Birmingham B15 2TT, W Midlands, England 5.Univ Birmingham, Chembiotech Labs, Birmingham B15 2SQ, W Midlands, England
Recommended Citation:
Wan, YY; Du, YM; Yang, FX; Xu, Y; Chen, RZ; Kennedy, JF.Purification and characterization of hydrosoluble components from the sap of Chinese lacquer tree Rhus vernicifera,INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2006,38(3-5):232-240
