| Effects of salt stress on carbohydrate metabolism in desert soil alga Microcoleus vaginatus Gom. | |
| Chen, Lan-Zhou; Li, Dun-Hai; Song, Li-Rong; Hu, Chun-Xiang; Wang, Gao-Hong; Liu, Yong-Ding; Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China | |
| 2006-08-01 | |
| Source Publication | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| ISSN | 1672-9072 |
| Volume | 48Issue:8Pages:914-919 |
| Abstract | The effects of salt stress on carbohydrate metabolism in Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crusts, were investigated in the present study. Extracellular total carbohydrates and exopolysaccharides (EPS) in the culture medium produced by M. vaginatus increased significantly during the growth phase and reached a maximum during the stationary phase. The production of extracellular carbohydrates also significantly increased under higher salt concentrations, which was attributed to an increase in low molecular weight carbohydrates. In the presence of NaCl, the production of cellular total carbohydrates decreased and photosynthetic activity was impaired, whereas cellular reducing sugars, water-soluble sugars and sucrose content and sucrose phosphate synthase activity increased, reaching a maximum in the presence of 200 mmol/L NaCl. These parameters were restored to original levels when the algae were transferred to a non-saline medium. Sodium and K+ concentrations of stressed cells decreased significantly and H+-ATPase activity increased after the addition of exogenous sucrose or EPS. The results suggest that EPS and sucrose are synthesized to maintain the cellular osmotic equilibrium between the intra- and extracellular environment, thus protecting algal cells from osmotic damage, which was attributed to the selective exclusion of cellular Na+ and K+ by H+-ATPase.; The effects of salt stress on carbohydrate metabolism in Microcoleus vaginatus Gom., a cyanobacterium isolated from desert algal crusts, were investigated in the present study. Extracellular total carbohydrates and exopolysaccharides (EPS) in the culture medium produced by M. vaginatus increased significantly during the growth phase and reached a maximum during the stationary phase. The production of extracellular carbohydrates also significantly increased under higher salt concentrations, which was attributed to an increase in low molecular weight carbohydrates. In the presence of NaCl, the production of cellular total carbohydrates decreased and photosynthetic activity was impaired, whereas cellular reducing sugars, water-soluble sugars and sucrose content and sucrose phosphate synthase activity increased, reaching a maximum in the presence of 200 mmol/L NaCl. These parameters were restored to original levels when the algae were transferred to a non-saline medium. Sodium and K+ concentrations of stressed cells decreased significantly and H+-ATPase activity increased after the addition of exogenous sucrose or EPS. The results suggest that EPS and sucrose are synthesized to maintain the cellular osmotic equilibrium between the intra- and extracellular environment, thus protecting algal cells from osmotic damage, which was attributed to the selective exclusion of cellular Na+ and K+ by H+-ATPase. |
| Subtype | Article |
| Keyword | Carbohydrate Metabolism Desert Cyanobacteria Exopolysaccharides (Eps) Osmotic Equilibrium Salt Stress |
| Department | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430072, Peoples R China |
| Subject Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS Subject | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS ID | WOS:000239510200005 |
| WOS Keyword | STABILIZATION ; DESICCATION ; SOLUTES ; SUCROSE ; CRUSTS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/152342/8892 |
| Collection | 期刊论文 |
| Corresponding Author | Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| Affiliation | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430072, Peoples R China |
| Recommended Citation GB/T 7714 | Chen, Lan-Zhou,Li, Dun-Hai,Song, Li-Rong,et al. Effects of salt stress on carbohydrate metabolism in desert soil alga Microcoleus vaginatus Gom.[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2006,48(8):914-919. |
| APA | Chen, Lan-Zhou.,Li, Dun-Hai.,Song, Li-Rong.,Hu, Chun-Xiang.,Wang, Gao-Hong.,...&Liu, YD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.(2006).Effects of salt stress on carbohydrate metabolism in desert soil alga Microcoleus vaginatus Gom..JOURNAL OF INTEGRATIVE PLANT BIOLOGY,48(8),914-919. |
| MLA | Chen, Lan-Zhou,et al."Effects of salt stress on carbohydrate metabolism in desert soil alga Microcoleus vaginatus Gom.".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 48.8(2006):914-919. |
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